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  4. Human Elastin,ELN ELISA Kit

Human Elastin,ELN ELISA Kit

  • 中文名称：
    人弹性蛋白(ELN)酶联免疫试剂盒
  • 货号：
    CSB-E09338h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ELN ELISA Kit was designed for the quantitative measurement of Human ELN protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates, urine. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

  • 别名：
    Elastin ELISA Kit; ELN ELISA Kit; ELN_HUMAN ELISA Kit; SVAS ELISA Kit; Tropoelastin ELISA Kit; WBS ELISA Kit; WS ELISA Kit
  • 缩写：
    ELN
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates, urine
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ELN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %84
    Range %80-100
    1:200Average %97
    Range %91-105
    1:400Average %97
    Range %92-112
    1:800Average %93
    Range %86-98
  • 回收率：
    The recovery of human ELN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-100
    EDTA plasma (n=4)9790-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.702 2.934 2.818 2.717
    1002.196 2.184 2.190 2.089
    501.497 1.462 1.480 1.379
    250.885 0.876 0.881 0.780
    12.50.477 0.486 0.482 0.381
    6.250.244 0.256 0.250 0.149
    3.120.152 0.161 0.157 0.056
    00.101 0.100 0.101 　
  • 本试剂盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ELN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled ELN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

平均分:
5.0分 - 1 个评价

样品类型：细胞培养上清液

样品信息：人

稀释比：没有稀释

产品评价： CSB-E09338h检测细胞培养上清弹性蛋白含量，标曲趋势准确，数据完好

By 张老师

问答及客户评论

■ 客户评论
  • Sample type: Biological fluids (Urine)
    Sample species: Human
    Review: I used this Kit to determine the elastin concentrations on Human urine samples with no dilution in duplicate and obtained %CV values of 0.2-24.4%. All my values were in the detection range, however they were in the lower end of the range. Regarding group differences it is too soon to tell, however I will be purchasing 10 more kits that will allow me to analyse these differences.
  • Sample type: Cell culture supernatant
    Sample species: Human
    Review: The error was not felt between wells of plate, and it was a reliable kit. The box was easy to use and I liked it.

