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  4. Human Desert hedgehog (DHH) ELISA kit

Human Desert hedgehog (DHH) ELISA kit

  • 中文名称：
    人沙漠刺猬因子(DHH)酶联免疫试剂盒
  • 货号：
    CSB-E12006h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human DHH ELISA Kit was designed for the quantitative measurement of Human DHH protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

  • 别名：
    C78960 ELISA Kit; Desert hedgehog (Drosophila) homolog ELISA Kit; Desert hedgehog ELISA Kit; Desert hedgehog homolog (Drosophila) ELISA Kit; Desert hedgehog protein C-product ELISA Kit; Desert hedgehog protein precursor ELISA Kit; DHH ELISA Kit; DHH_HUMAN ELISA Kit; GDXYM ELISA Kit; HHG-3 ELISA Kit; Hira ELISA Kit; SRXY7 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Stem Cells
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %89
    Range %84-92
    1:10Average %99
    Range %95-101
    1:20Average %99
    Range %95-102
    1:40Average %95
    Range %90-98
  • 回收率：
    The recovery of human DHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9491-96
    EDTA plasma (n=4)9694-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.418 2.402 2.410 2.297
    5001.766 1.880 1.823 1.710
    2501.223 1.213 1.218 1.105
    1250.689 0.707 0.698 0.585
    62.50.453 0.432 0.443 0.330
    31.20.324 0.331 0.328 0.215
    15.60.224 0.218 0.221 0.108
    00.115 0.111 0.113 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.
  • 基因功能参考文献：
    1. Whole-exome sequencing revealed a homozygous variant in DHH leading to the p.Trp173Cys substitution. The relevant Trp residue is conserved, and its alteration was predicted to be deleterious. Molecular dynamics simulations showed that the mutation increases the conformational flexibility of the protein PMID: 28708305
    2. Single nucleotide polymorphism in the DHH gene is associated with bipolar disorder. PMID: 25517604
    3. Mutations in DHH play a role in 46,XY gonadal dysgenesis and are associated with seminoma formation and a neuropathy with minifascicle formation. PMID: 25927242
    4. Findings are unprecedented and indicate that the DHH-RHEBL1 fusion transcript is a novel recurrent feature in the changing landscape of CBFA2T3-GLIS2-positive childhood AML. PMID: 24127550
    5. Mutations in DHH are associated with 46,XY pure gonadal dysgenesis and mixed gonadal dysgenesis. PMID: 23786321
    6. This study demonistrated that Desert hedgehog links transcription factor Sox10 to peripheral nerve development. PMID: 22514309
    7. Studies indicate that pathways of Hedgehog (Hh), Wnt and Notch, which regulate development during embryonic life and somatic stem cells (SCs) in the adult organism, can be reactivated in malignancies and support tumor-initiating cells (TIC) scompartment. PMID: 22123293
    8. Two cases have been described in Indian patients with 46,XY complete gonadal dysgenesis that could be attributable to mutations in the Desert hedgehog (DHH) gene leading to non-functional DHH protein. PMID: 21816240
    9. We found no significant correlation between the expression of SOX9 and desert hedgehog, and neither SOX9 nor desert hedgehog expression was correlated to the histoprognostic grade in sarcomas. PMID: 21193222
    10. These data demonstrate that DHH is a key molecule in both male gonadal differentiation and perineurial formation in peripheral nerves PMID: 11990454
    11. Importance of DHH in mammalian male sexual differentiation, providing extended evidence that DHH constitutes a key gene in gonadal differentiation PMID: 16390857
    12. its signal transduction regulates tumor development. (review) PMID: 18788453
    13. Hh signaling is important in the pathogenesis of B-CLL and, hence, may be a potential therapeutic target PMID: 19074837

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  • 相关疾病：
    Partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD); 46,XY sex reversal 7 (SRXY7)
  • 亚细胞定位：
    [Desert hedgehog protein N-product]: Cell membrane; Lipid-anchor; Extracellular side.; [Desert hedgehog protein C-product]: Secreted, extracellular space.
  • 蛋白家族：
    Hedgehog family
  • 数据库链接：

    HGNC: 2865

    OMIM: 233420

    KEGG: hsa:50846

    STRING: 9606.ENSP00000266991

    UniGene: Hs.524382