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  4. Human Cathelicidin antimicrobial peptide(CAMP) ELISA kit

Human Cathelicidin antimicrobial peptide(CAMP) ELISA kit

  • 中文名称：
    人抗菌肽(CAMP)酶联免疫试剂盒
  • 货号：
    CSB-EL004476HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 别名：
    CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37,
  • 缩写：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.45 ng/mL-30 ng/mL
  • 灵敏度：
    0.11 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 88  
    Range % 80-92  
    1:200 Average % 96  
    Range % 91-105  
    1:400 Average % 99  
    Range % 92-110  
    1:800 Average % 95  
    Range % 86-98  
  • 回收率：
    The recovery of human CAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 89-98  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    30 2.713 2.664 2.689 2.612  
    15 1.687 1.567 1.627 1.550  
    7.5 1.047 0.989 1.018 0.941  
    3.75 0.563 0.543 0.553 0.476  
    1.8 0.339 0.324 0.332 0.255  
    0.9 0.206 0.196 0.201 0.124  
    0.45 0.145 0.139 0.142 0.065  
    0 0.080 0.074 0.077 　  
  • 数据处理：
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

we are interested in measurements of the Cathelicidin antimicrobial peptide (CAMP) in human fecal samples. According to your homepage, your assay is suitable for blood samples or tissue homogenates. But have you ever tested it in fecal samples? Would you expect it to work using fecal samples?

A:
Thanks for your inquiry!
CSB-EL004476HU
We tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test human fecal samplee ,we suggest you do a pretest . Pls find following sample preparation method.
Feces Dilute samples with appropriate Sample Diluent. This can be achieved by adding 0.1g sample to appropriate Sample Diluent. Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.
Pls let me know if you have any further questions. Thank you.