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中文名称:人干扰素调节因子7(IRF7)酶联免疫试剂盒
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货号:CSB-EL011822HU
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:
This Human IRF7 ELISA Kit was designed for the quantitative measurement of Human IRF7 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.4 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.
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别名:IMD39 ELISA Kit; Interferon regulatory factor 7 ELISA Kit; Interferon regulatory factor 7H ELISA Kit; IRF 7 ELISA Kit; IRF 7A ELISA Kit; IRF 7H ELISA Kit; IRF-7 ELISA Kit; IRF7 ELISA Kit; IRF7_HUMAN ELISA Kit; IRF7A ELISA Kit; IRF7B ELISA Kit; IRF7C ELISA Kit; IRF7H ELISA Kit
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缩写:
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Uniprot No.:
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, tissue homogenates, cell lysates
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检测范围:23.4 pg/mL-1500 pg/mL
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灵敏度:5.8 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Immunology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human IRF7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 86-94 1:2 Average % 101 Range % 96-104 1:4 Average % 91 Range % 84-95 1:8 Average % 97 Range % 92-103 -
回收率:
The recovery of human IRF7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 87-97 EDTA plasma (n=4) 94 88-97 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1500 2.291 2.166 2.229 2.026 750 1.911 1.822 1.867 1.664 375 1.447 1.456 1.452 1.249 187.5 1.200 1.177 1.189 0.986 94 0.811 0.801 0.806 0.603 47 0.605 0.623 0.614 0.411 23.5 0.354 0.333 0.344 0.141 0 0.205 0.201 0.203 -
数据处理:
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货期:3-5 working days
引用文献
- Immunoregulatory factors (IRF3 and IRF7) serum levels and its relation to disease activity in Vitiligo patients M Anani,Journal of Pakistan Association of Dermatologists,2023
- Do Interferon Regulatory Factors 3 and 7 Play Role in Breast Cancer? SESA Mohsen,Egyptian Journal of Medical Microbiology,2020
- Nasal Fluid IRF7, CXCL9 and CXCL10 Protein Levels Do Not Reflect Altered Nasal Cell mRNA Expression Responses to Different Human Rhinovirus Species in Children with Asthma and Wheezing Exacerbations L.A. Coleman.et al,/,2018
相关产品
靶点详情
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功能:Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Induces transcription of ubiquitin hydrolase USP25 mRNA in response to lipopolysaccharide (LPS) or viral infection in a type I IFN-dependent manner. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.
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基因功能参考文献:
- cFLIP appears to bind to IKKalpha to prevent IKKalpha from phosphorylating and activating IRF7. PMID: 29222334
- our results suggest that expression of IRF7 is one of the metastatic effectors of LMP1 signalling in Epstein-Barr virus-associated nasopharyngeal cancer PMID: 28712115
- Hypomethylation of the IRF7 promoter might play a role in systemic sclerosis pathogenesis, probably through promoting the IRF7 expression in PBMCs of patients with SSc. PMID: 28952189
- Results showed that INF-lambda serum concentration was increased in Alzheimer's disease (AD) and mild cognitive impairment (MCI) carrying the IFNL3 T allele compared to healthy controls (HC). Anti-HSV-1 Ab titers were higher in AD and MCI individuals carrying the IRF7 AA genotype compared to HC. IFNL3 rs12979860 and IRF7 rs6598008 polymorphisms may modulate immune responses against HSV-1 via their effect on the IFN-lam... PMID: 28984602
- IRF7 plays a role in reducing bone metastasis of prostate cancer by IFN-beta-mediated NK activity. PMID: 27733217
- The MYC is shown to be recruited to the IRF7 promoter region through interaction with nuclear receptor corepressor 2/histone deacetylase 3 for its repression. PMID: 27630164
- S100A9 knockdown almost completely abrogated the effects of IRF7 deletion on granulocytic myeloid-derived suppressor cells (G-MDSC) development and tumor metastasis. IRF7 represents a novel regulator for G-MDSC development in cancer and may have predictive value for tumor progression. PMID: 28092673
- the transcription factor NFATC3 binds to IRF7 and functions synergistically to enhance IRF7-mediated IFN expression in Plasmacytoid dendritic cells. PMID: 27697837
- We show that IRF-7 siRNA knockdown enhanced LPS-induced IL-10 production in human monocyte-derived macrophages, and USP-18 overexpression attenuated LPS-induced production of IL-10 in RAW264.7 cells. Quantitative PCR confirmed upregulation of USP18, USP41, IL10, and IRF7. An independent cohort confirmed LPS induction of USP41 and IL10 genes PMID: 27434537
- KSHV-encoded viral IRF4 interacts with the host IRF7 and inhibits interferon-alpha production. PMID: 28342865
- The adaptor molecule RAIDD coordinates IKKepsilon and IRF7 interaction to ensure efficient expression of type I interferon. PMID: 27606466
- Bcl6, by interacting with the co-factors NcoR2 and HDAC3, plays a pivotal role in controlling IRF7 induction and antiviral signaling priming. PMID: 26728228
- Human IRF7 was shown to be essential for interferon type I-dependent protective immunity against primary influenza. (Review) PMID: 26761402
- The IRF7 GG genotype associate with Cognitive Decline and Dementia. PMID: 25835418
- IRF7 cleavage by the 3C protease of enterovirus D68 abrogated its capacity to activate type I interferon expression and limit virus replication. PMID: 26608321
- Interferon regulatory factor 7 (IRF7) is a direct target of miR-762 and overexpression of miR-762 reduced expression of IRF7. PMID: 26597380
- Data indicate that the type-I interferon master regulator gene interferon regulatory factor 7 (IRF7) is only hypomethylated in lupus patients with renal involvement. PMID: 26005050
