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Human Immunoglobulin D(IgD) ELISA Kit

  • 中文名称:
    人免疫球蛋白D(IgD)酶联免疫试剂盒
  • 货号:
    CSB-E15051h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
        免疫球蛋白D(Immunoglobulin D,IgD)是人体中分布较少的一种免疫球蛋白,其主要存在于B淋巴细胞的表面,参与机体免疫应答的调节。IgD通过B细胞表面的抗原受体进行信号传递,促进B细胞的激活和增殖,是体液免疫和细胞免疫的重要组成部分。IgD的水平变化与多种疾病相关,如免疫系统异常、感染、过敏反应等。
        j9九游会登录入口首页生物所提供的Human Immunoglobulin D(IgD) ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的IgD,其灵敏度为0.024 ng/mL,检测范围为0.024 ng/mL-100 ng/mL。
     
  • 别名:
    N/A
  • 缩写:
    IgD
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.024 ng/mL-100 ng/mL
  • 灵敏度:
    0.024 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human IgD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 91  
    Range % 87-98  
    1:200 Average % 95  
    Range % 90-102  
    1:400 Average % 93  
    Range % 87-99  
    1:800 Average % 94  
    Range % 86-105  
  • 回收率:
    The recovery of human IgD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-96  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    100 2.120 2.094 2.107 2.005  
    25 1.655 1.637 1.646 1.544  
    6.25 0.995 1.005 1.000 0.898  
    1.56 0.515 0.507 0.511 0.409  
    0.39 0.413 0.408 0.411 0.309  
    0.1 0.204 0.210 0.207 0.105  
    0.024 0.138 0.132 0.135 0.033  
    0 0.100 0.103 0.102    
  • 数据处理:
  • 货期:
    3-5 working days

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靶点详情

  • 最新研究进展:
    IgD是一种抗体分子,也是B淋巴细胞表面的一种受体。它在机体的免疫防御中起着重要作用,通过与抗原结合,诱导B细胞的激活和增殖,促进免疫应答的发生。
    最近的研究表明,IgD在机体的免疫调节中具有重要作用。一项研究发现,在人类B细胞中,IgD能够与TLR7相互作用,从而抑制炎症介质的产生。另一项研究发现,在小鼠模型中,IgD能够抑制胰岛素样生长因子1(IGF1)的信号通路,从而降低肥胖相关的代谢疾病的发生率。