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  4. Human Interleukin 32(IL-32) ELISA Kit

Human Interleukin 32(IL-32) ELISA Kit

  • 中文名称：
    人白介素32(IL-32)酶联免疫试剂盒
  • 货号：
    CSB-E12074h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human IL-32 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human IL-32 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the IL-32 levels in the sample and is measured at 450 nm through a microplate reader.

    IL-32 is a pro-inflammatory cytokine that is identified as an immunoregulator that controls cell differentiation and cell death and is involved in the stimulation of anti−/pro-inflammatory cytokines. It is inducibly expressed in response to cellular stress such as hypoxia, infections, and pro-inflammatory cytokines. It plays a crucial role in host defense against pathogens, as well as in the pathogenesis of chronic inflammation. Aberrant IL-32 expression has been associated with several autoimmune diseases such as rheumatoid arthritis, inflammatory bowel diseases, and type 1 diabetes, as well as allergic and infectious diseases, and cancer.

  • 别名：
    IL 32alpha ELISA Kit; IL 32beta ELISA Kit; IL 32delta ELISA Kit; IL 32gamma ELISA Kit; IL-32 ELISA Kit; IL32 ELISA Kit; IL32_HUMAN ELISA Kit; Interleukin 32 ELISA Kit; Interleukin 32 small ELISA Kit; Interleukin 32 theta ELISA Kit; Interleukin-32 ELISA Kit; interleukin-32 eta ELISA Kit; Natural killer cell transcript 4 ELISA Kit; Natural killer cells protein 4 ELISA Kit; NK 4 ELISA Kit; NK4 ELISA Kit; OTTHUMP00000236040 ELISA Kit; OTTHUMP00000236041 ELISA Kit; OTTHUMP00000236042 ELISA Kit; OTTHUMP00000236043 ELISA Kit; OTTHUMP00000236044 ELISA Kit; OTTHUMP00000236045 ELISA Kit; OTTHUMP00000236046 ELISA Kit; OTTHUMP00000236047 ELISA Kit; OTTHUMP00000236048 ELISA Kit; OTTHUMP00000236049 ELISA Kit; OTTHUMP00000236050 ELISA Kit; OTTHUMP00000236051 ELISA Kit; OTTHUMP00000236052 ELISA Kit; OTTHUMP00000236053 ELISA Kit; OTTHUMP00000236054 ELISA Kit; OTTHUMP00000241545 ELISA Kit; OTTHUMP00000241602 ELISA Kit; OTTHUMP00000241603 ELISA Kit; OTTHUMP00000241604 ELISA Kit; OTTHUMP00000241605 ELISA Kit; OTTHUMP00000241606 ELISA Kit; OTTHUMP00000241607 ELISA Kit; OTTHUMP00000241608 ELISA Kit; OTTHUMP00000241609 ELISA Kit; OTTHUMP00000241610 ELISA Kit; OTTHUMP00000241611 ELISA Kit; OTTHUMP00000241612 ELISA Kit; TAIF ELISA Kit; TAIFa ELISA Kit; TAIFb ELISA Kit; TAIFc ELISA Kit; TAIFd ELISA Kit; Tumor necrosis factor alpha-inducing factor ELISA Kit
  • 缩写：
    IL32
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    312.5 pg/mL-20000 pg/mL
  • 灵敏度：
    78.125 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-32 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 82-97  
    1:2 Average % 98  
    Range % 94-104  
    1:4 Average % 97  
    Range % 90-105  
    1:8 Average % 87  
    Range % 82-92  
  • 回收率：
    The recovery of human GROα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-97  
    EDTA plasma (n=4) 101 96-106  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    20000 2.801 2.752 2.777 2.627  
    10000 2.440 2.279 2.360 2.210  
    5000 1.784 1.789 1.787 1.637  
    2500 0.961 1.002 0.982 0.832  
    1250 0.547 0.575 0.561 0.411  
    625 0.338 0.346 0.342 0.192  
    312.5 0.234 0.225 0.230 0.080  
    0 0.146 0.154 0.150 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血浆（抗凝剂） EDTA

