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  4. Human EGF-like repeat and discoidin I-like domain-containing protein 3(EDIL3) ELISA kit

Human EGF-like repeat and discoidin I-like domain-containing protein 3(EDIL3) ELISA kit

  • 中文名称：
    人EGF样重复序列和盘状结构域蛋白3(EDIL3)酶联免疫试剂盒
  • 货号：
    CSB-EL007399HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The product CSB-EL007399HU is a sandwich ELISA kit developed to measure concentrations of human EDIL3 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of EDIL3 in samples. The color intensity is measured at 450 nm via a microplate reader.

    As a pro-angiogenic factor, EDIL3 mediates the immune and anti-inflammatory response. EDIL3 acts as a regulator of endothelial cell adhesion and migration. Additionally, Edil3 has been described as an autocrine or paracrine secreted protein that participates in endothelial cell migration, angiogenesis, and branching morphogenesis of blood vessels. It has been reported that EDIL3 is overexpressed in several tumor types, including bladder, pancreas, breast, and liver carcinomas. Overexpression of EDIL3 promotes carcinogenesis by reducing apoptosis in cancer cells and stimulating cancer vascularization. It is associated with tumor progression and poor prognosis.

  • 别名：
    DEL1 ELISA Kit; Developmental endothelial locus 1 ELISA Kit; Developmentally regulated endothelial cell locus 1 protein ELISA Kit; Developmentally-regulated endothelial cell locus 1 protein ELISA Kit; Edil3 ELISA Kit; EDIL3_HUMAN ELISA Kit; EGF like repeat and discoidin I like domain containing protein 3 ELISA Kit; EGF like repeats and discoidin I like domains 3 ELISA Kit; EGF like repeats and discoidin I like domains protein 3 ELISA Kit; EGF-like repeat and discoidin I-like domain-containing protein 3 ELISA Kit; Integrin binding protein DEL1 ELISA Kit; Integrin-binding protein DEL1 ELISA Kit
  • 缩写：
    EDIL3
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human EDIL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %80-95
    1:2Average %95
    Range %89-100
    1:4Average %99
    Range %91-110
    1:8Average %94
    Range %88-98
  • 回收率：
    The recovery of human EDIL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9280-98
    EDTA plasma (n=4)9692-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.033 1.872 1.953 1.765
    40001.598 1.476 1.537 1.349
    20001.077 1.014 1.046 0.858
    10000.774 0.729 0.752 0.564
    5000.513 0.494 0.504 0.316
    2500.374 0.359 0.367 0.179
    1250.291 0.278 0.285 0.097
    00.190 0.186 0.188 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.
  • 基因功能参考文献：
    1. EDIL3 may be implicated in retinal neovascularization in vitro. Silencing EDIL3 expression was demonstrated to impair the proliferative, migratory and tube forming capabilities of HRECs in vitro. PMID: 28765888
    2. these data reveal an unrecognised function of endogenous Del-1 as a local inhibitor of ischaemia-induced angiogenesis by restraining LFA-1-dependent homing of pro-angiogenic haematopoietic cells to ischaemic tissues PMID: 28447099
    3. Del-1-induced HSC proliferation and myeloid lineage commitment were mediated by beta3 integrin on hematopoietic progenitors. PMID: 28846069
    4. Del-1 pre-treatment of blood before addition of islets diminished coagulation activation and islet damage PMID: 26676803
    5. Del-1 on circulating EVs is a promising marker to improve identification of patients with early-stage breast cancer and distinguish breast cancer from benign breast tumors and noncancerous diseases. PMID: 26603257
    6. Overexpressed EDIL3 promotes anchorage-independent tumor growth in human pancreatic cancer. PMID: 26735172
    7. Overexpression of the Del-1 gene potentiates proliferation and invasion of lung carcinoma cells. PMID: 26545781
    8. The importance of interaction between EDIL3 and integrin alphaV. PMID: 25273699
    9. The reciprocal regulation between Del-1 and inflammation may contribute to optimally balance the protective and the potentially harmful effects of inflammatory cell recruitment. PMID: 24416060
    10. Downregulation of developmentally regulated endothelial cell locus-1 inhibits the growth of colon cancer. PMID: 19292890
    11. Del-1 may act as a gatekeeper of adrenal gland inflammation and may regulate the integrity of the hypothalamic-pituitary-adrenal axis stress response, thereby modulating adrenal (dys)function in the course of SIRS. PMID: 23364949
    12. database search for EGF domain sequences shows that this RGD finger is likely an evolutionary insertion and unique to the EGF domain of Del-1 PMID: 22601780
    13. Del-1 mediates phosphatidylserine- and integrin-dependent endothelial uptake of microparticles by endocytosis. PMID: 22388320
    14. High expression level of EDIL3 predicts poor prognosis of hepatocellular carcinoma patients. PMID: 20857535
    15. Del1 mediates vascular smooth muscle cell adhesion, migration, and proliferation through interaction with integrin alpha(v)beta(3). PMID: 11959660
    16. acts as an angiogenic factor in the context of solid tumor formation; the increase in vascularization accelerates tumor growth through decreased apoptosis PMID: 12074641
    17. Alpha v beta 5, alpha v beta 5 and their ligands Del-1 and L1 play an important role in the process of tumor cells moving from the original place. PMID: 18090124

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  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    HGNC: 3173

    OMIM: 606018

    KEGG: hsa:10085

    STRING: 9606.ENSP00000296591

    UniGene: Hs.482730