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  4. Human Integrin alpha-2(ITGA2) ELISA kit

Human Integrin alpha-2(ITGA2) ELISA kit

  • 中文名称：
    人整合素α2(ITGA2)酶联免疫试剂盒
  • 货号：
    CSB-EL011864HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ITGA2 ELISA Kit was designed for the quantitative measurement of Human ITGA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.235 ng/mL-15 ng/mL and the sensitivity is 0.058 ng/mL.

  • 别名：
    BR ELISA Kit; CD 49b ELISA Kit; CD49 antigen like family member B ELISA Kit; CD49 antigen-like family member B ELISA Kit; CD49b ELISA Kit; CD49b antigen ELISA Kit; Collagen receptor ELISA Kit; DX5 ELISA Kit; Glycoprotein Ia deficiency included ELISA Kit; GP Ia ELISA Kit; GP Ia deficiency; included ELISA Kit; GPIa ELISA Kit; HPA 5 included ELISA Kit; HPA5 included ELISA Kit; Human platelet alloantigen system 5 ELISA Kit; Integrin alpha 2 ELISA Kit; Integrin alpha II ELISA Kit; Integrin alpha-2 ELISA Kit; Integrin; alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) ELISA Kit; ITA2_HUMAN ELISA Kit; ITGA2 ELISA Kit; Platelet alloantigen Br(a); included ELISA Kit; Platelet antigen Br ELISA Kit; Platelet glycoprotein GPIa ELISA Kit; Platelet glycoprotein Ia ELISA Kit; Platelet glycoprotein Ia/IIa ELISA Kit; Platelet membrane glycoprotein Ia ELISA Kit; Platelet receptor for collagen; deficiency of; included ELISA Kit; Very late activation protein 2 receptor alpha 2 subunit ELISA Kit; VLA 2 alpha chain ELISA Kit; VLA 2 ELISA Kit; VLA 2 subunit alpha ELISA Kit; VLA-2 subunit alpha ELISA Kit; VLA2 ELISA Kit; VLA2 receptor alpha 2 subunit ELISA Kit; VLAA2 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.235 ng/mL-15 ng/mL
  • 灵敏度：
    0.058 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ITGA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:5 Average % 92
    Range % 88-96
    1:10 Average % 104
    Range % 100-107
    1:20 Average % 92
    Range % 89-96
    1:40 Average % 95
    Range % 93-97
  • 回收率：
    The recovery of human ITGA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 98 93-104
    EDTA plasma (n=4) 93 90-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    15 2.492 2.544 2.518 2.339
    7.5 2.150 2.231 2.191 2.012
    3.75 1.567 1.625 1.596 1.417
    1.88 0.944 0.955 0.950 0.771
    0.94 0.548 0.587 0.568 0.389
    0.47 0.353 0.352 0.353 0.174
    0.235 0.254 0.266 0.260 0.081
    0 0.177 0.180 0.179 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.; (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A.; (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.
  • 基因功能参考文献：
    1. Interleukin-4 induces a CD44high /CD49bhigh PC3 subpopulation with tumor-initiating characteristics PMID: 29236307
    2. Carriage of a combination of mutant alleles in multiple genes including ITGB3+CYP2C19*2 or CYP2C19*2 + ITGA2 or CYP2C19*2 are possible predictors of CVE in patients after CABG PMID: 29461866
    3. Studied the association between integrin subunit alpha 2 (GPIa) and prostaglandin-endoperoxide synthase 2 (COX-2) genetic polymorphisms in Chinese ishemic stroke patients with or without aspirin resistance. PMID: 28948649
    4. GP Ia C807T polymorphism may contribute to ischemic stroke development in the Chinese population (meta-analysis). PMID: 29208186
    5. The s identified a cell surface marker, ITGA2, which marks a proliferative trophoblast progenitor compartment in the first trimester placenta that is regulated by NOTCH signalling and exhibits unique expression characteristics. PMID: 29540503
    6. discovered that OLFM4, CD11b, and ITGA2 are proteins that are overexpressed in both primary colon tumors and liver metastasis. These are colon cancer biomarkers with autoantigenic properties. PMID: 28669815
    7. This study demonstrated that the transactivation of FOXL2 driven by interactions between HMGA2 and pRb might exert critical effects on the metastases and EMT of chemoresistant gastric cancer. Blocking the HMGA2-FOXL2-ITGA2 pathway could serve as a new strategy for gastric cancer treatment. PMID: 28119367
    8. The GpIa-C807T and GpIIIa-PlA1/PlA2 polymorphisms and more pronouncedly their combination are associated with increased risk of spontaneous abortions. PMID: 27405521
