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Human Integrin alpha-1(ITGA1) ELISA kit

  • 中文名称：
    人整合素α-1(ITGA1)酶联免疫试剂盒
  • 货号：
    CSB-EL011861HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ITGA1 ELISA Kit was designed for the quantitative measurement of Human ITGA1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    CD 49a ELISA Kit; CD49 antigen-like family member A ELISA Kit; CD49a ELISA Kit; CD49a antigen ELISA Kit; Integrin alpha-1 ELISA Kit; ITA1_HUMAN ELISA Kit; Itga 1 ELISA Kit; ITGA1 ELISA Kit; Laminin and collagen receptor ELISA Kit; Very late activation protein 1 ELISA Kit; VLA 1 ELISA Kit; VLA-1 ELISA Kit; VLA1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ITGA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 100
    Range % 100-105
    1:2 Average % 94
    Range % 89-98
    1:4 Average % 92
    Range % 87-95
    1:8 Average % 102
    Range % 94-107
  • 回收率：
    The recovery of human ITGA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 90-101
    EDTA plasma (n=4) 101 95-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 1.958 1.912 1.935 1.801
    10 1.442 1.467 1.455 1.321
    5 0.988 0.945 0.967 0.833
    2.5 0.562 0.551 0.557 0.423
    1.25 0.416 0.423 0.420 0.286
    0.625 0.303 0.309 0.306 0.172
    0.312 0.236 0.231 0.234 0.100
    0 0.133 0.135 0.134 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.
  • 基因功能参考文献：
    1. It was found that the levels of integrin alpha1 and VE-cadherin mRNA increased during co-culturing of activated endothelium cells with mesenchymal stromal cells. PMID: 29504106
    2. Results suggest that genetic variants in ITGA1 may be involved in the etiology of attention-deficit/hyperactivity disorder and the single-nucleotide polymorphisms -set based analysis is a promising strategy for the detection of underlying genetic risk factors. PMID: 28809852
    3. favors adhesive over migratory fibroblast phenotype PMID: 27294728
    4. Data suggest that the residue volume at phenylalanine (Phe) in alpha1-helix is critical for alpha(L)/beta(2) integrin (CD49a/CD18) activation and binding with soluble/immobilized ICAM1 (intercellular cell adhesion molecule 1). PMID: 29079572
    5. Studies suggest there are two nonoverlapping NK cell populations that are potentially liver resident in humans: CD49a+ NK cells and Eomes hi. PMID: 28318877
    6. ANGPTL1 inhibited angiogenesis by interacting with integrin alpha1beta1 receptor to suppress the downstreamJAK2-STAT3 signaling pathway. PMID: 28904065
    7. A range of affinities from almost no interaction to the relatively high avidity that characterizes alphaIIbbeta3 is seen between various alpha subunits and beta1 transmembrane and cytosolic domains. PMID: 27929375
    8. Taken together with our previous findings, we have replicated our results from the rodent system in a novel human system. We have revealed a unique sequential cascade involving Atg10, Wnt5a, alpha1 integrin, and matrix metalloproteinase-3 in GS/BMP-4-induced differentiation of hiPS cells into odontoblast-like cells at a relatively early stage. PMID: 27639333
    9. a comparative study between the wild type integrin alpha1 I and a gain-of-function E317A mutant. NMR HDX results suggest a relationship between regions exhibiting a reduced local stability in the unbound I domain and those that undergo significant conformational changes upon binding. PMID: 27342747
    10. MYC is a key regulating factor for the control of ITGA1 expression in colorectal cancer. PMID: 26096932
    11. Data indicate that CD49a(+) NK cells retain their phenotype after expansion in long-term in vitro cultures. PMID: 25672754
    12. Integrin alpha1 and beta4 are downregulated, following Dendrofalconerol A treatment, in H460 cells. PMID: 25550552
    13. miR-101:DNMT-3B interaction modulates integrin alpha-1 epigenetically. PMID: 24018042
    14. 17-beta-estradiol modulates connexins and integrins as well as ER-beta expression induced by high frequency electromagnetic fields. PMID: 23819010
    15. CD49a promoted Con A-induced hepatitis through enhancing inflammatory cytokine production (IFN-gamma and IL-17A) by CD4(+) T and invariant natural killer T cells. PMID: 24164540
    16. ITGA1 gene SNPs rs1862610, rs24321 43, and rs2447867 and the ITGA1 haplotype block that includes SNPs rs1862610 and rs2432143 were significantly associated with gastric cancer. PMID: 24124332
    17. small angle x-ray scattering data suggest that at low collagen peptide concentrations the complex exists in equilibrium between a 1:1 and 2:1 alpha1I-peptide complex. PMID: 24187131
    18. CagL is a versatile surface protein equipped with at least two motifs to promote binding to integrins, thereby causing aberrant signaling within host cells and facilitating translocation of CagA into host cells. PMID: 22919661
    19. Pro-inflammatory cytokine tumor necrosis factor (TNF)-alpha strongly promotes pericyte proliferation and migration, and concomitantly induces a switch in pericyte integrins, from alpha1 to alpha2 integrin. PMID: 23448258
    20. Discoidin domain receptors promote alpha1beta1- and alpha2beta1-integrin mediated cell adhesion to collagen by enhancing integrin activation. PMID: 23284937
    21. Collagen receptors alpha(1)beta(1) and alpha(2)beta(1) integrins are involved in transmigration of peripheral blood eosinophils, but not mononuclear cells through human microvascular endothelial cells monolayer PMID: 23070086
