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  4. Human Collagen triple helix repeat-containing protein 1(CTHRC1) ELISA kit

Human Collagen triple helix repeat-containing protein 1(CTHRC1) ELISA kit

  • 中文名称：
    人胶原蛋白三螺旋重复包含蛋白1(CTHRC1)酶联免疫试剂盒
  • 货号：
    CSB-EL006162HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The human collagen triple helix repeat-containing protein 1 (CTHRC1) ELISA Kit is used to quantitatively measure human CTHRC1 concentrations in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of CTHRC1 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

    CTHRC1 is a secreted extracellular matrix (ECM) protein transiently expressed in the repair process after arterial injury and myocardial infarction, thus regulating collagen matrix deposition and cell migration and promoting tissue repair through the TGF-β signaling pathway. It is also involved in some pathophysiological processes, including increasing bone mass, preventing myelination, and reversing collagen synthesis in many tumor cells. Increased expression of CTHRC1 is associated with tumorigenesis, proliferation, invasion, and metastasis in various types of human cancer, including gastric cancer, pancreatic cancer, breast cancer, colorectal cancer, non-small-cell lung carcinoma, and melanoma.

  • 别名：
    Collagen triple helix repeat containing 1 ELISA Kit; Collagen triple helix repeat containing protein 1 ELISA Kit; Collagen triple helix repeat-containing protein 1 ELISA Kit; CTHR1_HUMAN ELISA Kit; Cthrc1 ELISA Kit; NMTC1 protein ELISA Kit; Protein NMTC1 ELISA Kit
  • 缩写：
    CTHRC1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 ng/mL-400 ng/mL
  • 灵敏度：
    1.56 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CTHRC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %80-89
    1:2Average %91
    Range %88-96
    1:4Average %95
    Range %91-99
    1:8Average %93
    Range %87-96
  • 回收率：
    The recovery of human CTHRC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9992-104
    EDTA plasma (n=4)9289-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    4002.445 2.372 2.409 2.217
    2001.872 1.950 1.911 1.719
    1001.401 1.418 1.410 1.218
    500.892 0.863 0.878 0.686
    250.585 0.544 0.565 0.373
    12.50.403 0.389 0.396 0.204
    6.250.292 0.279 0.286 0.094
    00.194 0.190 0.192 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    May act as a negative regulator of collagen matrix deposition.
  • 基因功能参考文献：
    1. CTHRC1 serves as a pro-metastatic gene that contributes to NSCLC invasion and metastasis, which are mediated by upregulated MMP7 and MMP9 expression. Targeting CTHRC1 may be beneficial for inhibiting NSCLC metastasis. PMID: 29631554
    2. High Collagen Triple Helix Repeat Containing 1 Expression is associated with Osteosarcoma. PMID: 29970438
    3. E6/E7-p53-POU2F1-CTHRC1 axis promotes cervical cancer cell invasion and metastasis PMID: 28303973
    4. IL-1beta and CTHRC1 are upregulated in patients with Osteoarthritis. PMID: 29393342
    5. CTHRC1 interacts with integrin beta3 and accelerates the FAK phosphorylation to promote ovarian cancer cell adhesion, migration and invasion in vitro and in vivo. PMID: 29021002
    6. CTHRC1 plays a pivotal role in a great many fields, including increases bone mass, prevents myelination, reverses collagen synthesis in keloid fibroblasts, and increases fibroblast-like synoviocytes migration speed and abundant production of arthritic pannus in rheumatoid arthritis PMID: 28901303
    7. CTHRC1, negatively regulated by miR-30c, promoted cell proliferation, invasion and migration and suppressed cell apoptosis in breast cancer, which might be by activating GSK-3beta/beta-catenin signaling and inhibiting Bax/Caspase-9/Caspase-3 signaling respectively. PMID: 28697793
    8. Our data suggest that CTHRC1 may act as an oncogenic driver in progression and metastasis of ESCC, and may serve as a potential biomarker for prognosis and personalized therapy. PMID: 28645305
    9. The negative and sensitivity-predictive values of CTHRC1 staining were excellent for both lymph node and peritoneal metastases. PMID: 27870703
    10. High CTHRC1 expression is associated with metastatic melanomas. PMID: 26918341
    11. CTHRC1 was established as a novel marker of activated synoviocytes in murine experimental arthritis and rheumatoid arthritis PMID: 27430622
    12. ANOS1 and its co-expression partner, CTHRC1, promote the development and metastasis of colorectal cancer. PMID: 28854193
    13. Expression of CTHRC1 was significantly higher in Wilms' tumor compared to the expression in the adjacent non-cancerous tissues. High tumor expression of CTHRC1 was associated with tumor size, clinical stage, histopathological type, and vascular invasion/metastasis. Patients with high CTHRC1 expression also exhibited a shorter survival. PMID: 27230801
    14. CTHRC1 expression is significantly upregulated in human masticatory mucosa during wound healing. PMID: 28005267
    15. CTHRC1 downregulation inhibited proliferation. PMID: 28281968
    16. The knockdown of CTHRC1 exerts inhibitory effects on the proliferation and migration ability of glioblastoma cells. PMID: 28277194
