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  4. Human Cystatin C,Cys-C ELISA Kit

Human Cystatin C,Cys-C ELISA Kit

  • 中文名称：
    人胱抑素C(Cys-C)酶联免疫试剂盒
  • 货号：
    CSB-E08384h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        CST3是一种蛋白酶抑制剂，也被称为卵白素酶抑制剂（SERPINA3），广泛存在于多种组织和体液中，包括血清、脑脊液、尿液等。CST3参与多种生物学过程，如细胞凋亡、细胞增殖、细胞迁移和炎症反应等。CST3通过抑制蛋白酶的活性，调节细胞内外的蛋白酶活性平衡，从而维持正常的生理状态。此外，CST3还与多种疾病的发生和发展密切相关，如阿尔茨海默病、癌症、糖尿病等。
        j9九游会登录入口首页生物所提供的Human Cystatin C,Cys-C ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、尿液、唾液、组织匀浆样本中的CST3，其灵敏度为5.824 ng/mL，检测范围为7.8 ng/mL-500 ng/mL。
     
  • 别名：
    AD 8 ELISA Kit; AD8 ELISA Kit; Amyloid angiopathy and cerebral hemorrhage ELISA Kit; ARMD11 ELISA Kit; bA218C14.4 (cystatin C) ELISA Kit; bA218C14.4 ELISA Kit; Cst 3 ELISA Kit; Cst3 ELISA Kit; CST3 protein ELISA Kit; Cystatin 3 ELISA Kit; Cystatin-3 ELISA Kit; Cystatin-C ELISA Kit; Cystatin3 ELISA Kit; CystatinC ELISA Kit; CYTC_HUMAN ELISA Kit; Epididymis secretory protein Li 2 ELISA Kit; Gamma trace ELISA Kit; Gamma-trace ELISA Kit; HCCAA ELISA Kit; HEL S 2 ELISA Kit; MGC117328 ELISA Kit; Neuroendocrine basic polypeptide ELISA Kit; Post gamma globulin ELISA Kit; Post-gamma-globulin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, urine, saliva, tissue homogenates
  • 检测范围：
    7.8 ng/mL-500 ng/mL
  • 灵敏度：
    5.824 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

     

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human CYSC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

  • 标准曲线：

     

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

我可以用该试剂盒（CSB-E08384h）检测浸泡组织中的胱抑素C吗？

A:
是浸泡吗 用什么溶液浸泡的 是甲醛？不过我们确实没处理过类似的样本，而且对于tissue样本的均一性差异较大，因此不论什么处理方法，梯度预实验是必不可少的；而对于CSB-E08384h指标则要尽可能避免影响蛋白结构的试剂（比如甲醛）。

靶点详情

  • 最新研究进展：
    CST3是一种抑制酶类蛋白质，也称为肝素化相关载体蛋白（HRP），它在细胞生长、增殖和分化中起着重要的作用。最新研究表明，CST3参与调节免疫系统和肿瘤发展，对多种疾病的发生和发展具有重要的生物学功能和临床价值，如肿瘤、神经系统疾病、心脑血管疾病等。研究者正在通过研究CST3的生物学功能和临床应用来进一步深入了解这个蛋白质的作用机制和应用价值。
     
