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Human L-Selectin ELISA kit

  • 中文名称：
    人L选择素(L-Selectin/CD62L)酶联免疫试剂盒
  • 货号：
    CSB-E04653h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        SELL（L-selectin）：SELL是一种细胞黏附分子，属于选择素家族，广泛表达于白细胞表面，参与白细胞与内皮细胞的黏附和转移。SELL通过与其配体GlyCAM-1和CD34结合，参与免疫细胞在淋巴结和炎症部位的定向迁移。SELL的表达受到多种因素的调控，如炎症因子、细胞因子、荷尔蒙等。SELL的异常表达与多种疾病状态相关，如癌症、自身免疫性疾病等。
        j9九游会登录入口首页生物所提供的Human L-Selectin ELISA kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的SELL，其灵敏度为0.195 ng/mL，检测范围为0.78 ng/mL-50 ng/mL。
     
  • 别名：
    SELL; LNHR; LYAM1; L-selectin; CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; TQ1; gp90-MEL; CD antigen CD62L
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Stem Cells
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human L-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:5 Average % 90  
    Range % 84-95  
    1:10 Average % 92  
    Range % 87-96  
    1:20 Average % 95  
    Range % 90-102  
    1:40 Average % 94  
    Range % 86-101  
  • 回收率：
    The recovery of human L-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 86-94  
    EDTA plasma (n=4) 92 85-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    50 2.498 2.662 2.580 2.409  
    25 2.166 2.252 2.209 2.038  
    12.5 1.651 1.669 1.660 1.489  
    6.25 0.932 0.967 0.950 0.779  
    3.12 0.550 0.514 0.532 0.361  
    1.56 0.363 0.352 0.358 0.187  
    0.78 0.265 0.284 0.275 0.104  
    0 0.173 0.169 0.171 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    SELL（淋巴细胞选择素）在淋巴细胞与血管内皮细胞之间发挥作用
  • 功能：
    Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
  • 基因功能参考文献：
    1. L-selectin (CD62L) has been identified as an HIV-1 adhesion receptor on CD4+ T cells. PMID: 30026537
    2. PI3K is a signal linker between L-selectin and PSGL-1 in IL-18 transcriptional activation at the promoter level. PMID: 29218606
    3. SELL expression was not altered in systemic sclerosis. PMID: 29356883
    4. Dual regulation of CD62L by HIV-1 via enhanced expression by Vpr in contrast with cell-surface down-modulation by Nef and Vpu has been reported. PMID: 30119013
    5. Recombinant human IL33 inhibited trophoblast invasion and adhesion, and decreased adhesion and invasionassociated molecules such as integrin alpha4beta1 and CD62L. PMID: 28765940
    6. CD62L has merit for risk stratification in natalizumab treatment is associated with progressive multifocal leukoencephalopathy. PMID: 26432858
    7. sLe(x) expressed on human L-selectin is preferentially bound by E-selectin and, on ligation, initiates secretion of MRP8/14 that binds TLR4 to elicit the extension of beta2-integrin to an intermediate affinity state. PMID: 28811304
    8. Here, we further developed and applied this method to express and purify the entire extracellular region of CD62L. This resulted in excess of 20 mg/L yield of recombinant CD62L. In an attempt to understand the different expression levels among four similar CD62L constructs that differ primarily in signal sequences, we calculated the presence of potential RNA pseudoknots in their signal sequences PMID: 28842197
    9. identified CD62L as a marker of a distinct NKT subset endowed with high proliferative potential and have developed artificial antigen-presenting cells that generate CD62L-enriched NKTs for effective cancer immunotherapy PMID: 27183388
    10. The -642C>T and 725C>T SELL polymorphisms are protective factors against acute coronary syndrome. SELL gene expression was increased in ACS patients. PMID: 28478085
    11. CD62L downregulation due to unconstrained HIV-1 replication may have important consequences for T-cell circulation and function and for HIV-1 disease progression PMID: 27003497
    12. found a higher frequency of LIN1(-) CCR3(+) eosinophils, and decreased expression of CD23 and CD62L receptors in eosinophils of AD patients PMID: 27406841
    13. Indian patients with primary Sjogren's syndrome have higher salivary sL-selectin and IL-7 levels than healthy controls PMID: 27620619
    14. While total surface expression of CD11b and L-selectin on neutrophils was largely unaffected PMID: 26361072
    15. These data confirm the expression of CD62L on urothelial carcinoma cells and suggest that CD62L may serve as biomarker to predict the presence of or risk for developing metastatic disease in patients with bladder cancer. PMID: 25618296
    16. freeze and thaw of murine and human Tregs is associated with reduced expression of L-selectin (CD62L), which was previously established to be an important factor that contributes to the in vivo protective effects of Tregs PMID: 26693907
    17. Modification of Levels of Adhesion Molecule Expression of Human Innate Immune Cells by Glycopolymers of Marine Bacteria PMID: 26852488
    18. Here we report that HIV-1 down-modulates CD62L in productively infected naive and memory resting CD4 T cells while suppressing Foxo1 activity and the expression of KLF2 mRNA. PMID: 25330112
    19. the polymorphisms within L-selectins gene were not associated with Visceral leishmaniasis and it may be concluded that these genotypes and alleles are unable to affect immune responses in Visceral leishmaniasis patients PMID: 25209910
    20. Functional analyses revealed that the lymphocyte homing receptor L-selectin (CD62L) is the key factor controlling the binding of chronic lymphocytic leukemia cells to high endothelial venule walls in vivo. PMID: 26162407
