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Human Fibrillin-3(FBN3) ELISA kit

  • 中文名称：
    人原纤维蛋白-3(FBN3)酶联免疫试剂盒
  • 货号：
    CSB-EL008458HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human FBN3 ELISA Kit was designed for the quantitative measurement of Human FBN3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

  • 别名：
    FBN3 ELISA Kit; KIAA1776Fibrillin-3 [Cleaved into: Fibrillin-3 C-terminal peptide] ELISA Kit
  • 缩写：
    FBN3
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human FBN3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %93-99
    1:2Average %94
    Range %89-98
    1:4Average %91
    Range %88-98
    1:8Average %86
    Range %80-96
  • 回收率：
    The recovery of human FBN3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8781-93
    EDTA plasma (n=4)9895-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.409 2.456 2.433 2.325
    101.535 1.585 1.560 1.452
    50.812 0.801 0.807 0.699
    2.50.506 0.545 0.526 0.418
    1.250.272 0.265 0.269 0.161
    0.6250.166 0.155 0.161 0.053
    0.3120.150 0.158 0.154 0.046
    00.104 0.111 0.108 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-containing microfibrils provide long-term force bearing structural support.
  • 基因功能参考文献：
    1. an association study showed a potential association of the D19S884 marker with PCOS in Chinese Han women and the meta-analysis identified that the A8 allele may increase susceptibility to PCOS PMID: 23265956
    2. The FBN3 risk allele may be associated with changes in basal glucose homeostasis in PCOS. PMID: 22301903
    3. Data show that TGF-beta pathways operate during ovarian fetal development, and fibrillin 3 is highly expressed at a critical stage early in developing human and bovine fetal ovaries. PMID: 21411746
    4. fibrillin-3, compared to the other fibrillins, fulfills both overlapping and distinct functions in human development PMID: 20970500
    5. A variant in the fibrillin-3 gene is associated with TGF-beta and inhibin B levels in women with polycystic ovary syndrome. PMID: 20630504
    6. Loss of fibrillin-3 during folliculogenesis may be an important factor in polycystic ovary syndrome pathogenesis. PMID: 20855553
    7. Single nucleotide polymorphisms in FBN3 occur and may be a candidate gene for polycystic ovary syndrome. PMID: 20200332
    8. Data show that fibrillin 3 appears to have little involvement in PCOS but cannot rule out that other markers in the region of chromosome 19p13.2 are associated with PCOS or that FBN3 expression occurs in other organs that may influence the PCOS phenotype. PMID: 19692420
    9. fibrillin-3 gene showed the strongest evidence for association with Polycystic ovary syndrome PMID: 17785364

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Fibrillin family
  • 组织特异性：
    Predominantly expressed in connective tissues such as skeletal muscle, tendon, skin, perichondrium and periosteum. Highly expressed in fetal lung, brain, kidney. Expressed at low level in prostate, testis, mammary gland, uterus, ovary, placenta, bladder,
  • 数据库链接：

    HGNC: 18794

    OMIM: 608529

    KEGG: hsa:84467

    STRING: 9606.ENSP00000270509

    UniGene: Hs.370362