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  4. Human Cytidine deaminase(CDA) ELISA kit

Human Cytidine deaminase(CDA) ELISA kit

  • 中文名称：
    人胞苷脱氨酶(CDA)酶联免疫试剂盒
  • 货号：
    CSB-EL004976HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CDA ELISA Kit was designed for the quantitative measurement of Human CDA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    CDA ELISA kit; CDDCytidine deaminase ELISA kit; EC 3.5.4.5 ELISA kit; Cytidine aminohydrolase ELISA kit
  • 缩写：
    CDA
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CDA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 84
    Range % 80-92
    1:2 Average % 97
    Range % 91-105
    1:4 Average % 102
    Range % 98-110
    1:8 Average % 93
    Range % 86-98
  • 回收率：
    The recovery of human CDA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-100
    EDTA plasma (n=4) 88 84-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 1.890 1.952 1.921 1.766
    500 1.338 1.316 1.327 1.172
    250 0.924 0.904 0.914 0.759
    125 0.585 0.599 0.592 0.437
    62.5 0.402 0.424 0.413 0.258
    31.25 0.321 0.309 0.315 0.160
    15.6 0.238 0.229 0.234 0.079
    0 0.153 0.157 0.155 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

i curiously of human CDA ELISA kit about the last step
the protocol say using 450 nm to read plate but also saying can use 540 or 570 nm if availability and 450 nm may be higher and less accurate?? and what kind wavelength do i use?
450nm? 540 nm ? or 570 nm
or which wavelength is you recommended?