样品信息：人

稀释比：没有稀释

产品评价： ELISA结果正常组和患病组有明显差异

By 刘老师

靶点详情

  • 功能：
    Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.
  • 基因功能参考文献：
    1. IL-32gamma functions as intracellular effector in hepatocytes for suppressing hepatitis b virus replication. PMID: 30115930
    2. Results indicate that interleukin-32 gamma (IL-32gamma) plays a protective role for bone loss, providing clinical evidence of a negative correlation between IL-32gamma and DKK1 (Dickkopf-1) as bone metabolic markers. PMID: 28079119
    3. Our study shows a functional effect of a promoter single-nucleotide polymorphism (SNP) in IL32 on lipid profiles in RA patients and individuals, suggesting a possible protective role of this SNP against CVD. PMID: 28134327
    4. IL-32 may be an important pro-inflammatory molecule involved in calcific aortic valve disease. PMID: 29748157
    5. High IL32 expression is associated with hepatocellular carcinoma. PMID: 29286122
    6. Strong expression of IL-32 was detected in cardiomyocytes from heart failure patients. PMID: 28747035
    7. The increase in serum levels of IL-32 in accordance with additive effect of the presence of C allele in Multiple sclerosis patients might introduce IL-32 as a key player in MS pathogenesis or immunedysregulation PMID: 28716229
    8. high expression of both IL17A and IL32 leads to enhancement of T cell responses in breast tumors PMID: 28470472
    9. In transgenic mice, the presence of IL-32gamma contributes to the lesion healing caused by Leishmania braziliensis but not by Leishmania amazonensis. PMID: 28709468
    10. High IL32 expression is associated with lung metastasis in melanoma. PMID: 27589563
    11. In Kaposi sarcoma (KS) splicing ratio of the IL-32 isoforms showed IL-32gamma as the highest expressed isoform, followed by IL-32beta, in HIV-related KS cases compared with controls. Our data suggest a possible survival mechanism by the splicing of IL-32gamma to IL-32beta and also IL-6, IL-8, and CXCR1 signaling pathways to reverse the proapoptotic effect of the IL-32gamma isoform, leading to tumor cell survival and th... PMID: 29037857
    12. Three single nucleotide polymorphisms (SNPs) (rs28372698, rs12934561, rs4786370) of the IL32 gene have been proposed as modifiers for different diseases. The present study found no significant differences in the genotypic frequencies between the patients and healthy controls and no relation to survival for any of the SNPs. PMID: 29277790
    13. This study showed that the induction level of IL-32 was increased in chronic rhinosinusitis with nasal polyps compared to normal nasal mucosa and that LPS-induced IL-32 expression in nasal polyp-derived fibroblasts was regulated via the TLR4/JNK/AKT/CREB signaling pathway. PMID: 27173130
    14. findings show that IL-32alpha acts on NK cells to inhibit IL-15-mediated STAT5 phosphorylation and to suppress their IL-15-induced effector molecule expression and cytolytic capacity. IL-32alpha also acted on DCs by downregulating IL-15-induced IL-18 production, an important cytokine in NK cell activity. PMID: 28701509
    15. Thus, endogenous IL-32 is a crucial cytokine involved in the host defense against Leishmania parasites. PMID: 28241012
    16. Study indicated the overexpression of IL-32 in induced sputum of smokers and chronic obstructive pulmonary disease (COPD) patients, which was correlated with smoking exposure index and the degree of airway obstruction. It demonstrated that IL-32 might play critical role in smokers with COPD. PMID: 24761997
    17. this study shows that IL-32 might be involved in the pathogenesis of hepatic fibrosis by inducing TIMP-1 expression PMID: 27302771
    18. serum levels of IL-32 and TNF-alpha may be diagnostic markers, and serum IL-29 levels may be associated with good prognosis in patients with gastric cancer PMID: 26219901
    19. the importance of IL-32 polymorphism and mRNA expression in susceptibility and influence of survival status in lung cancer PMID: 27775437
    20. Data identify interleukin-32 (IL-32) as a potential therapeutic target. PMID: 26978598
    21. Low IL-32 expression is associated with colon cancer. PMID: 26824417
    22. Results show low plasma IL-32 levels in patients with systemic lupus erythematosus (SLE) and the rs28372698 SNP was associated with the susceptibility to SLE suggesting a possible role as a candidate marker to monitor SLE disease stability and screening in future. PMID: 27069296
    23. High IL32 expression is associated with acute myeloid leukemia. PMID: 26516703
