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  4. Human Collagen alpha-2(I) chain (COL1A2) ELISA kit

Human Collagen alpha-2(I) chain (COL1A2) ELISA kit

  • 中文名称：
    人I型胶原α-2链(COL1A2)酶联免疫试剂盒
  • 货号：
    CSB-EL005728HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human COL1A2 ELISA Kit was designed for the quantitative measurement of Human COL1A2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    Alpha 2 collagen type I ELISA Kit; Alpha 2 type I collagen ELISA Kit; Alpha 2 type I procollagen ELISA Kit; Alpha 2(I) collagen ELISA Kit; Alpha 2(I) procollagen ELISA Kit; Alpha-2 type I collagen ELISA Kit; CO1A2_HUMAN ELISA Kit; COL1A2 ELISA Kit; Collagen alpha 2(I) chain ELISA Kit; Collagen alpha-2(I) chain ELISA Kit; Collagen I alpha 2 polypeptide ELISA Kit; Collagen of skin tendon and bone alpha 2 chain ELISA Kit; Collagen type I alpha 2 ELISA Kit; OI4 ELISA Kit; Osteogenesis imperfecta type IV ELISA Kit; Type I procollagen ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human COL1A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %82-93
    1:2Average %98
    Range %94-102
    1:4Average %102
    Range %98-106
    1:8Average %95
    Range %90-100
  • 回收率：
    The recovery of human COL1A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9185-96
    EDTA plasma (n=4)10498-110
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.549 2.635 2.592 2.475
    102.113 2.244 2.179 2.062
    51.427 1.486 1.457 1.340
    2.50.899 0.931 0.915 0.798
    1.250.533 0.511 0.522 0.405
    0.6250.364 0.342 0.353 0.236
    0.3120.227 0.218 0.223 0.106
    00.113 0.121 0.117
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • 基因功能参考文献：
    1. Osteogenesis imperfecta with ectopic mineralizations in dentin and cementum and a COL1A2 mutation has been reported in a Thai adult patient and his preschool daughter. PMID: 29636545
    2. This study showed that there is no association of collagen type-(2a) and intracranial aneurysms. PMID: 29164999
    3. High COL1A2 expression is associated with Gastric Cancer. PMID: 28401451
    4. The aim of this study is to determine whether EcoRI, Del38 and PvuII polymorphisms of COL1A2 are associated with the development of osteoporosis and osteopenia in post-menopausal Polish women. The analyzed COL1A2 polymorphisms seem to be related to osteoporosis development. The COL1A2 polymorphism may be a genetic risk factor related to the development of osteoporosis. PMID: 28930368
    5. COL1A2 gene mutation is associated with osteogenesis imperfecta. PMID: 28810924
    6. We evaluated the association of a 7-base pair (7-bp) indel polymorphism (rs3917) in the 3'UTR of COL1A2 with the risk of sudden cardiac death in a Chinese population. Our data provided initial evidence that rs3917 was highly relevant to SCD susceptibility, and this indel may become a potential marker for molecular diagnosis and genetic counseling of SCD. PMID: 28738217
    7. meta-analysis suggests COL1A2 rs42524 is a significant risk factor for Intracranial Aneurysm susceptibility. PMID: 28671939
    8. By acting probably as a posttranscriptional regulator with a different efficacy on COL2A1 and COL1A2 expression, miR-29b can contribute to the collagens imbalance associated with an abnormal chondrocyte phenotype. PMID: 28612031
    9. High methylation of COL1A2 is associated with head and neck cancer. PMID: 27027429
    10. a phenotypically distinctive group of OI is caused by a mono-allelic COL1 C-propeptide cleavage site mutations and biallelic BMP1 mutations. The subgroup is characterized by high bone mass and other unique skeletal changes, such as coarse trabeculae, pseudo-fractures, and metaphyseal constriction PMID: 27264419
    11. We suggest that the mutation p.G337C in the COL1A2 gene is pathogenic for osteogenesis imperfecta by affecting the protein structure and the function of collagen. PMID: 28953610
    12. Identify PDGFRbeta as a driver in activating Akt/mTORC1 nexus for high glucose-mediated expression of collagen I (alpha2) in proximal tubular epithelial cells, which contributes to tubulointerstitial fibrosis in diabetic nephropathy. PMID: 28424212
    13. Flightless-I (Drosophila) homolog (FLII) activates TGFbeta1-mediated expression of COL1A2 gene. PMID: 25451260
    14. In the individuals with a COL1A2 mutation, 80% (8/10) of those with a glycine substitution located C terminal of p.Gly211 exhibited DGI in both dentitions while no individual (0/5) with a mutation N-terminal of this point (p = 0.007) exhibited DGI in either dentition. PMID: 28498836
    15. COL1A2 expression was positively related to tumor size, depth of invasion, and to lower overall survival of gastric cancer patients. PMID: 27894325
    16. the present study demonstrated that the INS/DEL polymorphism in the 3'UTR of COL1A2 is able to interfere with the interaction between miRNA and mRNA. In addition, it is the first study, to the best of our knowledge, to indicate that the minor allele (Del) is associated with a reduced risk of developing osteoporosis. PMID: 27665867
    17. THBS2, but not COL1A2 and SPP1, may serve as an indicator of gastric cancer prognosis. PMID: 27997896
    18. In children aged 6-12, the presence of polymorphism in the COL1A2 gene was not associated with the severity of dental fluorosis. PMID: 26564713
    19. Study suggests that the COL1A2 rs42524 polymorphism is associated with the development of hypertensive intracerebral hemorrhage, particularly in conjunction with tobacco use and alcohol consumption. PMID: 26910001
