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  4. Human Insulin-like peptide INSL5(INSL5) ELISA kit

Human Insulin-like peptide INSL5(INSL5) ELISA kit

  • 中文名称：
    人胰岛素样肽INSL5(INSL5)酶联免疫试剂盒
  • 货号：
    CSB-EL011749HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 别名：
    INSL5 ELISA Kit; INSL5_HUMAN ELISA Kit; Insulin like peptide INSL5 ELISA Kit; Insulin-like peptide 5 ELISA Kit; Insulin-like peptide INSL5 A chain ELISA Kit; MGC126695 ELISA Kit; MGC126697 ELISA Kit; PRO182 ELISA Kit; RIF2 ELISA Kit; RP24-72J16.1 ELISA Kit; UNQ156 ELISA Kit; UNQ156/PRO182 ELISA Kit
  • 缩写：
    INSL5
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    8 ng/mL-140 ng/mL
  • 灵敏度：
    5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human INSL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 85-103
    1:2 Average % 96
    Range % 89-105
    1:4 Average % 90
    Range % 86-97
    1:8 Average % 93
    Range % 91-98
  • 回收率：
    The recovery of human INSL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 90-102
    EDTA plasma (n=4) 94 88-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    140 1.586 1.597 1.592
    80 0.943 0.955 0.949
    32 0.365 0.371 0.368
    16 0.175 0.184 0.180
    8 0.101 0.105 0.103
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor.
  • 基因功能参考文献：
    1. Mass spectral analysis of purified enteroendocrine cells and tissue homogenates identified the exact sequence of A and B chains of INSL5 endogenously expressed in L-cells. PMID: 28857318
    2. analysis of the interaction mechanism of INSL5 with its receptor RXFP4 PMID: 28274616
    3. High serum INSL5 is associated with obesity in men. PMID: 27355242
    4. The C-terminus of the B-chain of human INSL5 is critical for cognate RXFP4 receptor activity. PMID: 26661035
    5. Insl5 is an orexigenic hormone released from colonic L-cells, which promotes appetite during conditions of energy deprivation. PMID: 25028498
    6. electrostatic interactions between INSL5 and RXFP4 PMID: 25043977
    7. High INSL5 expression is associated with enteroendocrine cells of the neuroendocrine tumors. PMID: 23128569
    8. INSL5 may be a unique marker of colorectal enteroendocrine cells, and INSL5-RXFP4 signaling might play a role in an autocrine/paracrine fashion in the colorectal epithelium and rectal neuroendocrine tumors. PMID: 23438439
    9. Insulin-like peptide 5 is an endogenous ligand for GPCR142 PMID: 15525639
    10. The hydrogen-bond network and electrostatic interactions between charged groups in INSL5 by NMR-monitored temperature and pH titrations was characterized. PMID: 19178384

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 组织特异性：
    Highly expressed in rectum with lower levels in uterus and ascending and descending colon.
  • 数据库链接：

    HGNC: 6088

    OMIM: 606413

    KEGG: hsa:10022

    STRING: 9606.ENSP00000302724

    UniGene: Hs.251380