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  4. Human Interferon epsilon(IFNE) ELISA kit

Human Interferon epsilon(IFNE) ELISA kit

  • 中文名称：
    人干扰素ε(IFNE)酶联免疫试剂盒
  • 货号：
    CSB-EL011049HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human IFNE ELISA Kit was designed for the quantitative measurement of Human IFNE protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    IFNE ELISA Kit; IFNE1 ELISA Kit; UNQ360/PRO655 ELISA Kit; Interferon epsilon ELISA Kit; IFN-epsilon ELISA Kit; Interferon epsilon-1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IFNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %85-95
    1:2Average %93
    Range %88-99
    1:4Average %92
    Range %87-100
    1:8Average %95
    Range %88-99
  • 回收率：
    The recovery of human IFNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9691-100
    EDTA plasma (n=4)9388-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.058 2.011 2.035 1.931
    5001.664 1.547 1.606 1.502
    2501.188 1.149 1.169 1.065
    1250.739 0.704 0.722 0.618
    62.50.561 0.521 0.541 0.437
    31.20.375 0.354 0.365 0.261
    15.60.219 0.211 0.215 0.111
    00.105 0.103 0.104 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Type I interferon required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. Directly mediates protection against viral and bacterial genital infections.
  • 基因功能参考文献：
    1. IFN epsilon protects macrophages against HIV infection through a type I IFN independent mechanism. PMID: 27942584
    2. human IFNvarepsilon suppresses HIV replication at multiple stages of infection. PMID: 28045025
    3. female sex workers decreased susceptibility to HIV-1 infection also have increased levels of IFNE gene and protein expression in the cervical epithelium PMID: 26555708
    4. T Allele of nonsense polymorphism (rs2039381, Gln71Stop) of interferon-epsilon is a risk factor for the development of intracerebral hemorrhage. PMID: 24055696
    5. Full-length IFN-epsilon 5'UTR markedly suppressed mRNA expression under basal conditions. It contains 2 stable stem-loop structures which associate with importin 9. Loop 1 is essential for regulation of mRNA expression. PMID: 23851686
    6. genetic polymorphism is related to onset time of vitiligo in Korean patients PMID: 23802172
    7. Observational study of gene-disease association. (HuGE Navigator) PMID: 20574843
    8. Observational study of gene-disease association. (HuGE Navigator) PMID: 20588308
    9. Observational study of gene-disease association. (HuGE Navigator) PMID: 20601674
    10. The structue and mRNA expression pattern of IFN-epsilon1 suggest that it may have a function distinct from those other members of type I INF. PMID: 15233997
    11. TNF-alpha leads to stabilization of IFN-epsilon mRNA, increased IFN-epsilon synthesis, engagement of type I IFNRs, increased STAT1 expression and phosphorylation, and up-regulation of retinoic acid-inducible gene-I expression PMID: 17878351
    12. A meta-analysis and a single-nucleotide polymorphism (SNP) rs1333049 representing the 9p21.3 locus provide unprecedented evidence for association between genetic variants at chromosome 9p21.3 and risk of coronary artery disease. PMID: 18362232
    13. In macrophages IFN-tau increased the synthesis of 2',5'-oligoadenylate synthetase/RNase L, MxA protein, IL-10 & IL-6, but not of IL-1ss or TNF-alpha. PMID: 18842358

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 组织特异性：
    Endometrium-specific.
  • 数据库链接：

    HGNC: 18163

    OMIM: 615223

    KEGG: hsa:338376

    STRING: 9606.ENSP00000418018

    UniGene: Hs.682604