    22. Dynamic structural changes are observed upon collagen and metal ion binding to the integrin alpha1 I domain. PMID: 22847004
    23. study characterized the collagen binding properties of an activated variant of the alpha1I domain, harboring a gain-of-function mutation E317A; the activated alpha(1)I domain represents a novel conformation of the alphaI domain, mimicking the structural state where the Arg(287)-Glu(317) ion pair has just broken during the integrin activation PMID: 22030389
    24. we have identified a new locus candidate, ITGA1, influencing both fasting glucose and BMD, that may begin to explain the genetic contribution to the epidemiological observations linking type 2 diabetes and osteoporosis PMID: 22698912
    25. study identified integrin alpha1/beta1 and alpha2/beta1 heterodimer as a new candidate IgA1 receptor in human mesangial cells PMID: 22298882
    26. There was no evidence for differences in integrin alpha1, alpha4, beta1 and beta3 protein levels between the follicular and mid-luteal staged samples. PMID: 22002573
    27. the C-linker has a role as a spring-like element that allows relaxation of the alphaI domain in the resting state and controlled tension of the alphaI domain during activation, exerted by the beta chain PMID: 21965670
    28. alpha(1)beta(1) integrin is of importance not only for the differentiation of mesenchymal cells into myofibroblasts but also for the neovascularization and connective tissue organization PMID: 19397781
    29. Integrin alpha2beta1 might play a more crucial role in maintaining the mechanical creep properties of the collagen matrix than does integrin alpha1beta1. PMID: 21647271
    30. Streptococcus pyogenes M49 plasminogen/plasmin binding facilitates keratinocyte invasion via integrin-integrin-linked kinase (ILK) pathways and protects from macrophage killing PMID: 21521694
    31. Prostate tissue-derived CD133+ cells exhibited a moderate level of expression of alpha1 integrin PMID: 20531279
    32. Androgens increased INT alpha1 and alpha2 subunits in tubuloepithelial cells and in healthy labial salivary glands. PMID: 20436081
    33. Results show that plumieribetin, a fish lectin from the scorpionfish (Scorpaena plumieri), inhibits alpha1beta1 integrin binding to basement membrane collagen IV. PMID: 19850917
    34. results suggest that the inadequate trophoblastic invasion, induced by antiphospholipid antibodies, can be the result of decreased alpha1 integrin and VE-cadherin and increased alpha5 integrin and E-cadherin expression in the trophoblast PMID: 11937138
    35. role of VLA1 in migration of multiple sclerosis derived antigen-reactive T-cell migration PMID: 12078857
    36. determination of crystal structure in complex with an antibody Fab fragment PMID: 12662928
    37. endometrial integrin alpha1 and alpha4 expression is more consistently present in the early luteal phase in stimulated cycles than in natural cycles PMID: 12694973
    38. VEGF-A induced alpha1 & alpha2 integrins, promoting lymphatic endothelial tube formation & haptotactic migration. Lineage-specific integrin receptor expression contribute to the distinct dynamics of wound-associated angiogenesis & lymphangiogenesis. PMID: 15132990
    39. alpha(1)beta(1) and Col-IV contribute to beta-cell functions known to be important for islet morphogenesis and glucose homeostasis PMID: 15485856
    40. Enhanced expression of Integrin alpha1 is associated with liver metastases from gastrointestinal tumours PMID: 15679046
    41. analysis of binding between collagen type III and integrins alpha1beta1 and alpha2beta1 PMID: 16043429
    42. No statistical difference was observed on the expression of alpha1 integrin subunit expression in endometrium in tubal phimosis or hydrosalpinx PMID: 16412752
    43. identified integrin alpha1 as a protein tyrosine phosphatase type IVA-interacting protein for the first time, and verified this physical association with pull-down and co-immunoprecipitation assays PMID: 16472776
    44. alpha1beta1 integrin is a type IV collagen receptor in pancreatic cancer cells PMID: 17312461
    45. results define a crucial role for alpha1beta1 in controlling the accumulation of epidermal type 1 polarized effector memory T cells in a common human immunopathology and provide the basis for new strategies in psoriasis treatment PMID: 17603494
    46. thrombopoietin-induced in vitro differentiation of primary human cord blood mononuclear cells into megakaryocytes, we observed rapid, progressive CpG methylation of ITGA1, but not PELO or ITGA2. PMID: 17669516
    47. A significant subset of VLA-1-positive effector T cells from rheumatoid arthritis patients persists in vivo and in vitro during monoclonal antibody therapy and contributes to residual and recurring inflammation. PMID: 17891451
    48. are suggestive of the association of ITGA1 with osteoporosis and related risk in postmenopausal women PMID: 17931993
    49. Activation of the FAK-src molecular scaffolds and p130Cas-JNK signaling cascades by alpha1-integrins during colon cancer cell invasion. PMID: 17982677
    50. Group B streptococci ACP binds alpha(1)beta(1)-integrin via the D1 domain that promotes GBS internalization within epithelial cells. PMID: 18048918

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Integrin alpha chain family
  • 数据库链接：

    HGNC: 6134

    OMIM: 192968

    KEGG: hsa:3672

    STRING: 9606.ENSP00000282588

    UniGene: Hs.644352