Human Estradiol,E2 ELISA Kit
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中文名称:
人雌二醇(E2)酶联免疫试剂盒
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货号:
CSB-E05108h
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规格:
96T/48T
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价格:
¥3200/¥2500
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其他:
产品详情
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产品描述:
雌二醇(Estradiol,E2)是一种雌激素,是雌性生殖系统发育和功能维持的重要激素。E2通过与细胞内的雌激素受体结合,调节多种生理过程,如生殖器官发育、月经周期、妊娠、乳腺发育等。除了生殖系统,E2还参与了神经、心血管、骨骼等系统的生理调节。E2在体内的水平受到多种因素的影响,如年龄、饮食、运动等。E2水平异常与多种疾病相关,如不孕、乳腺癌、骨质疏松等。E2的生理和病理作用受到广泛关注,E2也被用于多种药物的研发和治疗。 j9九游会登录入口首页生物所提供的Human Estradiol,E2 ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测人血清、血浆样本中的E2。
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别名:
N/A
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缩写:
E2
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种属:
Homo sapiens (Human)
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样本类型:
serum, plasma.
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检测范围:
50 pg/ml-1200 pg/ml.
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灵敏度:
40 pg/ml.
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反应时间:
1-5h
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样本体积:
50-100ul
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检测波长:
450 nm
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研究领域:
Signal Transduction
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测定原理:
quantitative
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测定方法:
Competitive
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本试剂盒所含材料:
- A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit E2 antibody.
- Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- Anti-mouse E2 Antibody 1 x 6 ml -- Act as the detection antibody.
- HRP-conjugate 1 x 6 ml -- Bind to the E2 antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
- Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
- Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
- Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
- Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
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本试剂盒不含材料:
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理:
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货期:
3-5 working days
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Estrogen is an important mediator of mast cell activation in ovarian endometriomas
Zhu T.et al,Reproduction.,2017
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Highly Sensitive Detection of Hormone Estradiol E2 Using Surface-Enhanced Raman Scattering Based Immunoassays for the Clinical Diagnosis of Precocious Puberty
Rui Wang.et al,ACS applied materials & interfaces,2016
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Yuehan Zhao, Yongdong Zhang, Fang Pan,Centr Eur J Immunol ,2015
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Retinoic acid has the potential to suppress endometriosis development
Yamagata Y. et al,J Ovarian Res,2015
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