A:
Thanks for your inquiry!
CSB-EL004476HU
We tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test human fecal samplee ,we suggest you do a pretest . Pls find following sample preparation method.
Feces Dilute samples with appropriate Sample Diluent. This can be achieved by adding 0.1g sample to appropriate Sample Diluent. Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.
  • 基因功能参考文献：
    1. SNPs in CDA and CES2 were associated with benefit from the addition of capecitabine to chemotherapy in metastatic breast cancer patients PMID: 28827188
    2. The observed incomplete sister chromatid disjunction may be due to the accumulation of unreplicated DNA during mitosis in CDA-deficient cells, as reflected in the changes in centromeric DNA structure associated with the decrease in basal PARP-1 activity. PMID: 28463527
    3. Intracerebral microdialysis revealed that clonal NSC line to stably express cytosine deaminase (CD-NSCs) produced 5-FU locally in the brain in a 5-FC dose-dependent manner. Collectively, our results from this first-in-human study demonstrate initial safety and proof of concept regarding the ability of NSCs to target brain tumors and locally produce chemotherapy. PMID: 27979915
    4. results indicate that rs2072671 in CDA may be an important prognostic marker in NK-AML patients. PMID: 26354033
    5. tumor cell lines that are susceptible to epigenetic nucleosides overexpress cytidine deaminase (CDA); CDA converts 5hmdC and 5fdC into variants of uridine that are incorporated into DNA, resulting in accumulation of DNA damage, and ultimately, cell death PMID: 26200337
    6. RNA expression of deoxycytidine kinase (DCK), human equilibrative nucleoside transporter-1 (ENT1) and ribonucleotide reductase M1 (RRM1) were significantly higher and cytidine deaminase (CDA) was significantly lower in ex vivo Ara-C sensitive samples. PMID: 26083014
    7. stimulation of PARP-1 activity in CDA-deficient cells restores replication and, thus, chromosome segregation. Moreover, increasing intracellular dCTP levels generates under-replication-induced sister-chromatid bridges as efficiently as PARP-1 knockdown PMID: 26181065
    8. n the multivariate Cox regression analysis, we found that age at diagnosis, wild-type genotype of the CDA A79C polymorphism, and wild-type genotype of the dCK C360G polymorphism were the most significant prognostic factors for predicting the risk of death PMID: 26090398
    9. Single nucleotide polymorphisms in cytidine deaminase gene were associated with the efficacy of gemcitabine in Biliary Tract Cancer. PMID: 26418006
    10. Polymorphisms in ABCB1, CDA, ENOSF1,and TYMS could help to predict specific and overall severe adverse reactions to capecitabine. PMID: 25691056
    11. miR-484-modulated cytidine deaminase has a dual impact in promoting chemoresistance and suppressing cell proliferation in breast cancer PMID: 25643696
    12. This case report calls for further prospective studies investigating the exact role that CDA status plays in the clinical outcome of patients treated with azacytidine. PMID: 25850965
    13. The A79C CDA polymorphism did not show a significant impact on the response rate to gemcitabine in NSCLC patients, while the wild type CDA genotype was indeed correlated to a lower rate of incidence of severe anemia in patients taking gemcitabine. PMID: 24557790
    14. These results suggest that CDA 79A>C polymorphisms is a potential biomarker for toxicity of gemcitabine-based chemotherapy and a CDA testing before gemcitabine administration is preferred. PMID: 25582275
    15. These results provide suggestive evidence of a favorable effect for the XPD 312Asp/Asp and XPD 751Lys/Lys genotypes with respect to overall survival rates in platinum-treated NSCLC patients PMID: 24841663
    16. Macrophages mediate gemcitabine resistance of pancreatic adenocarcinoma by upregulating cytidine deaminase. PMID: 23995783
    17. The T70 variant has a lower catalytic efficiency toward the analyzed substrates when compared to the A70 variant, suggesting that patients carrying the 208G>A SNP may have a greater exposure to cytosine based pro drugs PMID: 24183806
    18. Carriers of the CDA*2B haplotype displayed higher CDA activity. PMID: 23651026
    19. ultrarapid metabolizer patients are nearly five-times more likely to have progressive disease than patients with normal or low CDA activities. PMID: 23837479
    20. Studied human CDA using site-directed mutagenesis: through these studies it was possible to understand the role exerted by specific amino acid residues in CDA active site and in the contacts between subunits. PMID: 23033855
    21. Variation in the DPYD, TYMS, CDA and MTHFR genes are clinically significant predictors of fluoropyrimidine toxicity. PMID: 23736036
    22. Data indicate cytidine deaminase rapid expression upon doxycycline application in mouse haematopoietic system. PMID: 22592598
    23. In conclusion, in this prospective randomized adjuvant study of patients with pancreatic cancer, the cytidine deaminase polymorphism is validated as a predictive marker of gemcitabine hematological toxicity, but not with treatment response or survival PMID: 21625252
    24. It is evident that the DCK and CDA polymorphisms might be the important markers for the AML patients' therapy outcomes in a Chinese population. PMID: 22884143
    25. Chinese patients carrying A79C variant C allele of CDA were found to be at higher risk of developing severe neutropenia after gemcitabine-based chemotherapy. PMID: 22546611
    26. CDA RNA expression as well as Ara-C IC showed wide variation in AML samples and normal controls. PMID: 22304580
    27. cytidine deaminase enzymatic activity appears to be the strongest candidate biomarker of activity and efficacy of platinum-gemcitabine-based chemotherapy in advanced non-small-cell lung cancer patients PMID: 21652582
    28. Cytidine deaminase (CDA) 435 T/T genotype was significantly associated with better response to treatment and the CDA 435 C/T genotype was associated with a significantly increased risk of nonhematological toxicity. PMID: 22052224
    29. Data show that the CDA/DCK ratio was 3 fold higher in non-responders than responders (P<.05), suggesting that this could be a mechanism of primary resistance. PMID: 21858090
    30. Aberrant activation-induced cytidine deaminase expression is correlated with persistent inflammatory condition induced by H. pylori infection and may contribute to the development of gastric cancer through an inflammatory condition and intestinalization. PMID: 21538122
    31. the deleted allele of rs3215400 in the promoter of cytidine deaminase shows an increased allele-specific expression and is significantly associated with an increased risk of capecitabine-induced hand-foot syndrome PMID: 21325291
    32. Single nucleotide polymorphisms in cytidine deaminase is associated with the pharmacological advantage of prolonged dose rate gemcitabine. PMID: 21332653
    33. Compared with their parental cells, the expressions of CDA, RRM1, PTEN and ERCC1 increase in human gemcitabine-resistant non-small cell lung cancer cell lines. PMID: 20211060
    34. These investigations reinforced the hypothesis that in human CDA the side chain of Y33 is involved in intersubunit interactions with four glutamate residues forming a double latch that connects each of the two pairs of monomers of the tetrameric CDA. PMID: 20637228
    35. The aim of this study was also to assess the distribution of genotypic variants of CDA in a central Italy population. PMID: 19941076
    36. The cytidine deaminase 208A genotype is shown to be more sensitive to cytosine arabinoside than the 208G genotype in childhood acute leukemia. PMID: 19035178
    37. Activation-induced cytidine deaminase expression suggests diffuse large B-cell lymphoma may arise from a putative interfollicular large B cell. PMID: 20196672
    38. Curcumin downregulated Helicobacter pylori induced cytidine deaminase expression in gastric epithelial cells. PMID: 19889077
    39. serum adenosine deaminase and cytidine deaminase levels were significantly higher in systemic lupus erythematosus patients PMID: 12113294
    40. This enzyme can act on deoxycytidine in single-stranded DNA, but requires the action of RNase A. PMID: 12651944
    41. The level of CDD mRNA expression varies at the different stage of acute leukemia. The expression level of CDD seems not to be a prognostic factor. PMID: 12844405
    42. Intersubunit interactions in human cytidine deaminase using wild and recombinant, amino acid-substituted enzymes PMID: 14565460
    43. Understanding the catalytic mechanism by crystallization of the E. coli homolog PMID: 14565461
    44. Presence of several GATA1 binding sites in the CDAsf promoter and the uniform detection of GATA1 mutations in DS megakaryocytic leukemia suggested the potential role of GATA1 in regulating CDA transcription. PMID: 14744791
    45. analysis of isoenzymatic forms of human cytidine deaminase PMID: 15713780
    46. Molecular model of cytidine deaminase. PMID: 16303324
    47. Gene transfer increased the resistance of CDD-transduced cord blood and peripheral blood-derived progenitor cells for 20-100 nM cytarabine and 8-10 nM gemcitabine. Protection was observed for progenitors of erythroid as well as myeloid differentiation. PMID: 16304576
    48. 6 SNPs ( -92A>G, -205C>G, -451C>T, -897C>A, -1075A>G and -1181G>A) in promoter of CDA may influence Ara-C chemosensitivity. PMID: 16446974
    49. Described the identification and characterization of HSCD, which is the product of alternative splicing of the HCD gene PMID: 17464349
    50. After gene trasfer of CDA, this enzyme plus 5-fluorocytosine with TRAIL may be useful for the therapy of tumors resistant to TRAIL. PMID: 17479107

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  • 蛋白家族：
    Cytidine and deoxycytidylate deaminase family
  • 组织特异性：
    Highly expressed in granulocytes while expression is very low in fibroblasts, chondrocytes, monocytes, and T- as well as B-cell lines.
  • 数据库链接：

    HGNC: 1712

    OMIM: 123920

    KEGG: hsa:978

    STRING: 9606.ENSP00000364212

    UniGene: Hs.466910