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  4. Human Ketohexokinase(KHK) ELISA kit

Human Ketohexokinase(KHK) ELISA kit

  • 中文名称：
    人己酮糖激酶(KHK)酶联免疫试剂盒
  • 货号：
    CSB-EL012157HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human KHK ELISA Kit was designed for the quantitative measurement of Human KHK protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 mU/mL-1200 mU/mL and the sensitivity is 4.68 mU/mL .

  • 别名：
    EC 2.7.1.3 ELISA Kit; Fructokinase ELISA Kit; Hepatic fructokinase ELISA Kit; Ketohexokinase ELISA Kit; Ketohexokinase isoform a ELISA Kit; KHK ELISA Kit; KHK_HUMAN ELISA Kit
  • 缩写：
    KHK
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    18.75 mU/mL-1200 mU/mL
  • 灵敏度：
    4.68 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human KHK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %84-94
    1:2Average %84
    Range %80-88
    1:4Average %95
    Range %90-99
    1:8Average %99
    Range %93-103
  • 回收率：
    The recovery of human KHK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-102
    EDTA plasma (n=4)9086-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    12002.547 2.461 2.504 2.352
    6002.143 2.076 2.110 1.958
    3001.403 1.449 1.426 1.274
    1500.814 0.765 0.790 0.638
    750.304 0.325 0.315 0.163
    37.50.226 0.218 0.222 0.070
    18.750.182 0.169 0.176 0.024
    00.156 0.147 0.152 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
  • 基因功能参考文献：
    1. Data indicate metabolic enzymes NAD kinase and ketohexokinase as candidate metabolic gene targets, and the chromatin remodeling protein INO80C as a tumor suppressor in KRAS(MUT) colorectal tumor xenograft. PMID: 28954733
    2. Angelica archangelica, Garcinia mangostana, Petroselinum crispum, and Scutellaria baicalensis were the top four botanical candidiates identified with inhibitory activity against ketohexokinase-C. PMID: 27322374
    3. compared with normal hepatocytes, hepatocellular carcinoma (HCC) cells markedly reduce the rate of fructose metabolism and the level of reactive oxygen species, as a result of a c-Myc-dependent and heterogeneous nuclear ribonucleoprotein (hnRNP) H1- and H2-mediated switch from expression of the high-activity fructokinase (KHK)-C to the low-activity KHK-A isoform. PMID: 27088854
    4. myocardial hypoxia actuates fructose metabolism in human and mouse models of pathological cardiac hypertrophy through hypoxia-inducible factor 1alpha (HIF1alpha) activation of SF3B1 and SF3B1-mediated splice switching of KHK-A to KHK-C PMID: 26083752
    5. These studies identify fructokinase as a novel mediator of diabetic nephropathy and document a novel role for endogenous fructose production, or fructoneogenesis, in driving renal disease. PMID: 24876114
    6. This study determined if single nucleotide polymorphisms in genes involved in fructose transport,SLC2A2 and SLC2A5 and metabolism, etohexokinase affect inter-individual variability in metabolic phenotypes. PMID: 23341889
    7. In human hepatocytes uric acid up-regulates KHK expression thus leading to the amplification of the lipogenic effects of fructose. PMID: 23112875
    8. ketohexokinase-A serves an unknown physiologic function that remains intact in essential fructosuria. PMID: 12941785
    9. The expression of ketohexokinase is diminished in human clear cell type of renal cell carcinoma PMID: 16372272
    10. Ketohexokinase-dependent metabolism of fructose induces proinflammatory mediators in proximal tubular cells. PMID: 19158351
    11. The structure of the KHK-A ternary complex revealed an active site with fructose & the ATP analogue in positions ready for phosphorylation. The effects of the pathogenic mutations Gly40Arg & Ala43Thr have been modelled in the context of the KHK structure. PMID: 19237742

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  • 相关疾病：
    Fructosuria (FRUCT)
  • 蛋白家族：
    Carbohydrate kinase PfkB family
  • 组织特异性：
    Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.
  • 数据库链接：

    HGNC: 6315

    OMIM: 229800

    KEGG: hsa:3795

    STRING: 9606.ENSP00000260598

    UniGene: Hs.567297