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  4. Human Complement fragment 3c,C3c ELISA Kit

Human Complement fragment 3c,C3c ELISA Kit

  • 中文名称：
    人补体片段3c(C3c)酶联免疫试剂盒
  • 货号：
    CSB-E11168h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human C3c ELISA Kit was designed for the quantitative measurement of Human C3c protein in serum, plasma, cell culture supernates, urine, saliva. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

  • 缩写：
    C3c
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, urine, saliva
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C3c in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5000Average %88
    Range %84-92
    1:10000Average %94
    Range %90-98
    1:20000Average %99
    Range %95-104
    1:40000Average %102
    Range %96-108
  • 回收率：
    The recovery of human C3c spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8993-96
    EDTA plasma (n=4)10095-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.266 2.345 2.306 2.174
    201.946 1.973 1.960 1.828
    101.510 1.546 1.528 1.396
    51.185 1.198 1.192 1.060
    2.50.854 0.814 0.834 0.702
    1.250.521 0.501 0.511 0.379
    0.6250.335 0.325 0.330 0.198
    00.131 0.133 0.132 　
  • 数据处理：
  • 货期：
    3-5 working days

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