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  4. Human Complement C1s subcomponent(C1S) ELISA kit

Human Complement C1s subcomponent(C1S) ELISA kit

  • 中文名称：
    人补体成分C1s(C1s)酶联免疫试剂盒
  • 货号：
    CSB-EL003657HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human C1S ELISA Kit was designed for the quantitative measurement of Human C1S protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 ng/mL-1600 ng/mL and the sensitivity is 6.25 ng/mL.

  • 别名：
    Basic proline rich peptide IB 1 ELISA Kit; C1 esterase ELISA Kit; C1S ELISA Kit; C1S_HUMAN ELISA Kit; Complement C1s subcomponent ELISA Kit; Complement C1s subcomponent heavy chain ELISA Kit; Complement C1s subcomponent light chain ELISA Kit; Complement component 1 s subcomponent ELISA Kit; Complement component 1 subcomponent s ELISA Kit; FLJ44757 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    25 ng/mL-1600 ng/mL
  • 灵敏度：
    6.25 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C1S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 100    
    Range % 91-104    
    1:200 Average % 107    
    Range % 98-110    
    1:400 Average % 88    
    Range % 82-93    
    1:800 Average % 96    
    Range % 87-99    
  • 回收率：
    The recovery of human C1S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 110 98-114    
    EDTA plasma (n=4) 98 90-102    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    1600 2.740 2.685 2.713 2.598    
    800 2.346 2.276 2.311 2.196    
    400 1.488 1.454 1.471 1.356    
    200 0.756 0.768 0.762 0.647    
    100 0.396 0.411 0.404 0.289    
    50 0.246 0.239 0.243 0.128    
    25 0.162 0.170 0.166 0.051    
    0 0.113 0.117 0.115 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

***Please let us know the active ingredient of the stop solution and its
concentration***
***Please let us know the type, concentration, and state
(liquid/lyophilized) of the preservatives used*** ***Please let us know the
expression host of the standard***

A:
Thanks for your inquiry!
Pls check our information:
CSB-EL003657HU
Stop Solution is 4N sulfuric acid
Preservatives is liquid porclin 300(1:3000);
Standard is 293 cell-derived recombinant protein.

靶点详情

  • 功能：
    C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
  • 基因功能参考文献：
    1. TNT003, an inhibitor of the serine protease C1s, prevents complement activation induced by cold agglutinins. PMID: 24695853
    2. Data indicate that complement C1s mRNA level was low in ICR-derived glomerulonephritis (ICGN) mice liver as compared with age-matched ICR mice. PMID: 23989031
    3. A molecular switch governs the interaction between the human complement protease C1s and its substrate, complement C4. PMID: 23592783
    4. Four positively charged amino acids on the serine protease domain appear to form a catalytic exosite that is required for efficient cleavage of C4 in the classical pathway of complement. PMID: 22855709
    5. Detailed mapping of post-translational modifications and insights into the C1r/C1s binding sites. PMID: 20008834
    6. Interaction with the prime side residues at the cleavage point in C1s enhances the affinity of the enzyme for complement 2 and complement 4 substrates; these prime subsite residues mediate positive cooperativity in the cleavage of the substrate. PMID: 14674770
    7. full specificity of the enzyme using a randomized phage display library PMID: 16169853
    8. There are splice variants of C1s mRNA transcripts in normal human cells PMID: 18062908

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  • 相关疾病：
    Complement component C1s deficiency (C1SD); Ehlers-Danlos syndrome, periodontal type, 2 (EDSPD2)
  • 蛋白家族：
    Peptidase S1 family
  • 数据库链接：

    HGNC: 1247

    OMIM: 120580

    KEGG: hsa:716

    STRING: 9606.ENSP00000328173

    UniGene: Hs.458355