  • 功能：
    As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • 基因功能参考文献：
    1. Urinary cystatin-C levels were increased in type 2 diabetic patients with early diabetic nephropathy. PMID: 29673928
    2. Age and cystatin C were found to be significant risk factors for cardiovascular events in a pre-diabetic cohort. PMID: 29661604
    3. Increased cystatin C levels may be associated with enhanced risks of composite endpoints in patients with ST-elevation acute myocardial infarction undergoing elective percutaneous coronary intervention PMID: 29891036
    4. Data suggest that kinetics of degradation/proteolysis versus of aggregation of amyloid beta(1-40) and amyloid beta(1-42) at specific concentrations of amyloid beta, cathepsin B, and cystatin C can be modeled and predicted. PMID: 29046353
    5. Serum CysC, at the time of CRRT (Continuous Renal Replacement Therapy cessation), is an independent predictor of the successful weaning from CRRT in critically ill patients with acute kidney injury. PMID: 29870981
    6. The serum levels of cystatin C and urine NGAL, urine OPN can be used as a good marker for assessing the renal effect of obesity which can lead end stage renal disease in pediatric population. PMID: 30035656
    7. The average of estimated glomerular filtration rate (eGFRave) based on creatinine (eGFRcre) and eGFR based on cystatin C (eGFRcys) best predicted the exacerbation of renal function in allogeneic hematopoietic cell transplantation (allo-HCT). PMID: 29086660
    8. Studied serum Cystatin C as a biomarker in bladder urothelial neoplasm; results found serum Cystatin C not to be a reliable marker in predicting bladder urothelial tumors tumorigenesis and clinicopathological characteristics. PMID: 29789781
    9. bladder cancer (BCa) patients have lower serum cysC concentration than the control group. Serum cysC may be considered as a potential marker of BCa but not its aggressiveness. PMID: 29749360
    10. Postoperative serum Cystatin C, IL-18 and Uric acid after 24h were significantly associated with acute kidney injury PMID: 29627485
    11. Serum CysC is a strong predictor of AKI and the short-term prognosis of CCU patients. PMID: 29179178
    12. Neutrophil gelatinase-associated lipocalin concentrations in the urine and serum on the second day of intensive care unit admission could be used to predict the development of acute kidney injury in the following three to seven days in the intensive care unit; however, the cystatin C concentration did not have predictive value PMID: 28142264
    13. Study demonstrated that the binding of CST3 and PSAP decreased the inhibitory effects of CST3 on proteinase in vitro. The co-localization of both proteins was detected in cultured cells and in Bunina body-containing motor neurons from patients with amyotrophic lateral sclerosis, suggesting that they might be involved in the process of Bunina body formation. PMID: 29249381
    14. elevated cystatin C is independently associated with adverse vascular outcomes in people with suspected or established CAD in terms of all-cause mortality, cardiovascular mortality, and total adverse vascular events [meta-analysis] PMID: 28599259
    15. Cognitive function was significantly and inversely associated with cystatin C and microalbuminuria, in the relatively younger general Japanese population. PMID: 28865325
    16. This paper discusses the current findings regarding the role of Cyst C in cancer progression and the clinical implications emerging from these studies--{review} PMID: 29654808
    17. We enrolled 43 stable coronary artery disease (CAD) male patients to examine the relationship between cystatin C-adiponectin (CysC-APN) complex and coronary plaque characteristics. In multivariate analysis, CysC-APN complex levels were identified as the strongest negative factor for fibrotic components and the strongest positive factor for both necrotic and lipidic plus necrotic components. PMID: 28321013
    18. Urinary cystatin C is an independent risk predictor in patients with acute myocardial infarction. PMID: 28778467
    19. serum Cys-C levels predict renal function in patients with prostate neoplasia, but were not a biomarker for the development of prostate neoplasia, and were not correlated with the clinicopathological characteristics of prostate cancer . PMID: 28812020
    20. There were no associations between serum cysC levels and renal involvement in patients with primary Sjogren's syndrome. PMID: 25367815
    21. cystatin-C should be considered an early biomarker of AKI PMID: 28552392
    22. CysC is as an early acute kidney injury biomarker and predictive of persistent acute kidney injury on aminoglycoside treatment. PMID: 27743042
    23. Cystatin C may be considered a biological marker for vascular dysfunction and subclinical atherosclerosis in SCD. PMID: 27582023
    24. Serum cystatin C level was significantly associated with subclinical atherosclerosis. An increase in cystatin C level could be a valuable surrogate marker for the risk of cardiovascular disease in patients with type 2 diabetes mellitus. PMID: 29090609
    25. Mild-to-moderate kidney dysfunction modifies the relationship between CysC and high-risk coronary lesion, but not NGAL. PMID: 27680774
    26. Increasing cystatin C and cathepsin B reported in serum of colorectal cancer patients. PMID: 28182342
    27. Both cystatin C and beta 2 macroglobulin are elevated in type 2 diabetics with diabetic nephropathy. Cystatin C may be useful in the early diagnosis of diabetic nephropathy PMID: 28182344
