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  4. Human Cartilage acidic protein 1(CRTAC1) ELISA kit

Human Cartilage acidic protein 1(CRTAC1) ELISA kit

  • 中文名称：
    人软骨酸性蛋白1(CRTAC1)酶联免疫试剂盒
  • 货号：
    CSB-EL005997HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CRTAC1 ELISA Kit was designed for the quantitative measurement of Human CRTAC1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.68 ng/mL-300 ng/mL and the sensitivity is 1.17 ng/mL.

  • 别名：
    68 kDa chondrocyte expressed protein ELISA Kit; 68 kDa chondrocyte-expressed protein ELISA Kit; Acidic secreted protein in cartilage ELISA Kit; ASPIC ELISA Kit; ASPIC1 ELISA Kit; Cartilage acidic protein 1 ELISA Kit; Cartilage acidic protein 1 precursor ELISA Kit; CEP 68 ELISA Kit; CEP-68 ELISA Kit; CEP68 ELISA Kit; Chondrocyte expressed protein 68 kDa CEP 68 ELISA Kit; CRAC1_HUMAN ELISA Kit; CRTAC1 ELISA Kit; Protein CRTAC1-B ELISA Kit; W307 ELISA Kit
  • 缩写：
    CRTAC1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    4.68 ng/mL-300 ng/mL
  • 灵敏度：
    1.17 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CRTAC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %91
    Range %87-96
    1:10Average %97
    Range %93-101
    1:20Average %102
    Range %99-103
    1:40Average %88
    Range %86-91
  • 回收率：
    The recovery of human CRTAC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-93
    EDTA plasma (n=4)10298-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    3002.522 2.735 2.629 2.444
    1502.043 2.102 2.073 1.888
    751.317 1.315 1.316 1.131
    37.50.875 0.857 0.866 0.681
    18.750.470 0.445 0.458 0.273
    9.370.366 0.380 0.373 0.188
    4.680.254 0.252 0.253 0.068
    00.186 0.184 0.185
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 基因功能参考文献：
    1. Confirm the increase of CRTAC1 in cartilage biopsies from OA patients undergoing joint replacement by real-time PCR and immunohistochemistry. Furthermore, we report that proinflammatory cytokines interleukin-1beta and tumor necrosis factor alpha upregulate CRTAC1 expression in primary articular chondrocytes and synovial fibroblasts. PMID: 27415616
    2. It was shown for the first time the propensity of CRTAC1 to form amyloid-like structures, and it was hypothesized that the aggregating property of CRTAC1 may be related to its disease-association. PMID: 27862299
    3. These results provided new insights into the mechanism of cataract development, and demonstrated that CRTAC1 could be a potentially novel target for cataract treatment. PMID: 27855397
    4. CRTAC1 acquired an alternate last exon from the tail-to-tail oriented neighbouring gene in humans resulting in the glycosylated isoform CRTAC1-A which represents a new extracellular matrix molecule of articular cartilage. PMID: 17074475
    5. in bone fracture patients, 12 proteins were related to bone/cartilage metabolism, including: TGF-beta induced protein IG-H(3), cartilage acidic protein 1, procollagen C proteinase enhancer protein and TGF-beta receptor III. PMID: 17602227

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Expressed in the interterritorial matrix of articular deep zone cartilage (at protein level). Isoform 1 and isoform 2 are expressed in brain. Isoform 1 is detected in lung and chondrocytes. Detected in cartilage, bone, cultured chondrocytes and lung, and
  • 数据库链接：

    HGNC: 14882

    OMIM: 606276

    KEGG: hsa:55118

    STRING: 9606.ENSP00000359629

    UniGene: Hs.500736