靶点详情

  • 功能：
    Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
  • 基因功能参考文献：
    1. The functional role of PAX5-ELN as a potent oncoprotein in B-cell acute lymphoblastic leukemia development. PMID: 30257940
    2. Elastin plays an important role in the growth of A. fumigatus and that it participates in the formation of thick biofilm. PMID: 29452851
    3. Molecular model of human tropoelastin and implications of associated mutations PMID: 29946030
    4. Study implies that the rs2856728 variant in elastin gene polymorphisms might play crucial roles in the development and pathogenesis of intracranial aneurysms in Korean population. PMID: 29129841
    5. Degradation of tropoelastin and skin elastin by neprilysin PMID: 29196110
    6. exogenous growth factors enhance the expression of cola1, cola3, and Elastin, which is probably regulated via activating MAPK signaling pathway. PMID: 29185160
    7. In conclusion, our results support the view that lysyl oxidase (LOX) and tropoelastin are present on the cell surface and suggests the possibility that lysine oxidation by LOX precedes tropoelastin deposition onto microfibrils. PMID: 28966236
    8. Elastin degradation was correlated with age in COPD patients, smoker controls, and non-smoker controls. The correlation was weaker in the smoker control group compared with the never-smoker control or COPD group. PMID: 27587547
    9. The aim was to examine if the serum concentrations of elastin-related proteins correlate to signs of cardiovascular diseases in patients with Diabetes mellitus type 2. PMID: 28715234
    10. There is evidence that the ELN variant INT20 1315T > C is implicated in the development of intracranial aneurysm. PMID: 27687579
    11. Direct gene sequencing of ELN confirmed the diagnosis showing a previously undescribed c.2156del (p.Gly719Glufs*36) mutation in exon 30 of ELN gene. This mutation results in a shift of the reading frame. PMID: 28383366
    12. Here we report a second adult Williams-Beuren syndrome (WBS)patient with emphysema where the diagnosis of WBS was established subsequent to the discovery of severe bullous emphysema. Haploinsufficiency of ELN likely contributed to this pulmonary manifestation of WBS. PMID: 28574231
    13. the study contributes to a better understanding of the correlation between genotypic and elastin-related phenotypic features of Williams-Beuren syndrome patients PMID: 27311421
    14. We herein report the case of a Japanese female patient presenting with multiple arteriopathy including moyamoya disease, a tortuosity of abdominal arteries and pulmonary hypertension due to peripheral pulmonary artery stenosis. This case suggests the possible progression of cerebral arteriopathy including moyamoya disease in patients with elastin mutations PMID: 27080061
    15. These results indicate that elastin neoepitopes generated by the same proteases but at different amino acid sites provide different tissue-related information depending on the disease in question. PMID: 23805173
    16. Tropoelastin interacts with cells through cell surface receptors including integrins and glycosaminoglycans (GAGs); study mapped a cell-interactive sequence of tropoelastin to domain 17 and the first six amino acids of domain 18. PMID: 28544621
    17. Tropoelastin acts through a PI3K-specific pathway that leads to the phosphorylation of eNOS to enhance nitric oxide production in endothelial cells. PMID: 27175893
    18. Deficient circumferential growth is the predominant mechanism for moderate obstructive aortic disease resulting from partial elastin deficiency in Williams syndrome. PMID: 28254817
    19. In this report we describe a three-generation family suffering from supravalvular aortic stenosis, various other arterial stenoses, sudden death, and intracranial aneurysms. A frameshift mutation in exon 12 of the elastin gene, not described before, was detected in the affected family members. PMID: 27866049
    20. Data show that skin aging is associated with the decomposition of elastin fibers, which is more pronounced in sun-exposed tissue. PMID: 27569260
    21. Transcriptional and posttranscriptional mechanisms contribute to the dysregulation of elastin expression and elastogenesis in Schimke immuno-osseous dysplasia. PMID: 26309238
    22. Neutrophil elastase degraded elastin fragments are increased in the serum of patients with idiopathic pulmonary fibrosis and lung cancer compared to controls. PMID: 25935650
    23. Data suggest that MFAP4 (microfibrillar-associated protein 4) binds tropoelastin, fibrillin-1/2, and elastin cross-linking amino acid desmosine; MFAP4 co-localizes with fibrillin-1-positive fibers; MFAP4 actively promotes tropoelastin self-assembly. PMID: 26601954
    24. In asthma, MMP-1 and MMP-2 mediate IL-13-induced suppression of ELN expression in airway fibroblasts. PMID: 26074138
    25. Current knowledge about elastin peptides relevant to cardiovascular pathologies to further delineate their potential application in cardiovascular disease. [Review] PMID: 25794852
    26. elastin may be important for plaque stability PMID: 25803692
    27. Data suggest that cross-linking involving desmosine and isodesmosine residues in human tropoelastin and bovine elastin contributes to long-term stability of these proteins. PMID: 25604393
    28. Studies indicate that the low sequence complexity, phase behavior, and elastic properties make elastin-like polypeptides (ELPs) an interesting intrinsically disordered proteins (IDP). PMID: 26325592
    29. Supravalvular aortic aneurysms caused by a familial 7q11.23 triplication - including the ELN gene. PMID: 24932728
    30. Up-regulation of elastin and fibulin-5 mRNA levels in ICA were strongly correlated with family history of cardiovascular disease when compared to CCA PMID: 25792650
    31. there may be significantly different associations in genetic variants of elastin between two angiographic phenotypes of polypoidal choroidal vasculopathy (the significant association of rs868005 with Type 2, but not with Type 1) PMID: 25775011
    32. Pathogenetic stimuli induced a significant increase in the expression of LOXL1 and elastic proteins and resulted in their assembly into exfoliation syndrome-like fibrils in vitro. PMID: 25275906
    33. Collectively, these results suggest that dysregulation of LOXL1 expression is a contributing factor to exfoliation disease development. PMID: 25275910
    34. the role of the Asp-72 site in stabilizing the N-terminal segment of human tropoelastin PMID: 25342751
    35. Overall the results indicate that LTBP-2 may have a negative regulatory role during elastic fiber assembly, perhaps in displacing elastin microassemblies from complexes with fibulin-5 and/or cell surface heparan sulfate proteoglycans. PMID: 24148803
    36. The decrease in age-adjusted circulating elastin levels is a potential indicator of eventual cardiovascular dysfunction in medical check-up individuals, as predicted by the risk factors dyslipidemia, hyperuricemia or diabetes. PMID: 23756406
    37. Mutational analysis of the elastin gene identified heterozygosity for a previously unreported de novo c.2184delT mutation in exon 30 not present in either parent. PMID: 24758204
    38. Several extracellular matrix constituents, LOXs, FBN1, and FBLN5, implicated in the development of elastin, are overexpressed in the subepithelial connective tissue extracellular matrix of human pterygium PMID: 25183766
    39. AAA-SMCs have a unique gene expression profile and a proelastolytic phenotype that is augmented by macrophages. PMID: 24080131
    40. Discovery of retinal elastin and its possible role in age-related macular degeneration. PMID: 24232693
    41. Construction and application of elastin like polypeptide containing IL-4 receptor targeting peptide. PMID: 24339977
    42. Tropoelastin modulates TGF-beta1-induced expression of VEGF and CTGF in airway smooth muscle cells. PMID: 23597635
    43. study indicates that the elastin gene may be associated with the formation of IAs, and importantly, that it may also be associated with the rupture of IAs. PMID: 23294012
    44. the sequence of cross-linking domains has a dramatic effect on self-assembly properties of elastin-like polypeptides, and the presence of lysine residues in these domains may serve to prevent inappropriate ordered aggregation. PMID: 24550393
    45. These data reveal a common alphaV integrin-binding theme for tropoelastin: alphaVbeta3 at the C terminus and alphaVbeta5 at the central region of tropoelastin. PMID: 24293364
    46. The s believe that the Williams-Beuren syndrome chromosome region can be a possible target for further investigation as the genetic basis of keratoconus. PMID: 23167938
    47. Evaluation of these chimeras revealed two exosites contributing to the elastolytic activity of cathepsin V that are distant from the active cleft of the protease and are located in surface loop regions. PMID: 24121514
    48. Elastin binding protein and FKBP65 modulate the kinetics of self-assembly of tropoelastin in an in vitro system. PMID: 24106871
    49. Connective tissue growth factor leads to maximal elastin increase in human umbilical cord-derived mesenchymal stem cells after 7 days of chondroinduction and not in undifferentiated mesenchymal stem cells. PMID: 23542855
    50. Single nucleotide polymorphisms in the IGF2, CCL2, and ELN genes may be associated to the degree and recovery time of non-contact soft tissue injuries. PMID: 23890452

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  • 相关疾病：
    Cutis laxa, autosomal dominant, 1 (ADCL1); Supravalvular aortic stenosis (SVAS)
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Elastin family
  • 组织特异性：
    Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
  • 数据库链接：

    HGNC: 3327

    OMIM: 123700

    KEGG: hsa:2006

    STRING: 9606.ENSP00000252034

    UniGene: Hs.647061