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Human Glypican-4(GPC4) ELISA kit

  • 中文名称：
    人磷脂酰肌醇聚糖4(GPC4)酶联免疫试剂盒
  • 货号：
    CSB-EL009706HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human GPC4 ELISA Kit was designed for the quantitative measurement of Human GPC4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    dJ900E8.1 glypican 4 ELISA Kit; Glypican proteoglycan 4 ELISA Kit; Gpc4 ELISA Kit; GPC4_HUMAN ELISA Kit; K Glypican ELISA Kit; K-glypican ELISA Kit; Kglypican ELISA Kit; OTTHUMP00000024052 ELISA Kit; Secreted glypican 4 ELISA Kit; Secreted glypican-4 ELISA Kit; UNQ474/PRO937 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GPC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 92-104  
    1:2 Average % 92  
    Range % 88-96  
    1:4 Average % 87  
    Range % 81-94  
    1:8 Average % 98  
    Range % 92-106  
  • 回收率：
    The recovery of human GPC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-96  
    EDTA plasma (n=4) 95 91-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.254 2.355 2.305 2.163  
    20 1.428 1.528 1.478 1.336  
    10 0.935 0.956 0.946 0.804  
    5 0.556 0.567 0.562 0.420  
    2.5 0.372 0.361 0.367 0.225  
    1.25 0.268 0.257 0.263 0.121  
    0.625 0.215 0.212 0.214 0.072  
    0 0.142 0.141 0.142 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system.
  • 基因功能参考文献：
    1. The T allele of GPC4 may represent a risk factor for Epstein-Barr virus-associated gastric carcinoma. PMID: 27071854
    2. According to behavioral studies, downregulation of Gpc4 by Gpc4 siRNA decreased spontaneous seizure frequency, while upregulation of Gpc4 by recombinant Gpc4 overexpression led to a converse result. These findings support the hypothesis that increased expression of Gpc4 in the brain is associated with epileptic seizures. PMID: 27425250
    3. serum level elevated in polycystic ovary syndrome and correlated with fat distribution and cardiovascular risk PMID: 26486309
    4. Data suggest gender difference in circulating GPC4 levels in nonalcoholic fatty liver disease; GPC4 levels in women appear to correlate with cardiometabolic risk factors (adiposity/body fat distribution, insulin resistance, and arterial stiffness). PMID: 23633195
    5. new function of miR-125a by targeting gene glypican-4 in cell growth process PMID: 22644326
    6. Findings establish that Gpc4 acts at the interface of extrinsic and intrinsic signal regulation to fine tune stem cell fate. PMID: 22761013

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.; [Secreted glypican-4]: Secreted, extracellular space.
  • 蛋白家族：
    Glypican family
  • 数据库链接：

    HGNC: 4452

    OMIM: 300168

    KEGG: hsa:2239

    STRING: 9606.ENSP00000359864

    UniGene: Hs.58367