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  4. Human Creatine kinase M-type(CKM) ELISA kit

Human Creatine kinase M-type(CKM) ELISA kit

  • 中文名称：
    人M型肌酸激酶(CKM)酶联免疫试剂盒
  • 货号：
    CSB-EL005459HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CKM ELISA Kit was designed for the quantitative measurement of Human CKM protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.12 μU/mL-200 μU/mL and the sensitivity is 1.56 μU/mL.

  • 别名：
    CKM ELISA Kit; CKMM ELISA Kit; Creatine kinase M ELISA Kit; Creatine kinase M chain ELISA Kit; Creatine kinase M type ELISA Kit; Creatine kinase M-type ELISA Kit; Creatine kinase muscle ELISA Kit; Creatine kinase, muscle type ELISA Kit; KCRM_HUMAN ELISA Kit; M-CK ELISA Kit; MCK ELISA Kit; Muscle creatine kinase ELISA Kit
  • 缩写：
    CKM
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 μU/mL-200 μU/mL
  • 灵敏度：
    1.56 μU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 84    
    Range % 80-88    
    1:200 Average % 98    
    Range % 94-102    
    1:400 Average % 90    
    Range % 86-94    
    1:800 Average % 106    
    Range % 101-113    
  • 回收率：
    The recovery of human CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 90-100    
    EDTA plasma (n=4) 89 85-93    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    μU/ml OD1 OD2 Average      
    200 0.155 0.161 0.158      
    100 0.272 0.260 0.266      
    50 0.443 0.455 0.449      
    25 0.706 0.682 0.694      
    12.5 1.045 1.007 1.026      
    6.25 1.248 1.293 1.271      
    3.12 1.642 1.714 1.678      
    0 2.055 2.159 2.107      
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • 基因功能参考文献：
    1. The formation of insoluble aggregates would decrease levels of active Creatine kinase (CK) which may provide clues in CK deficiency disease. Moreover, these results indicated that the degree of synergism had closely relationship to the conformational changes of CK PMID: 28916380
    2. Low CK-MB expression is associated with ST-Segment Elevation Myocardial Infarction. PMID: 28100880
    3. On admission, high-sensitivity troponin T/creatine kinase-MB ratio was significantly higher in takotsubo syndrome patients compared to myocardial infarction patients. PMID: 28595746
    4. A genetic factor known to be associated with constitutive creatine kinase levels is also associated with creatine kinase variability and inducibility and the variant has an impact on inducibility of creatine kinase by trauma through a homozygous carrier. PMID: 28790154
    5. Marathoners with a lower CKMM response after the race had a more favorable polygenic profile than runners with high serum CKMM concentrations. This might suggest a significant role of genetic polymorphisms in the levels of exertional muscle damage and rhabdomyolysis. PMID: 28257486
    6. Myocardial Creatine Kinase is a significant independent predictor of 6-month left ventricle remodeling in myocardial infarction patients. PMID: 28138312
    7. A positive association was found between PANSS-total and sCK [serum creatine kinase] in SzA [schizoaffective disorder] and BP-I; however, PANSS-positive scores correlated with sCK only in SzA. Serum CK may serve as a biomarker for affective exacerbation rather than psychosis. PMID: 27086253
    8. These results demonstrate an association between physical performance measures and genetic variation in the muscle-specific creatine kinase gene (rs8111989). PMID: 26327553
    9. Muscle pain induced by simvastatin resulted in increased levels of creatine kinase compared to non-treated patients. PMID: 26874453
    10. Data show that a low serum creatine kinase activity is more common in female than male, and is associated with older age. PMID: 26738403
    11. CK-MB mass is more significant criteria of myocardial injury. PMID: 25802449
    12. troponin T and creatinine kinase isoenzyme (CK-MB) have roles in combined renal and myocardial injuries in asphyxiated infants PMID: 24625749
    13. The serum expression levels of myocardial creatine kinase and of galectin-3 reflect the physiopathology state of children with congenital heart defects after surgical correction. PMID: 26118042
    14. The variant rs11559024 in the CKM gene (Glu83Gly) was significantly associated with CK levels of statin users. PMID: 25214527
    15. elderly women classified as high response experienced greater serum response to eccentric resistance exercise PMID: 24189370
    16. Based on the obtained results, it may be speculated that the CKM A/G polymorphism is not an important determinant of endurance performance level in Polish and Russian rowers. PMID: 26027379
    17. Substantive creatine phosphokinase increases and rhabdomyolysis with statin use were particularly seen in patients starting treatment, those on large daily doses or interacting drugs or with larger numbers of concomitant drugs PMID: 24602118
    18. serum CK-MB elevated in underweight hemodialysis patients PMID: 24846126
    19. Asymptomatic hyper-CKemia is an uncommon association with hyponatremia of various etiologies. PMID: 24673369
    20. Cardiac enzyme elevations post-cardiac bypass or post-percutaneous coronary intervention are associated with an adverse long-term mortality; the causes of which are multifactorial. PMID: 23993326
    21. 3D mapping of the CK reaction rates and metabolic fluxes can be achieved in the skeletal muscle in vivo at relatively high spatial resolution and with acquisition times well tolerated by patients. PMID: 23436474