    21. SELL rs7531806 and rs1060573 are involved in androgen metabolism, inflammation processes and scar formation in severe acne. PMID: 24399259
    22. Both Nef and Vpu associated with and sequestered CD62L in perinuclear compartments, thereby impeding CD62L transport to the plasma membrane. PMID: 25822027
    23. Variants within the SELL gene are associated with sL-selectin levels. Despite accounting for a significant portion of the protein level variance, none of the variants was associated with clinical or subclinical cardiovascular disease. PMID: 25576479
    24. P-selectin glycoprotein ligand-1 and L-selectin have roles in neutrophil recruitment and activate human endothelial colony-forming cells at the site of vessel injury PMID: 24606340
    25. Increased expression of L-selectin ligands may be involved in the implantation process in tubal pregnancy. PMID: 24829027
    26. A cellular model system for quantifying L-selectin adhesion mechanics, is described. PMID: 23927766
    27. Individuals who undertake physical activity regularly, benefit from each single physical effort, because of decrease in serum concentration of proinflammatory molecules such as L-selectin and P-selectin. PMID: 25095634
    28. in a large, multiethnic population, soluble L-selectin levels did not predict clinical or subclinical cardiovascular disease PMID: 24631064
    29. results indicate a regulatory influence of MR signaling on human T-cell migration and suggest a role for endogenous aldosterone in the redistribution of T-cell subsets to lymph nodes, involving CD62L, CCR7, and CXCR4. PMID: 24595810
    30. the association between expression of the adhesion molecule CD62L (L-selectin) on naive and central memory T cells and the formation of antigen-specific antibodies differed significantly between younger and elder donors. PMID: 23571167
    31. The PSGL-1-L-selectin complex-induced signaling effects on neutrophil slow rolling and recruitment in vivo demonstrate the functional importance of this pathway. PMID: 24127491
    32. Molecular basis for the moesin/l-selectin/CaM ternary complex; an important role for phospholipids in modulating l-selectin function and shedding. PMID: 23796515
    33. the association of calmodulin with L-selectin PMID: 23658780
    34. Cell-based assessment of the percentage of L-selectin-expressing CD4 T cells could provide an urgently needed biomarker for individual risk assessment of progressive multifocal leukoencephalopathy (PML). PMID: 23925765
    35. Coronary artery disease patients blood samples showed a significantly higher L-selectin, but not CD11b response to TLR stimulation than controls. PMID: 23573259
    36. Over-activation of ADAM17 in NK cells may be detrimental to their effector functions by down-regulating surface expression of CD16 and CD62L. PMID: 23487023
    37. ATP induces CD62L downregulation from the surface of naive CD4+ T lymphocytes through the P2X7 receptor. PMID: 23319734
    38. association between L-selectin concentrations in plasma and skin damage in patients with systemic sclerosis PMID: 23028631
    39. Native glycodelin-A binds to peripheral blood monocytes inducing interleukin-6 secretion through L-selectin. PMID: 22977256
    40. determination of critical patch lengths of P-and L-selectin for the initiation of HL-60 cell binding in shear flow PMID: 22627390
    41. a unique lymphoid-primed population in human bone marrow was generated from hematopoietic stem cells before onset of expression of CD10 and commitment to the B cell lineage identified by high expression of the homing molecule L-selectin (CD62L). PMID: 22941246
    42. [review] Distinct domains of L-selectin contribute to proper leukocyte migration out of the vasculature into surrounding tissues during inflammation and immune surveillance. PMID: 21546114
    43. P213S polymorphism of the L-selectin gene is associated with type 2 diabetes and insulin resistance. PMID: 22921892
    44. Pretreatment of cardiac mesoangioblasts with SDF-1 or transient expression of L-selectin induced a two- to three-fold increase in their transmigration and homing to the damaged heart. PMID: 21869829
    45. No statistically significant results were found in order to sustain the hypothesis of association between SELL gene P213S polymorphism, type 2 diabetes mellitus and end stage renal disease. PMID: 22119815
    46. Levels of P-selectin and L-selectin were decreased in AD and lowest in AD patients with highest cognitive decline. Our findings suggest that these molecules may induce alterations of endothelial regulation and influence neurodegenerative processes of AD PMID: 21484243
    47. Lower frequency of CD62L(high) and higher frequency of TNFR2(+) Tregs are associated with inflammatory conditions in type 1 diabetic patients. PMID: 21584225
    48. L-selectin gene may play a role in the development of ischemic stroke. PMID: 21465128
    49. These results suggest that the association of CaM with L-selectin in the cell can be influenced by the membrane bilayer and that anionic lipids may modulate ectodomain shedding of transmembrane receptors. PMID: 21664913
    50. Data show that L-selectin TM and cytoplasmic domains lack the ability to dimerize in cell membranes. PMID: 21316337

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Selectin/LECAM family
  • 组织特异性：
    Expressed in B-cell lines and T-lymphocytes.
  • 数据库链接：

    HGNC: 10720

    OMIM: 153240

    KEGG: hsa:6402

    STRING: 9606.ENSP00000236147

    UniGene: Hs.728756