    9. The allele combination of CGC from hOGG1, ITGA2 and XPD polymorphisms was significantly associated with increased odds of nasopharyngeal carcinoma. PMID: 29121049
    10. blood platelets have a role in amyloid-beta aggregation in cerebral vessels through integrin alphaIIbbeta3-induced outside-in signaling and clusterin release PMID: 27221710
    11. Exposure to magnesium ions facilitated hBMSC proliferation via integrin alpha2 and alpha3 expression and partly promoted differentiation into osteoblasts via the alteration of ALP expression and activity PMID: 24616281
    12. The synonymous common variant, GPIA rs1126643, increases the long-term adverse events risk of CABG by augmenting GPIa protein expression and enhancing platelet aggregation function. PMID: 27881421
    13. we found a significant cumulative contribution of the genetic heterogeneity of glycoproteins Ia and IIIa and platelet-endothelial cell adhesion molecule-1 and P-Selectin genes in the risk of recurrent IVF-ET failures. The coexistence of these SNPs was associated with increased IVF-ET failure risk and the more polymorphic alleles or genotypes were present the higher the risk of IVF-ET failure, especially for younger women PMID: 28388872
    14. Meta-analysis found that glycoprotein Ia C807T T allele or the TT genotype, the Ser-allele of HPA-3 and B allele of glycoprotein Ibalpha variable number tandem repeat polymorphisms were associated with increased risk for ischemic stroke. PMID: 28004990
    15. Data indicate no association of maternal or fetal ITGA2 C807T SNP, ITGB3 T1565C SNP, PECAM1 CTG - GTG and SELP A/C polymorphisms with fetal growth restriction (FGR). PMID: 28358707
    16. The most common HPA genotypes among Saudis were HPA-1 a + b- (75%), HPA-2 a + b- (62%), HPA-3 a + b- (51.5%), HPA-4 a + b- (99%), HPA-5 a + b- (76.5%), HPA-6 a + b- (100%) and HPA-15 a + b + (50%). The prevalent allele among the HPA systems was (a), except in the HPA-15 system where the (b) allele was found in 52% of the subjects. PMID: 27019315
    17. A sulfonamide-type small-molecule inhibitor of alpha2beta1 integrin decreased average platelet adhesion in individuals with the C/T807T genotype but not in those harboring C807C. PMID: 26556301
    18. Platelet GP Ia 807C/T polymorphism could be a protective factor of for cerebral hemorrhage in the Chinese population. PMID: 26134877
    19. high miR-128 expression suppressed osteosarcoma cell migration, invasion, and EMT development through targeting ITGA2, which may be recommended as a therapeutic target for osteosarcoma. PMID: 26700675
    20. Study identified a novel RANKL function in the promotion of cell adhesion to type I collagen through the RANKL-NF-kappa B-integrin alpha 2 pathway and found integrin alpha2 and RANKL up-regulated in non small cell lung carcinoma. PMID: 27009236
    21. Recently, new clinical observations of genetic diseases provided evidence bringing new data on the role of alphaIIbbeta3 integrin in defective megakaryopoiesis. [review] PMID: 27011248
    22. High ITGA2 expression is associated with cervical cancer. PMID: 26758421
    23. PDI-mediated disulfide bond exchange plays a pivotal role in the post-ligation phase of alphaIIbbeta3-mediated adhesion to fibrinogen, while this step in alphavbeta3-mediated adhesion is independent of disulfide exchange PMID: 26514956
    24. Our results indicate that GpIa-807T and GpIIIa-PlA2 may be susceptibility alleles for in vitro fertilization implantation failure. PMID: 25643034
    25. PFL-mediated downregulation of integrin and EGFR contributes to the inhibition of tumor growth in vitro and in vivo. This novel anti-cancer mechanism of PFL suggests that this lectin would be useful as an anti-cancer drug or an adjuvant for other drugs. PMID: 26850110
    26. HRG binds to alpha2 integrin through low-affinity interactions in a heparin sulfate-independent manner, thereby blocking endothelial cells adhesion to collagen I. PMID: 26051322
    27. The Glycoprotein Ia C807T polymorphism might be a risk factor for the development and relapse of Acute Coronary Syndrome PMID: 26304618
