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  4. Human Eotaxin 2/CCL24 ELISA Kit

Human Eotaxin 2/CCL24 ELISA Kit

  • 中文名称：
    人嗜酸粒细胞趋化蛋白Eotaxin 2(Eotaxin 2/CCL24)酶联免疫试剂盒
  • 货号：
    CSB-E10360h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        CCL24，也称为炎性细胞趋化蛋白-2（MIP-2），是C-C趋化因子家族中的一种趋化因子。它主要由树突状细胞、纤维细胞和肌肉细胞等产生，并且能够吸引CCR3受体表达的细胞，如嗜酸性粒细胞和嗜碱性粒细胞等。CCL24在过敏性疾病、哮喘和皮肤炎症等多种疾病中发挥着重要作用。
        j9九游会登录入口首页生物所提供的Human Eotaxin 2/CCL24 ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的CCL24，其灵敏度为67.660 pg/mL，检测范围为78.125 pg/mL-5000 pg/mL。
     
  • 别名：
    C C motif chemokine 24 ELISA Kit; C-C motif chemokine 24 ELISA Kit; CCL24 ELISA Kit; CCL24_HUMAN ELISA Kit; Chemokine CC Motif Ligand 24 ELISA Kit; CK beta 6 ELISA Kit; CK-beta-6 ELISA Kit; Ckb6 ELISA Kit; Eosinophil chemotactic protein 2 ELISA Kit; Eotaxin-2 ELISA Kit; MPIF 2 ELISA Kit; MPIF-2 ELISA Kit; MPIF2 ELISA Kit; Myeloid progenitor inhibitory factor 2 ELISA Kit; SCYA24 ELISA Kit; Small inducible cytokine A24 ELISA Kit; Small inducible cytokine subfamily A (Cys-Cys) member 24 ELISA Kit; Small-inducible cytokine A24 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78.125 pg/mL-5000 pg/mL
  • 灵敏度：
    67.660 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 95    
    Range % 90-100    
    1:2 Average % 89    
    Range % 85-94    
    1:4 Average % 96    
    Range % 91-101    
    1:8 Average % 104    
    Range % 100-108    
  • 回收率：
    The recovery of human eotaxin 2/CCL24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 99 95-103    
    EDTA plasma (n=4) 86 83-89    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    5000 2.152 2.204 2.178 2.065    
    2500 1.796 1.735 1.766 1.653    
    1250 1.182 1.205 1.194 1.081    
    625 0.765 0.798 0.782 0.669    
    312.5 0.453 0.468 0.461 0.348    
    156.25 0.328 0.331 0.330 0.217    
    78.125 0.251 0.240 0.246 0.133    
    0 0.115 0.110 0.113 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
        CCL24是一种细胞因子，主要由T淋巴细胞和单核细胞产生，可通过调节免疫细胞的迁移和激活来参与多种疾病的发生和发展。近年来的研究表明，CCL24在哮喘和过敏性鼻炎中发挥重要作用，并与疾病的病理生理过程密切相关。
     
  • 功能：
    Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
  • 基因功能参考文献：
    1. Study showed that CCL24 was upregulated in hepatocellular carcinoma (HCC) tissues and correlated with poor prognosis in HCC patients . Also, CCL24 was associated with the metastatic potential of HCC cell lines and promoted proliferation, migration, and invasion. PMID: 28042950
    2. Mean eotaxin 2 concentrations in nasal fluid in patients with perennial allergic rhinitis and nonallergic and allergic chronic rhinosinusitis with nasal polyps patients were significantly higher in comparison to control subjects. PMID: 28587510
    3. HMGB1 did not elicit chemotaxis of human eosinophils alone and had no effect in combination with the eosinophil chemotactic agent, eotaxin-2 (CCL24). PMID: 25774667
    4. these data suggest that CCL26 and CCL24 are likely involved in the pathogenesis of chronic nasal hypereosinophilia, with a complex cooperation and different involvement of the various members of eotaxin family. PMID: 24989688
    5. Pregnancy associated environments increased local CCL24/CCR3, supporting the process of decidualization in human early pregnancy. PMID: 23696919
    6. Phosphodiesterase 4 inhibitors, rolipram and RO-20-1724 have no effect on CCL24 expression in human primary bronchial epithelial cells. PMID: 22946025
    7. Study shows that CCL24 levels were significantly increased in age-related macular degeneration (AMD) patients despite Avastin treatment as compared with normal controls and those without Avastin. PMID: 23025269
    8. CCR3 is differentially expressed on inflammatory cells in rheumatoid arthritis, while eotaxin-2, a potent CCR3 agonist, is differentially expressed in active disease. PMID: 20659406
    9. stimulates lung fibroblast proliferation and collagen synthesis PMID: 20143648
    10. alters eosinophil integrin function via mitogen-activated protein kinases PMID: 12034562
    11. Intradermal injection of CCL24 induces recruitment of eosinophils, basophils, neutrophils, and macrophages as well as features of early- and late-phase allergic reactions in atopic and nonatopic volunteers. PMID: 12193745
    12. Plasma eotaxin-2 concentrations differed in asthmatic patients with respect to aspirin intolerance and tolerance, indicating that eotaxin-2 may be differentially up-regulated according to aspirin intolerance. PMID: 16304252
    13. Our results suggest that Eo2 +179T>C and Eo2 +275C>T of eotaxin-2 might be associated with the susceptibility of ulcerative colitis. PMID: 16391516
    14. These data support the idea that CCL24/eotaxin-2 is part of the mechanism of CD4 lymphocyte activation paracrinally induced by Nef. PMID: 17630924
    15. eotaxin-2 is a chemokine strongly associated with primary and metastatic tumors of colorectal origin PMID: 17908961
    16. the ability to produce eotaxin-2/CCL24 is acquired during the differentiation into eosinophilic lineage which is dependent on GATA-1 expression PMID: 17917245
    17. This protein is an eosinophil chemoattractant that is up-regulated by human rhinovirus infection in cultured bronchial epithelial cells. PMID: 11467997

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 组织特异性：
    Activated monocytes and activated T lymphocytes.
  • 数据库链接：

    HGNC: 10623

    OMIM: 602495

    KEGG: hsa:6369

    STRING: 9606.ENSP00000222902

    UniGene: Hs.247838