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  4. Human Carbonic Anhydrase 3(CA3) ELISA Kit

Human Carbonic Anhydrase 3(CA3) ELISA Kit

  • 中文名称：
    人碳酸酐酶3(CA3)酶联免疫试剂盒
  • 货号：
    CSB-E15962h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CA3 ELISA Kit was designed for the quantitative measurement of Human CA3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL.

  • 别名：
    CA III ELISA Kit; CA-III ELISA Kit; Ca3 ELISA Kit; CAH3_HUMAN ELISA Kit; CAIII ELISA Kit; Car3 ELISA Kit; Carbonate dehydratase III ELISA Kit; Carbonic anhydrase 3 ELISA Kit; Carbonic anhydrase III ELISA Kit; Carbonic anhydrase III muscle specific ELISA Kit; EC 4.2.1.1 ELISA Kit; Epididymis secretory sperm binding protein Li 167mP ELISA Kit; HEL S 167mP ELISA Kit
  • 缩写：
    CA3
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 ng/mL-500 ng/mL
  • 灵敏度：
    1.95 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-96  
    1:2 Average % 95  
    Range % 90-105  
    1:4 Average % 103  
    Range % 97-107  
    1:8 Average % 89  
    Range % 85-95  
  • 回收率：
    The recovery of human CA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-96  
    EDTA plasma (n=4) 97 90-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    500 2.748 2.579 2.664 2.512  
    250 2.187 2.136 2.162 2.010  
    125 1.467 1.488 1.478 1.326  
    62.5 0.897 0.722 0.810 0.658  
    31.2 0.488 0.490 0.489 0.337  
    15.6 0.346 0.345 0.346 0.194  
    7.8 0.225 0.237 0.231 0.079  
    0 0.156 0.148 0.152    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Reversible hydration of carbon dioxide.
  • 基因功能参考文献：
    1. CAIII and Hsp70 expressions were higher in LPRD patients that in non-LPRD patients, suggesting the possibility as one of biomomarker in LPRD diagnosis. PMID: 26847302
    2. this study detected the formation and colocalization of 6-nitrotryptophan-containing proteins and CAIII in the skin of atopic dermatitis patients, not only in the lesional part but also in the nonlesional part, through histochemical analyses PMID: 24838180
    3. Report that anti-CAIII antibodies may be useful for diagnosing microscopic polyangiitis. PMID: 23981757
    4. The carbonic anhydrase CA3 isoform displayed an increased abundance during muscle aging, which was independently verified by immunoblotting of differently aged human skeletal muscle samples. PMID: 22797148
    5. The generation of CA III and IV autoantibodies, antioxidant enzymes, and cytokines might influence each other. PMID: 23049597
    6. Crystallization and preliminary X-ray analysis of human carbonic anhydrase III PMID: 11976500
    7. Data show that inserting histidine residues into the active site cavity of carbonic anhydrase II or III results in rates of proton transfer to the zinc-bound hydroxide that are antagonistic or suppressive with respect to the corresponding single mutants. PMID: 12171926
    8. Proton transfer within the active-site cavity of carbonic anhydrase III PMID: 12484342
    9. myoglobin/carbonic anhydrase III ratio in the blood proved to be a more specific indicator for myocardial damage than myoglobin alone after myocardial infarction. PMID: 12745799
    10. CAIII expression is upregulated in kidney cortex samples from the end-stage kidney of a patient with Dent's disease owing to the G506E mutation of CLCN5 PMID: 18322545
    11. CAIII promotes transformation and invasion capability in hepatoma cells through FAK signaling pathway. PMID: 18444244
    12. The level of CAIII is specifically insufficient in the skeletal muscle of myasthenia gravis patients. PMID: 19301202

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Alpha-carbonic anhydrase family
  • 组织特异性：
    Muscle specific.
  • 数据库链接：

    HGNC: 1374

    OMIM: 114750

    KEGG: hsa:761

    STRING: 9606.ENSP00000285381

    UniGene: Hs.82129