Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Interleukin 35(IL-35) ELISA Kit

Human Interleukin 35(IL-35) ELISA Kit

  • 中文名称：
    人白介素35(IL-35)酶联免疫试剂盒
  • 货号：
    CSB-E13126h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        IL-35（干扰素样21，IFN-λ5）是一种抑制性的细胞因子，属于IL-12家族。它由EBV转化的B细胞、Breg和Treg产生，具有调节和抑制细胞免疫功能的作用。IL-35被认为是抑制T细胞和B细胞活化的重要细胞因子，可以抑制Th17细胞的分化和功能。IL-35在某些自身免疫疾病和肿瘤的免疫治疗中显示出一定的治疗前景。
        j9九游会登录入口首页生物所提供的Human Interleukin 35(IL-35) ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、细胞培养上清、尿液、组织匀浆样本中的IL-35，其灵敏度为15.6 pg/mL，检测范围为62.5 pg/mL-4000 pg/mL。
     
  • 缩写：
    IL-35
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, cell culture supernates, urine, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-98  
    1:2 Average % 98  
    Range % 94-103  
    1:4 Average % 97  
    Range % 90-106  
    1:8 Average % 94  
    Range % 88-109  
  • 回收率：
    The recovery of human IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-97  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.872 2.752 2.812 2.705  
    2000 2.279 2.179 2.229 2.122  
    1000 1.545 1.589 1.567 1.460  
    500 0.952 1.002 0.977 0.870  
    250 0.531 0.575 0.553 0.446  
    125 0.341 0.366 0.354 0.247  
    62.5 0.255 0.275 0.265 0.158  
    0 0.108 0.105 0.107 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-35 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled IL-35 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

    显示更多

    收起更多

  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

    显示更多

    收起更多

  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

关于这个人白介素35(IL-35) ELISA Kit（CSB-E13126h）的特异性我有些疑问，请问检测抗体是专门检测IL-35还是IL-35的亚基（EBI-3和IL-12p35）？

A:
该试剂盒（CSB-E13126h）是用于检测IL-35化合物的。试剂盒中的两种抗体是专门为两个亚基设计的。

靶点详情

  • 最新研究进展：
    IL-35是一种由IL-12家族成员p35和EBI3亚单位组成的细胞因子，它具有免疫抑制和抗炎作用。近年来的研究表明，IL-35在炎症性和自身免疫性疾病中具有潜在的治疗作用。例如，在小鼠实验中，IL-35能够抑制自身免疫性疾病的发生，如自身免疫性脑脊髓炎、炎症性肠病等。此外，IL-35也被发现在某些肿瘤中表达水平较高，研究人员尝试利用其在肿瘤免疫治疗中的潜在作用。