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  4. Human Enteropeptidase(PRSS7) ELISA kit

Human Enteropeptidase(PRSS7) ELISA kit

  • 中文名称：
    人肠肽酶(PRSS7)酶联免疫试剂盒
  • 货号：
    CSB-EL018824HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PRSS7 ELISA Kit was designed for the quantitative measurement of Human PRSS7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    TMPRSS15; ENTK; PRSS7; Enteropeptidase; Enterokinase; Serine protease 7; Transmembrane protease serine 15
  • 缩写：
    PRSS7
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %100
    Range %95-110
    1:2Average %90
    Range %85-85
    1:4Average %92
    Range %88-98
    1:8Average %98
    Range %93-104
  • 回收率：
    The recovery of human PRSS7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8984-93
    EDTA plasma (n=4)9388-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1OD2AverageCorrected
    202.528 2.422 2.475 2.358
    101.746 1.692 1.719 1.602
    51.164 1.110 1.137 1.020
    2.50.654 0.649 0.652 0.535
    1.250.413 0.434 0.424 0.307
    0.6250.271 0.253 0.262 0.145
    0.3120.195 0.208 0.202 0.085
    00.120 0.114 0.117
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
  • 基因功能参考文献：
    1. The Y174R variant showed improved specificities for substrates containing the sequences DDDDK (kcat/KM = 6.83 x 106 M-1 sec-1) and DDDDR (kcat/KM = 1.89 x 107 M-1 sec-1) relative to all other enteropeptidase variants reported to date. PMID: 23436726
    2. Enteropeptidase is a gene associated with a starvation human phenotype and a novel target for obesity treatment PMID: 23185382
    3. Human enteropeptidase shows 10x faster kinetics compared to other animal sources but low solubility under low salt conditions. A supercharged variant of enteropeptidase light chain with increased solubility was used for crystallization. PMID: 22488687
    4. Characterization of the different catalytic activity of human and bovine enteropeptidase light chains toward hydrolysis of peptides and proteins lacking tetraaspartate sequence. PMID: 22571433
    5. Engineered recombinant enteropeptidase catalytic subunit: effect of N-terminal modification PMID: 11913964
    6. Produced in enterocytes and goblet cells. Localization on brush border of cells for physiological activation of digestive enzymes. In duodenal polyps and adenocarcinoma at duodenum but not in Brunner's gland adenoma. PMID: 12907431
    7. Enterokinase directly cleaved proMMP-9 at the Lys65-Ser66 site. PMID: 18062964

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  • 相关疾病：
    Enterokinase deficiency (ENTKD)
  • 亚细胞定位：
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Peptidase S1 family
  • 组织特异性：
    Intestinal brush border.
  • 数据库链接：

    HGNC: 9490

    OMIM: 226200

    KEGG: hsa:5651

    STRING: 9606.ENSP00000284885

    UniGene: Hs.149473