    28. Results from nuclear magnetic resonance structural data and molecular dynamics modeling point to conformational requirements and special importance of some amino acid residues in cystatin 3 C-terminal for the its interaction with amyloid beta fragments. PMID: 27714883
    29. The findings of the current studies may be important for future design of hCC dimerization inhibitors. PMID: 27573935
    30. High cystatin C was associated with increased risk of mortality but not with poor functional outcome in patients with acute intracerebral hemorrhage. PMID: 27578255
    31. this results identified cystatin 3 as a biomarker in patients with seasonal allergic rhinitis PMID: 28633877
    32. Serum and urine levels of cystatin C were significantly higher in children with acute kidney injury. [Review and Meta-Analysis] PMID: 28372557
    33. serum level in acute kidney injury patients helps predict risk of chronic kidney disease stage 3 PMID: 27658322
    34. Circulating CysC and Gal-3 were associated with greater left atrial dilatation in patients with heart failure with reduced left ventricular ejection fraction. PMID: 28830298
    35. Application of the cystatin C-based equations most accurately estimate glomerular filtration rate in Chinese patients with chronic kidney disease. PMID: 27125433
    36. This study tested the utility of neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C (CysC) in addition to standard creatinine for early determination of Acute Kidney Injury (AKI) After Heart Operations Using Cardiopulmonary Bypass. The results support the notion that CysC and NGAL should be viewed as comparable for early AKI detection. PMID: 27862029
    37. The strong association between epicardial adipose tissue (EAT) and cystatin C demonstrated herein indicates that EAT accumulation may play an important role in Cystatin C secretion, possibly contributing to cardiometabolic risk in T2DM patients. PMID: 28922364
    38. A high CysC level had predictive value regarding the severity of vascular lesions in acute coronary syndrome. PMID: 28143410
    39. Elevated levels of cystatin C were detected in early diagnosis of right heart failure in patients with systemic sclerosis. PMID: 27414726
    40. biomarker of renal dysfunction, acute-on-chronic liver failure, and hepatorenal syndrome in decompensated liver cirrhosis PMID: 28545169
    41. Data suggest that melanoma cells internalize/absorb cystatin C from culture media, leading to increased intracellular cystatin C levels; cystatin E/M is internalized as well but at modest rate due to down-regulation of cell migration; however, the effect of intracellular cystatin E/M on down-regulation of legumain activity is pronounced. PMID: 28630039
    42. An elevated preoperative Cys-C level was demonstrated to be related with worse survival in patients with renal cell carcinoma(RCC). Measuring preoperative serum Cys-C might be a simple way for finding poor prognostic patients and patients with elevated preoperative Cys-C level should be more closely followed up PMID: 28586363
    43. Acute kidney injury is common following deliberate self-poisoning with a combination washing powder containing oxalic acid and potassium permanganate and cystatin C monitoring may under estimate the severity of poisoning. PMID: 28535124
    44. Cystatin C significantly decreased the lipopolysaccharide-stimulated release and expression of interleukin-1beta and tumor necrosis factor-alpha in monocyte and peripheral blood mononuclear cultures from healthy donors and Crohn's patients. Cystatin C was internalized by monocytes via an active endocytic process, decreased phosphorylation of the MAP kinase pathway ERK-1/2, and altered surface markers. PMID: 27190304
    45. Fibrillation of CysC therefore likely initiates from the monomer and does not require domain-swapping. The non-swapped oligomers likely represent a dead-end offshoot of the amyloid pathway and must dissociate to monomers prior to rearranging to amyloid fibrils. These prefibrillar CysC oligomers were potent inhibitors of aggregation of the Alzheimer's-related peptide, beta-amyloid. PMID: 28487367
    46. CysC plays an important regulatory role in combating cell death via the autophagic pathway in atherosclerosis. PMID: 27079462
    47. Data show that cystatin C deposition in minimally affected samples was limited to the basement membrane (BM) between the dermis and epidermis. PMID: 28067897
    48. Urinary cystatin C levels in the acute kidney injury (AKI) preterm infant group were not significantly higher than in no-AKI group on day of life 1, 3 and 7. PMID: 27020372
    49. Serum cystatin C level was found to be associated with the development and worsening of AKI [Acute kidney injury ] in ICU [intensive care unit ] patients with sepsis. PMID: 28332367
    50. Elevated cystatin C levels at admission were independently associated with impaired myocardial perfusion, poor cardiac functional recovery and development of congestive heart failure in patients with anterior STEMI undergoing PCI. PMID: 26498756

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  • 相关疾病：
    Amyloidosis 6 (AMYL6); Macular degeneration, age-related, 11 (ARMD11)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Cystatin family
  • 组织特异性：
    Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ov
  • 数据库链接：

    HGNC: 2475

    OMIM: 105150

    KEGG: hsa:1471

    STRING: 9606.ENSP00000366124

    UniGene: Hs.304682