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Human Interferon alpha-21(IFNA21) ELISA kit

  • 中文名称:
    人干扰素α-21(IFNA21)酶联免疫试剂盒
  • 货号:
    CSB-EL011039HU
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Human IFNA21 ELISA Kit was designed for the quantitative measurement of Human IFNA21 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名:
    IFNA21 ELISA Kit; Interferon alpha-21 ELISA Kit; IFN-alpha-21 ELISA Kit; Interferon alpha-F ELISA Kit; LeIF F ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    15.6 pg/mL-1000 pg/mL
  • 灵敏度:
    3.9 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human IFNA21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %98
    Range %91-101
    1:2Average %104
    Range %98-107
    1:4Average %87
    Range %83-91
    1:8Average %94
    Range %90-97
  • 回收率:
    The recovery of human IFNA21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9184-94
    EDTA plasma (n=4)10196-104
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.749 2.701 2.725 2.576
    5001.752 1.786 1.769 1.620
    2501.177 1.124 1.151 1.002
    1250.729 0.712 0.721 0.572
    62.50.509 0.487 0.498 0.349
    31.20.350 0.343 0.347 0.198
    15.60.256 0.249 0.253 0.104
    00.148 0.150 0.149
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    Alpha/beta interferon family
  • 数据库链接:

    HGNC: 5424

    OMIM: 147584

    KEGG: hsa:3452

    STRING: 9606.ENSP00000369574

    UniGene: Hs.113211