    22. High levels of blood creatine-kinase MB are associated with embolism source during acute phase of ischemic stroke. PMID: 22592287
    23. This study explored the relationship of cardiac function examined by echocardiography and serum creatine kinase (CK) and CK-MB levels with myocardial ischemia-reperfusion injury in a cohort of Chinese acute myocardial infarction patients. PMID: 21873942
    24. The crystal structure of CK indicates that the E79 and K138 interaction plays key roles in sustaining the recognition between N-terminal and C-terminal domains of muscle creatine kinase. PMID: 23274523
    25. The current enzymatic definition of procedural myocardial infarct (MI) (CK-myocardial band more than 3 times the upper limit of normal) used in clinical trials is less strongly associated with death than that of spontaneous MI. PMID: 23122801
    26. Creatine kinase MM TaqI and methylenetetrahydrofolate reductase C677T and A1298C gene polymorphisms influence exercise-induced C-reactive protein levels. PMID: 21706313
    27. Results indicate that these polymorphisms can indirectly influence performance, contribute to higher susceptibility to exercise-induced inflammation or protection against it, and perhaps affect future risks of CVD in athletes. PMID: 21516340
    28. Common variants of MSTN and CKM genes don't play a role in attaining high level endurance performance in Caucasians. PMID: 20536908
    29. Elevated creatine kinase-MB is associated with elevated white blood cell count and atherosclerotic plaque in patients with elective stent implantation. PMID: 20591515
    30. Cardiac troponin T and creatine kinase have roles in infarct size and left ventricular function after acute myocardial infarction PMID: 21448949
    31. Muscle-type creatine kinase physically interacts with the slow skeletal muscle-type MyBPC1 (myosin-binding protein C1). PMID: 21426302
    32. The overall efficacy of IMA [ischemia modified albumin]in differentiating AMI[Acute Myocardial Infarction] from Non-AMI cases appears to be comparable to that of CK-MB and cTnI PMID: 21456469
    33. two residues close to the dimer interface of MMCK were crucial for species-specific thermal stability PMID: 20558199
    34. The researchers found no association between the CK response after exercise and the presence of the CK-MM NcoI polymorphism. PMID: 20157874
    35. percutaneous coronary intervention induces temporal changes of P-selectin, Mg, and CK-MB, which may be involved in restenosis and ischemia-reperfusion injury, but not PAI-1 PMID: 20669347
    36. Elevated CK-MB values after elective angioplasty predicts reduced long-term event-free survival. PMID: 19670037
    37. The Dimension Vista cTnI, CK-MB, MYO, NTproBNP, and hsCRP methods demonstrate acceptable performance characteristics for use as an aid in the diagnosis and risk assessment of patients presenting with suspected acute coronary syndromes. PMID: 19523464
    38. A short version of the muscle creatine kinase promoter(MCK1350) allows for sustained dystrophin expression in skeletal muscle of newborn mdx mice. PMID: 11922612
    39. Results identify muscle-type creatine kinase as a binding partner of a central portion of myomesin and the closely related M-protein. PMID: 12972258
    40. The roles of Ile-69 and Val-325 are discussed in the delivery of both substrate specificity and catalysis of human muscle creatine kinase isozyme. PMID: 15504039
    41. In top-level professional cyclists capable of completing a classic 3-wk tour race, the frequency distribution of the D allele and the DD genotype seems to be higher than in other endurance athletes such as elite runners. PMID: 16037885
    42. Creatinine kinase-MB levels are elevated after adverse outcomes following percutaneous coronary interventions PMID: 16087811
    43. Elevated levels of creatinine kinase-MB after percutaneous coronary intervention are not a predictor of adverse outcomes PMID: 16092153
    44. first monomeric intermediate captured during refolding of muscle-type creatine kinase; s propose that aggregation is caused by interaction between such monomeric intermediates. PMID: 16373479
    45. CK-MM autoantibodies can modulate the rate of CK clearance from the circulation in myositis PMID: 16810680
    46. The results show conclusively that, Cys283 is not responsible for the pKa of 5.4 observed in the WT V/K(creatine) pH profile. PMID: 16981706
    47. generation of O-CK is a negative regulation of R-CK and O-CK might play essential roles in the molecular turnover of MM-CK PMID: 17303563
    48. CK-MM AA genotype and percent body fat may be part of the constellation of mechanisms that explain susceptibility to exertional rhabdomyolysis. PMID: 17478608
    49. Indicate a positive association of the ACE ID genotype with creatine kinase response to strenuous exercise. Genotype may determine risk for developing muscle damage. PMID: 17885020
    50. Muscle type CK elevation in hypertrabeculation/noncompaction suggests neuromuscular disorder and prompts neurological investigations. PMID: 18055044

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    ATP:guanido phosphotransferase family
  • 数据库链接：

    HGNC: 1994

    OMIM: 123310

    KEGG: hsa:1158

    STRING: 9606.ENSP00000221476

    UniGene: Hs.334347