    20. Two Rare Mutations in the COL1A2 Gene Associate With Low Bone Mineral Density and Fractures in Iceland. PMID: 26235824
    21. COL1A2 mutation is associated with atypical osteogenesis imperfect. PMID: 26471105
    22. analysis of COL1A2 (type I collagen) polymorphisms in the central-eastern Mediterranean Basin PMID: 26065693
    23. Severe osteogenesis imperfecta caused by double glycine substitutions near the amino-terminal triple helical region in COL1A2. PMID: 25858481
    24. These data indicate a novel functional role of AnxA2 in the negative post-transcriptional regulation of type I collagen synthesis in human fibroblasts. PMID: 25290763
    25. The novel c.946G>T/p.G316C mutation in COL1A2 gene is associated with with osteogenesis imperfecta type I. PMID: 25608812
    26. Extracellular matrix proteins expression profiling in chemoresistant variants of the A2780 ovarian cancer cell line. PMID: 24804215
    27. Data indicate that the IL-33/ST2 protein pathway may promote airway remodeling in asthma through activating HLF-1 fibroblast to over-express fibronectin 1 (FN1) and type 1 collagen (Col1). PMID: 25200162
    28. The COL1A2 gene is associated with intracranial aneurysms in a subset of the German population, but it is not responsible for the majority of aneurysms. PMID: 23800505
    29. these results indicate that under quiescent conditions Fli1 recruits HDAC1/p300 to the COL1A2 promoter and suppresses the expression of the COL1A2 gene by chromatin remodeling through histone deacetylation. PMID: 24058639
    30. this study highlights an essential role for Sin3B in IFN-c induced COL1A2 repression in smooth muscle cells. PMID: 24709079
    31. Missense or nonsense mutations in COL1A2 were identified in 2 unrelated patients with osteigenesis imperfecta. PMID: 24140640
    32. Expression of COL1A2 mRNA encoding key fibrotic extracellular matrix molecules was down-regulated by pre-miRNA-29b. PMID: 24641356
    33. Increased degradation of collagen type I was observed in the supernatant of KFs expressing siTIMP-1, but not siTIMP-2, with the suppression of cell viability and induction of apoptosis. PMID: 24042342
    34. Data suggest that common genetic variations in COL1A2 may influence chronic venous insufficiency risk, possibly through microRNA-382-mediated regulation. PMID: 23849651
    35. Family study of missense mutation of c.2279G>A, Gly760Glu in exon 37 of COL1A2 causing familial osteogenesis imperfecta type IV. PMID: 23548243
    36. We conclude that the COL1A2 rs42524 polymorphism could be a genetic risk factor for primary intracerebral hemorrhage among Han Chinese. PMID: 23036172
    37. A spontaneous novel mutation in COL1A2 (c.1171G>A; p.Gly391Ser) causing only dentin defects and a novel mutation in PAX9 (c.43T>A; p.Phe15Ile) causing hypodontia were identified and correlated with the phenotypic presentations in the family. PMID: 23227268
    38. these data have identified as novel pathway whereby SIRT1 maintains COL1A2 synthesis in SMCs by modulating RFX5 activity. PMID: 23079621
    39. found no evidence supporting the putative link of COL1A2 alleles with otosclerosis. PMID: 22130917
    40. Scleraxis thus appears to play a key role in the transcriptional regulation of type I collagen synthesis. PMID: 22796342
    41. The rs42524 polymorphism in COL1A2 is a risk allele for neovascular age-related macular degeneration (nAMD) in a Han Chinese population. PMID: 22815632
    42. Data suggest that the Collagen alpha2 (I) (COL1A2) gene may play a role in the tumorigenesis of head and neck squamous cell carcinoma (HNSCC) and may serve as an important biomarker. PMID: 22674299
    43. TGF-beta stimulation upregulated type I collagen expression via miR-196a downregulation in normal fibroblasts. Cotransfection of the 196a protector blocked the miR-196a inhibitor-mediated upregulation of a2(I) collagen. PMID: 22379029
    44. Matrix-mediated downregulation of type I alpha 2 collagen occurs zia an Sp1 transcription factor Pathway PMID: 22131293
    45. transcript levels of UCHL1, COL1A2, THBS1 and TNFRSF10D were inversely correlated with promoter methylation PMID: 22028813
    46. MRTF-A is an important regulator of collagen synthesis in lung fibroblasts and exhibits a dependence on both SRF and Sp1 function to enhance collagen expression PMID: 22049076
    47. The patient with COL1A2 Ala1119Thr mutation has mild osteogenesis imperfecta. PMID: 21344539
    48. This study identifies two novel mutations p.G1102A and p.Y1117C that cause osteogenesis imperfecta PMID: 21530898
    49. Genetic variation within the COL1A2 gene is associated with increased risk of hepatocellular carcinoma in a Chinese population. PMID: 21665180
    50. COL10A2 is upregulated in gastric adenocarcinoma and is associated with tumor progression. PMID: 21443102

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  • 相关疾病：
    Ehlers-Danlos syndrome 7B (EDS7B); Osteogenesis imperfecta 1 (OI1); Osteogenesis imperfecta 2 (OI2); Ehlers-Danlos syndrome, autosomal recessive, cardiac valvular form (EDSCV); Osteogenesis imperfecta 3 (OI3); Osteogenesis imperfecta 4 (OI4)
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Fibrillar collagen family
  • 组织特异性：
    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • 数据库链接：

    HGNC: 2198

    OMIM: 120160

    KEGG: hsa:1278

    STRING: 9606.ENSP00000297268

    UniGene: Hs.489142