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  4. Human Ferritin light chain(FTL) ELISA kit

Human Ferritin light chain(FTL) ELISA kit

  • 中文名称：
    人铁蛋白轻链(FTL)酶联免疫试剂盒
  • 货号：
    CSB-EL009049HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human FTL ELISA Kit was designed for the quantitative measurement of Human FTL protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

  • 别名：
    Ferritin L chain ELISA Kit; Ferritin L subunit ELISA Kit; Ferritin light chain ELISA Kit; Ferritin light polypeptide ELISA Kit; ferritin light polypeptide like 3 ELISA Kit; FRIL_HUMAN ELISA Kit; FTL ELISA Kit; LFTD ELISA Kit; NBIA 3 ELISA Kit; NBIA3 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human FTL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %87-95
    1:2Average %94
    Range %90-98
    1:4Average %94
    Range %89-97
    1:8Average %100
    Range %93-103
  • 回收率：
    The recovery of human FTL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9790-104
    EDTA plasma (n=4)9389-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    403.055 3.167 3.111 2.987
    202.389 2.288 2.339 2.215
    101.413 1.429 1.421 1.297
    50.857 0.842 0.850 0.726
    2.50.462 0.483 0.473 0.349
    1.250.320 0.334 0.327 0.203
    0.6250.245 0.251 0.248 0.124
    00.122 0.126 0.124 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • 基因功能参考文献：
    1. Mossbauer spectra of human liver ferritin and its pharmaceutical analogues Ferrum Lek and Maltofer(R) measured at various temperatures, are reported. PMID: 27372204
    2. Determining serum ferritin is a convenient and nonexpensive method to determine the outcome of the treatment of the cases with oral squamous cell carcinoma . Its potential as prognostic marker could not be overlooked PMID: 28862225
    3. The functional significance of the observed patch of carboxylate side chains and resulting metallocluster for biomineralization emerges from the lower iron oxidation rate measured in the E60AE61AE64A variant of human L-ferritin, leading to the proposal that the observed metallocluster corresponds to the suggested, but yet unobserved, nucleation site of L-ferritin PMID: 28202724
    4. The clinical measurement of ferritin in cerebrospinal fluid is a better biomarker than serum levels of ferritin for diagnosing and assessing the progression of amyotrophic lateral sclerosis patients. PMID: 27804118
    5. Our patient's transferrin saturation was 27% and HFE analysis revealed that she had neither H63D nor C282Y mutations that are known to predispose to hemochromatosis. Besides being an iron storage protein, ferritin is also one of the so-called acute phase proteins PMID: 28636169
    6. This is the first Australian report of the c.-167 C>T mutation in a large family with multiple affected individuals. PMID: 27096259
    7. Baseline serum ferritin (SF) did not influence bloodstream infections (BSIs), but higher levels resulted in more invasive fungal infections (IFIs) PMID: 28585071
    8. High serum ferritin expression is associated with metabolic syndrome. PMID: 27390880
    9. Hepatitis E virus ORF1 encoded macro domain protein interacts with light chain subunit of human ferritin and inhibits its secretion. PMID: 27170377
    10. indicate an important role of ferritin light chains in neurodegeneration PMID: 26994418
    11. This study demostrated that FTL mutation progressive brain iron dysregulation, morphological signs of early neurodegeneration and motor coordination deficits show PMID: 25447222
    12. FTL expression was higher in glioblastoma than in low-grade glioma, and decreased expression of FTL correlated with increased survival in glioblastoma patients. PMID: 26871431
    13. FTL gene mutation and persistent hyperferritinemia without iron deficiency anemia after phlebotomy PMID: 25720123
    14. Ferritin plasma levels increased significantly following stem cell transplantation in graft rejection patients. PMID: 26611853
    15. Single nucleotide polymorphisms in HAMP, BMP2, FTL and SLC40A1 genes have phenotype-modifying roles in hereditary hemochromatosis type 1. PMID: 25976471
    16. Genome-wide association study identifies variants in PMS1 associated with serum ferritin in a Chinese population. PMID: 25162662
    17. Urine ferritin levels are elevated significantly in systemic lupus erythematosus and correlate with disease activity. PMID: 22871034
    18. findings expand the genetic and clinical diversity of neuroferritinopathy and suggest CSF ferritin levels as a novel potential biochemical marker for the diagnosis of neuroferritinopathy. PMID: 24825732
    19. Plasma hepcidin-25 and ferritin light chain levels correlate with a malignant breast cancer diagnosis. PMID: 24306042
    20. provide a new mechanism for selective autophagy of ferritin and reveal a previously unappreciated role for autophagy and NCOA4 in the control of iron homeostasis in vivo PMID: 25327288
    21. Genetic testing confirmed the diagnosis of hereditary hyperferritinemia cataract syndrome (HHCS), demonstrating a C39>G (c.-161C>G) mutation into FTL gene. PMID: 24983587