    17. CTHRC1 may play a role in the progression of ovarian cancer. PMID: 27779718
    18. this study shows that the serum CTHRC1 level was significantly higher in the influenza A virus infection patients than in the healthy individuals; the influenza A virus non-structural protein NS1 upregulates the expression of CTHRC1 protein PMID: 27718266
    19. Gene expression levels of three randomly selected DEGs, VCAN, COL5A1 and KCNJ16, were examined using RT-PCR in 10 ATC samples.. angiogenesis was activated by the high expression of CTHRC1, VCAN and POSTN, providing necessary nutrition for tumor cells PMID: 27599582
    20. increased CTHRC1 expression is associated with advanced TNM stage, increased LN metastasis and tumor size, and decreased OS and DFS, indicating that CTHRC1 may be a biomarker for prognosis of cancer patients PMID: 27323076
    21. High CTHRC1 expression is associated with Osteosarcoma. PMID: 27043295
    22. s showed that ectopic transfection of CTHRC1 in EOC cells up-regulated the expression of EMT markers such as N-cadherin and vimentin, and EMT-associated transcriptional factor Snail. PMID: 26452130
    23. CTHRC1 is secreted both by colorectal epithelia cells and stromal fibroblasts. CTHRC1 overexpression promotes colorectal cancer cell migration, invasion and proliferation in vitro. PMID: 26722469
    24. determined the mRNA and protein expression of CTHRC1 in oral squamous cell carcinoma and evaluated the clinical and prognostic impact of CTHRC1 overexpression PMID: 26664254
    25. CTHRC1 has a novel role in viral infection. PMID: 26180054
    26. HBV facilitates HCC development through activating the oncoprotein CTHRC1. PMID: 25263696
    27. CTHRC1 was overexpressed in human non-small cell lung cancer tissues and non-small cell lung cancer cell lines at the protein and mRNA level. PMID: 25238260
    28. let-7b may directly target Cthrc1 and function as a tumor suppressor gene in gastric cancer. PMID: 25510669
    29. CTHRC1 acts as a prognostic factor and promotes invasiveness of gastrointestinal stromal tumors by activating Wnt/PCP-Rho signaling. PMID: 24726140
    30. Cthrc1 overexpression was associated with non-small cell lung cancers. PMID: 25139095
    31. Cthrc1 is a pituitary hormone with significantly elevated levels in subjects carrying variant alleles of the melanocortin-1 receptor as wells as in patients with inflammatory conditions. PMID: 24945147
    32. CTHRC1 has the potential to be a new biomarker for the aggressive hepatocellular carcinoma PMID: 24841500
    33. CTHRC1 expression is elevated in human colon cancer cell lines and clinical specimens, and promotes cancer cell invasivity through ERK-dependent induction of MMP9 expression. PMID: 24504172
    34. Studied CTHRC1 expression in human breast cancer tissue. A significant increase of CTHRC1 mRNA expression was seen in breast cancer tissue compared to the normal tissue from the same patients using RT-PCR and real-time PCR. PMID: 23658133
    35. Overexpression of CTHRC1 in hepatocellular carcinoma promotes tumor invasion and predicts poor prognosis. PMID: 23922981
    36. Rs35500845 in the CTHRC1 gene was associated with Paget's disease of bone in the French-Canadian population. PMID: 24370779
    37. high Cthrc1 expression was an independent prognostic factor for both overall survival and disease-free survival of patients with gastric carcinoma PMID: 24746208
    38. Data suggest that in oral squamous cell carcinoma (OSCC), dysregulation of canonical Wnt signaling and DPAGT1-dependent N-glycosylation induces CTHRC1, thereby driving OSCC cell migration and tumor spread. PMID: 23703614
    39. this is the first demonstration of Cthrc1 as a marker of the severity of the disease progression in the dystrophic muscles. PMID: 23274062
    40. the results of our study suggest that increased expression of CTHRC1 is associated with peritoneal carcinomatosis in Colorectal cancer patients PMID: 23359115
    41. CTHRC1 has a role in pancreatic cancer progression and metastasis by regulating migration and adhesion activities of cancer cells. PMID: 23222813
    42. Data indicate that the upregulated expression of collagen triple helix repeat containing 1 (CTHRC1) in gastric carcinogenesis contributes to tumor cell invasion and metastasis. PMID: 22590977
    43. Three major genes, MSR1, ASCC1, and CTHRC1 were associated with Barrett esophagus/esophageal adenocarcinoma PMID: 21791690
    44. CTHRC1 is transiently expressed in the arterial wall in response to injury where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration. PMID: 15618538
    45. Aberrant expression of CTHRC1 is widely present in human solid cancers and seems to be associated with cancer tissue invasion and metastasis. PMID: 16778098
    46. Intracellular localization of Cthrc1 characterizes differentiated smooth muscle. PMID: 18467647

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  • 相关疾病：
    Barrett esophagus (BE)
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Isoform 1 is expressed in calcified atherosclerotic plaque and chondrocyte-like cells.
  • 数据库链接：

    HGNC: 18831

    OMIM: 610635

    KEGG: hsa:115908

    STRING: 9606.ENSP00000330523

    UniGene: Hs.405614