Human Dehydroepiandrosterone sulfate,DHEA-S ELISA Kit

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产品详情

产品描述：

This Human DHEA-S ELISA Kit was designed for the quantitative measurement of Human DHEA-S protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 20 ng/mL-4000 ng/mL and the sensitivity is 10 ng/mL.
缩写：

DHEA-S
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

20 ng/mL-4000 ng/mL
灵敏度：

10 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-103
1:2	Average %	96
	Range %	89-105
1:4	Average %	94
	Range %	90-100
1:8	Average %	90
	Range %	83-110

回收率：

The recovery of human DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-103
EDTA plasma (n=4)	89	84-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.