    24. highlight some of the potential mechanisms by which the immunomodulatory effect of IL-32 occurs against mycobacterial infections but also areas where mechanistic clarifications are needed PMID: 26144292
    25. Additional IL-32gamma stimulation in precursor cells enhanced osteoblast differentiation potentially PMID: 26634249
    26. This study demonstrates that IL-32gamma and IL-32beta can induce caspase-8-dependent cell death whereas this was not observed for IL-32alpha PMID: 26678222
    27. High IL32 expression is associated with Sezary syndrome. PMID: 26551670
    28. soluble IL-6R expression upregulated the levels of its own ligand, IL-6 and those of the pro-inflammatory cytokine IL-32. PMID: 25176527
    29. Our results suggest that NKP30-B7-H6 interaction can aggravate hepatocyte damage, probably through up-regulation of IL-32 expression in hepatitis B virus-related acute-on-chronic liver failure PMID: 26241657
    30. These results suggest that IL-32beta could activate NF-kappaB and STAT3, and thus affect neuroinflammation as well as amyloidogenesis, leading to worsening memory impairment. PMID: 25159479
    31. IL-32 is involved in the pathogenesis of airway inflammation. PMID: 26151454
    32. High IL-32 expression may stimulate the organic metastasis and the lymph node metastasis of colorectal cancer. PMID: 25889282
    33. data further confirms that reduced IL32 methylation is associated with JIA, and that SNPs play an interactive role PMID: 26057774
    34. IL-32alpha can prevent cerebral ischemia damage via STAT3 activation and inhibition of NF-kappaB activation. PMID: 24854197
    35. In IL-32alpha-Tg mice, azoxymethane-induced colon cancer incidence was decreased, but expression of TNFR1 and TNFR1-mediated apoptosis was increased. IL-32alpha increased ROS production, prolonging JNK activation. In colon cancer patients, IL-32alpha was increased. PMID: 25909160
    36. IL-32 stimulation promotes the invasion and motility of osteosarcoma cells, possibly via the activation of AKT and the upregulation of MMP-13 expression. PMID: 25846944
    37. The anti-Mycobacterium tuberculosis effects of IL-32gamma are mediated through classical caspase-3-dependent apoptosis as well as caspase-3-independent apoptosis. PMID: 25887904
    38. IL-32 gamma delays, in a dose-dependent manner, the spontaneous apoptosis of human blood neutrophils by activating mainly p38 MAPK through rapid p38 phosphorylation. PMID: 25275312
    39. we demonstrate that the newly discovered isoform, IL-32theta;, suppresses monocyte differentiation by regulating the expression of the PU.1 transcription factor. PMID: 25726525
    40. This study demonstrates that IL-32 gene polymorphisms are significantly associated with increased endometrial cancer susceptibility in Chinese Han women PMID: 25663496
    41. These results suggest that IL-32gamma enhances an innate immune response against local infection but inhibits the spread of immune responses, leading to systemic immune disorder. PMID: 24743568
    42. Transgenic mice that express human interleukin-32 are protected from high-fat diet-induced hepatic steatosis. PMID: 25645248
    43. overexpression of IL-32gamma contributes to initial islet beta-cells injury and inflammation in pancreas and aggravates streptozotocin-induced type 1 diabetes. PMID: 25022955
    44. IL32 may play a unique role in mycosis fungoides progression as an autocrine cytokine PMID: 24938282
    45. Our results clearly suggest that IL-32 is an important mediator for gastric cancer metastasis and independent prognostic predictor of gastric cancer. PMID: 24602839
    46. The Epstein-Barr virus LMP1-induced IL-32 traps protein kinase Cdelta in the cytoplasm and prevents it from binding to the Zta promoter, which is the key event for virus activation. PMID: 25810549
    47. IL-32theta;, through its interaction with PKCdelta, downregulates CCL5 expression by mediating the phosphorylation of STAT3 on Ser727 to render it transcriptionally inactive. PMID: 25280942
    48. IL-32gamma enhances host immunity to Mycobacterium tuberculosis. PMID: 25820174
    49. IL-32alpha inhibits BCL6 SUMOylation by activating PKCepsilon, resulting in the modulation of BCL6 target genes and cellular functions of BCL6. PMID: 25245533
    50. IL-32gamma prevents ethanol-induced hepatic injury via the inhibition of oxidative damage and inflammatory responses. PMID: 25583360

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Selectively expressed in lymphocytes. Expression is more prominent in immune cells than in non-immune cells.
  • 数据库链接：

    HGNC: 16830

    OMIM: 606001

    KEGG: hsa:9235

    UniGene: Hs.943