    22. A c.-171C>G mutation in the iron-regulatory element of FTL was found in 2 members of a Spanish family with hyperferritinemia-cataract syndrome. PMID: 24022025
    23. Elevated cerebrospinal ferritin reliably (but unspecifically) indicates severe central nervous system disease. PMID: 24821637
    24. Through combining serum ferritin and MS spectral data, the diagnosis sensitivity and specificity of our model for prewarning severe aGVHD (III~IV degrees aGVHD) before transplant all increased to 90.0% PMID: 24195075
    25. Increased levels of ferritin light chain protein is associated with breast cancer. PMID: 23969999
    26. Together, our results suggest that iron can increase gamma-secretase activity through promoting the level of FTL that interacts with and stabilizes PEN-2 PMID: 23685131
    27. Elevated levels of ferritin are associated with type 2 diabetes mellitus. PMID: 23381919
    28. Data show the transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferases PRMT1 and PRMT4. PMID: 23699174
    29. Noncoding G-to-T transversion (c.-168G>T) located in the iron response element (IRE) of the gene coding for ferritin light chain (FTL) cosegregated with cataract in the family. PMID: 23592921
    30. The Badalona 36C > U and Heidelberg 52 G > C mutations within the L-ferritin Iron-Responsive Element only mildly alter the binding capacity of the Iron Regulatory Proteins but are still causative for hyperferritinaemia cataract syndrome. PMID: 23421845
    31. Elevation in ferritin is associated with response to trastuzumab in breast cancer. PMID: 23300545
    32. Plasma levels of FLT and S100A9 proteins are up-regulated and CNDP1 levels are down-regulated in patients with glioblastoma. PMID: 23029420
    33. data demonstrate an enhanced propensity of mutant ferritin to undergo iron-catalyzed oxidative damage and support this as a mechanism causing disruption of ferritin structure and iron mishandling that contribute to pathology of hereditary ferritinopathy PMID: 22348978
    34. Two novel missense L-ferritin variants are associated with hyperglycosylation, p.Gln26Ile and p.Ala27Val, and with benign hyperferritinemia in two unrelated patients. PMID: 22535864
    35. High ferritin is associated with poor treatment response in hematological neoplasms. PMID: 22248276
    36. The data strongly suggest that FTL and SCCA1 may serve as coreceptors in HBV cellular attachment and virus entry into hepatocytes. PMID: 22359459
    37. Molecular genetic analysis revealed point mutations within the FTL IRE. PMID: 22020773
    38. genetic variations in the HFE gene, but not plasma ferritin may have a role in coronary heart disease in Chinese PMID: 21696736
    39. Genetic analysis revealed mutation G32A in Pedigree 1 and mutation G32T in Pedigree 2, both heterozygous and located in the iron-responsive element of the ferritin light chain mRNA in hyperferritinemia cataract syndrome. PMID: 21907119
    40. FTL is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
    41. In the family with hyperferritinemia cataract syndrome a G-->C heterozygous mutation at position +32 of FTL was identified. PMID: 21541272
    42. Somatic mutations in the iron response elements (IRE) of the L-ferritin gene are infrequent in the age-related cataract. PMID: 21139976
    43. This protein has been found differentially expressed in thalami from patients with schizophrenia. PMID: 20471030
    44. Toluene diisocyanate (TDI) regulates haem oxygenase-1/ferritin expression: implications for toluene diisocyanate-induced asthma. PMID: 20345975
    45. biochemical and crystallographic characterization of pathogenic FTL mutant p.Phe167SerfsX26 showing that it is a functional ferritin with an altered conformation of the C terminus PMID: 20159981
    46. This protein has been found differentially expressed in the anterior cingulate cortex from patients with schizophrenia PMID: 20381070
    47. indicate that cellular iron imbalance and oxidative damage produced by the over-expression in of two pathogenic L-ferritin variants are primary causes of cell death, while aggregate formation is a secondary effect PMID: 19781644
    48. the x-ray crystallographic structure and report functional studies of ferritin homopolymers formed from the mutant FTL polypeptide PMID: 19923220
    49. Finding not only supports direct evidence for a regulatory role of L-ferritin in neuroectodermal cell pigmentation but also integrates a new player within a complicated network governing iron homeostasis in the dopamine neurons of substantia nigra. PMID: 19318681
    50. The genetic defects in the FTL gene are unlikely to be a common cause of typical PD, at least in a North America population. PMID: 12459518

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  • 相关疾病：
    Hyperferritinemia with or without cataract (HRFTC); Neurodegeneration with brain iron accumulation 3 (NBIA3); L-ferritin deficiency (LFTD)
  • 蛋白家族：
    Ferritin family
  • 数据库链接：

    HGNC: 3999

    OMIM: 134790

    KEGG: hsa:2512

    STRING: 9606.ENSP00000366525

    UniGene: Hs.433670