    28. Data indicate that in recurrent spontaneous abortion patients, the expression levels of CD49b and LAG-3 (CD223) on CD14(+) mononuclear cells and the plasma level of transforming growth factor beta (TGF-beta) decreased obviously compared with normal females. PMID: 26927558
    29. Integrin alpha 2 and alpha 4 Yxxphi motifs interact with the endocytic clathrin adaptor, AP2 complex. PMID: 26779610
    30. Epigenetic silencing of ITGA2 by MiR-373 promotes cell migration in breast cancer. PMID: 26258411
    31. A highly bone metastatic RANKL-overexpressing prostate cancer cell line, LNCaP(RANKL)used as a model to study increased adhesion of prostate cancer (PCa) cells to collagens and found 3-D suspension culture and in vivo PCa tumor growth restore AR through downregulation of AP-4, enhancing integrin alpha2 expression and adhesion to ColI which is rich in bone matrices. PMID: 25200184
    32. diabetic retinopathy is associated with ITGA2 and ITGB3 PlA1/A2 polymorphisms (Meta-Analysis) PMID: 24979111
    33. This study shows that osteoblastic differentiation is improved on 3D scaffolds with submicron-scale texture and is mediated by integrin alpha2beta1. PMID: 25203434
    34. Current review discusses the mechanisms by which integrin is activated and subsequently deactivated by focusing on its structure-function relationship PMID: 26152212
    35. Polymorphism of HPA- 5 is probably a risk factor for carotid atherosclerosis complicating T2DM. PMID: 25966234
    36. blockade of alpha2beta1 integrin with a neutralizing mAb inhibited IL-7-induced bone loss and osteoclast numbers by reducing Th17 cell numbers in the bone marrow PMID: 26408663
    37. EZH2-silenced cells the signaling activity of the de-repressed ITGalpha2 is able to increase cofilin phosphorylation, which in turn reduces cell migration. PMID: 25549357
    38. The Integrin alpha2 2 gene C807T polymorphism may be a susceptible predictor of the risk of ischemic stroke. PMID: 24397542
    39. CD34 and CD49b are cell surface markers that can be used to enrich a subpopulation of leiomyoma cells possessing stem/progenitor cell properties. PMID: 25658015
    40. alpha2beta1 integrin gene expression levels were increased significantly in keloid fibroblasts under tension compared to normal fibroblasts under tension. Knockdown of Integrin A2B1 reduced cell proliferation in fibroblasts under tension. PMID: 25046655
    41. Carbohydrate-to-carbohydrate interactions between alpha2,3-linked sialic acids on alpha2 integrin subunits and asialo-GM1 underlie the bone metastatic behaviour of LNCAP-derivative C4-2B prostate cancer cells. PMID: 25137483
    42. The alphaII b tail sequences distal of GFFKR participate in talin-mediated inside-out alphaII b beta3 activation through its steric clashes with beta3 -bound talin PMID: 24837519
    43. The alpha2beta1 integrin mediates T-acute lymphoblastic leukemia cell migration through collagen in ERK and p38 MAPKs-dependent manner PMID: 24880062
    44. These results reveal betaig-h3 enhances the metastasis potentials of human osteosarcoma cells via integrin alpha2beta1-mediated PI3K/AKT signal pathways. PMID: 24595049
    45. ITGA2 is downregulated 39-fold by silencing of laeverin in trophoblasts, reducing cell migration and invasion. PMID: 24959655
    46. Molecular modeling indicated that galactosylation occurred on the periphery of alpha2beta1 integrin interaction with alpha1(IV)382-393 but right in the middle of alpha3beta1 integrin interaction with alpha1(IV)531-543 PMID: 24958723
    47. ITGA2 +807 gene polymorphism is not associated with susceptibility to CsA-induced GO. PMID: 24581850
    48. glycoprotein Ia C807T polymorphism is associated with idiopathic sudden hearing loss. PMID: 24466284
    49. It plays a role in regulation of leukocyte migration to a site of inflammation. PMID: 24850451
    50. Usage of internal N-acetylneuraminic acids and alpha2beta1 integrin by Wa rotavirus was at least partially additive. PMID: 24501414

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Integrin alpha chain family
  • 数据库链接：

    HGNC: 6137

    OMIM: 192974

    KEGG: hsa:3673

    STRING: 9606.ENSP00000296585

    UniGene: Hs.482077