- our results indicate that IRF7 promotes glioma cell invasion and both chemoresistance and radioresistance through AGO2 inhibition PMID: 25680411
- The interaction between AIP and IRF7 is enhanced upon virus infection, and AIP potently inhibits IRF7-induced type I IFN (IFN-alpha/beta) production. PMID: 25911105
- s found that knockdown of IRF7 leads to growth inhibition of Epstein-Barr virus-transformed cells, and restoration of IRF7 by exogenous plasmid correlates with growth recovery of the viral transformed cells. PMID: 25300801
- In TSC2-deficient angiomyolipoma patient cells, IRF7 is a pivotal factor in the Rheb/mTOR pathway. PMID: 25476905
- These findings suggest that IRF7-dependent amplification of type I and III IFNs is required for protection against primary infection by influenza virus in humans. PMID: 25814066
- s conclude that paramyxoviruses trigger the DNA damage response, a pathway required for MSK1 activation of phospho Ser 276 RelA formation to trigger the IRF7-RIG-I amplification loop necessary for mucosal interferon production. PMID: 25520509
- phosphorylation-mediated IRF7 transactivation is controlled by a tripod-helix structure. PMID: 25225665
- Data indicate that knockdown of IRF-7 expression almost completely diminished the enhancing effect of TLR9 signaling on Foxp3 expression, suggesting that IRF-7 is a downstream molecule of TLR9 signaling. PMID: 23490285
- In over 800 breast cancer patients, high expression of Irf7-regulated genes in primary tumors was associated with prolonged bone metastasis-free survival. PMID: 22820642
- Tat interaction with the 2 MAPKK and IRF7 promoters in HIV-1-infected cells and the resulting persistent activation of interferon-stimulated genes, which include inflammatory cytokines and chemokines, can contribute to the increased immune activation. PMID: 23535064
- In the present study, the s report that enterovirus 71 downregulates IRF7 through the 3C protein, which inhibits the function of IRF7. PMID: 23175366
- The major allele of a nonsynonymous polymorphism, rs1131665 (412Q) in IRF7, confers elevated activation of IRF-7 and predisposes to the development of systemic lupus erythematosus in multiple ethnic groups. PMID: 21360504
- None of the IRF7 polymorphisms was associated with systemic lupus erythematosus. PMID: 22433914
- phosphorylation of IRF7 on Ser477 and Ser479 by IKKepsilon or TBK1 is inhibited by KSHV ORF45 PMID: 22787218
- discuss the association of IRF7 and SLE based on recent understandings to render more information about the mechanisms of IRF7 might perform in PMID: 22455868
- Reconstruction of the wiring diagram of the modules revealed the presence of hyperconnected hub nodes, most notably interferon regulatory factor 7, which was identified as a major hub linking interferon-mediated antiviral responses. PMID: 22112518
- IRF7 single nucleotide polymorphism rs1061501 TT genotype and T allele are enriched in Taiwanese patients with systemic lupus erythematosus (SLE) and seem to be associated with an increased risk of developing SLE. PMID: 21632682
- Vesicular stomatitis Indiana virus-activated IRF7 upregulates expression of the BST2 gene independently of interferon signaling. PMID: 22301143
- TRAF6 can regulate HIV-1 production and expression of IRF7 promotes HIV-1 replication. PMID: 22140520
- IRF7 region is an anticentromere autoantibody propensity locus in systemic sclerosis PMID: 21926187
- Vaccinia virus protein C6 inhibits the activation of IRF7 by TBK1- and IKKepsilon-dependent pathways. PMID: 21931555
- The ubiquitin E3 ligase activity of tripartite motif-containing (TRIM)28 protein is specific to IRF7. PMID: 21940674
- Cigarette smoke suppresses key plasmacytoid dendritic cells functions upon viral infection by a mechanism that involves downregulation of TLR7 expression and decreased activation of IRF-7. PMID: 21435390
- Study did not find signi fi cant relationships between rs4963128 or rs2246614 of IRF7/KIAA1542 and the risk of systemic lupus erythematosus, differing from a previous study of European women PMID: 21167895
- analysis of IFN-stimulated response elements (ISREs) that bind to both the IFN-stimulated gene factor 3 (ISGF3) as well as to IFN response factor 7 (IRF7) PMID: 20943654
- ORF45 may maintain the IRF-7 molecule in the closed form and prevent it from being activated in response to viral infection PMID: 20980251
- These findings demonstrate that the lysine residues of IRF-7 play important roles in mediating IFN synthesis and modulating viral lytic replication. PMID: 20844090
- IRF-7-induced HPV8 transcription in primary keterinocytes. PMID: 20980500
- interferon regulatory factor 7C has dual roles in Epstein-Barr virus-mediated lymphocyte transformation PMID: 20209099
- HCV infection enhances STAT1 expression but impairs nuclear translocation of IRF-7 and its downstream molecules. These impairments in the IFN-alpha signaling pathway may, in part, be responsible for establishment of chronic HCV infection. PMID: 20810735
- Results suggest that Ro52-mediated ubiquitination promotes the degradation of IRF7 following TLR7 and TLR9 stimulation. PMID: 20668674
- EBV infection correlated with a blockage in the activation of JAK/STAT pathway members and affected the level of phosphorylated IFN regulatory factor 7 (IRF7). PMID: 20689596
- Smokers after infection with influenza is associated with reduced expression of IRF7 in nasal epithelial cells. PMID: 19880818
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相关疾病:Immunodeficiency 39 (IMD39)
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亚细胞定位:Nucleus. Cytoplasm. Note=The phosphorylated and active form accumulates selectively in the nucleus.
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蛋白家族:IRF family
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组织特异性:Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
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数据库链接:
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