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  4. Human Glycated hemoglobin A1c,GHbA1c ELISA Kit

Human Glycated hemoglobin A1c,GHbA1c ELISA Kit

  • 中文名称：
    人糖化血红蛋白A1c(GHbA1c)酶联免疫试剂盒
  • 货号：
    CSB-E08139h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human GHbA1c ELISA Kit was designed for the quantitative measurement of Human GHbA1c protein in lysates for RBC. It is a Competitive ELISA kit, its detection range is 0.012 ng/mL-50 ng/mL and the sensitivity is 0.012 ng/mL.

  • 缩写：
    GHbA1c
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    lysates for RBC
  • 检测范围：
    0.012 ng/mL-50 ng/mL
  • 灵敏度：
    0.012 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    50 0.270 0.273 0.272    
    12.5 0.367 0.378 0.373    
    3.125 0.463 0.489 0.476    
    0.78 0.560 0.598 0.579    
    0.195 0.692 0.672 0.682    
    0.049 1.384 1.368 1.376    
    0.012 1.994 1.954 1.974    
    0 2.373 2.399 2.386    
  • 本试剂盒所含材料：
    • A 96-well Assay plate --The 96-well plate has been pre-coated with GHbA1c antibody.
    • Standard (10 x concentrate) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • Biotin-conjugated GHbA1c (100 x concentrate) (1 x 60 μl)
    • HRP-avidin (100 x concentrate) (1 x 120 μl) --Catalyze the TMB solution, eliciting a chromogemic reaction.
    • Biotin-conjugate Diluent (1 x 10 ml) --Dilute the Biotin-conjugated GHbA1c
    • HRP-avidin Diluent (1 x 20 ml) --Dilute the HRP-avidin
    • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
    • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 客户评论
  • Sample type: Whole blood (Dried Blood Spot)
    Sample species: Human
    Review: ELISA kit works very well. Gives reproducible values between different lots.