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Human Calponin-1(CNN1)ELISA Kit

  • 中文名称：
    人调宁蛋白- 1(CNN1)酶联免疫试剂盒
  • 货号：
    CSB-E14109h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CNN1 ELISA Kit was designed for the quantitative measurement of Human CNN1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    Basic calponin ELISA Kit; Calponin 1 ELISA Kit; Calponin 1 basic smooth muscle ELISA Kit; Calponin H1 ELISA Kit; Calponin H1 smooth muscle ELISA Kit; Calponin-1 ELISA Kit; Calponin1 ELISA Kit; Calponins basic ELISA Kit; CNN 1 ELISA Kit; Cnn1 ELISA Kit; CNN1_HUMAN ELISA Kit; Epididymis secretory protein Li 14 ELISA Kit; HEL S 14 ELISA Kit; Sm Calp ELISA Kit; SMCC ELISA Kit; smooth muscle ELISA Kit
  • 缩写：
    CNN1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of Human CNN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %84-92
    1:2Average %95
    Range %90-99
    1:4Average %94
    Range %85-99
    1:8Average %104
    Range %98-108
  • 回收率：
    The recovery of Human CNN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-98
    EDTA plasma (n=4)8783-94
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.255 2.345 2.300 2.202
    51.715 1.756 1.736 1.638
    2.51.064 1.024 1.044 0.946
    1.250.523 0.515 0.519 0.421
    0.6250.334 0.324 0.329 0.231
    0.3120.251 0.234 0.243 0.145
    0.1560.162 0.159 0.161 0.063
    00.097 0.098 0.098
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
  • 基因功能参考文献：
    1. Studied maternal serum calponin 1 levels in the prediction of delivery within 7 days among pregnancies complicated with threatened preterm labor. Of 73 women included in the study, found the maternal serum calponin 1 level was significantly high in women who delivered within 7 days vs. those who delivered after 7 days. PMID: 28068849
    2. Loss of myoepithelial calponin was specifically associated with gain of the basal marker p63 in adjacent tumor cells. PMID: 26343330
    3. Loss of CNN1 expression is associated with Ovarian Cancer. PMID: 26504022
    4. CNN1 immunohistochemical staining allows ready recognition of intracytoplasmic inclusions, aiding diagnosis of infantile digital fibromatosis. PMID: 24117680
    5. epsilonPKC/ERK/mTOR pathway activation induced by the rescued expression of CNN1 contributed to the improvement of cardiac dysfunction and pathological changes observed in transgenic mice. PMID: 24631115
    6. Calponin knockdown inhibits autophosphorylation. PMID: 24350264
    7. Expression of transforming growth factor-beta (4.3 +/- 1.4 vs 2.6 +/- 2.3, p = 0.01) and calponin (3.7 +/- 0.7 vs 0.6 +/- 1.1, p = 0.05) was lower from infants with vs without a PDA. PMID: 23731614
    8. Calponin-1 expression in the uterine smooth muscle tissue of the body and the lower uterine segment of smooth muscle tissues was significantly different. PMID: 21051832
    9. Inhibition of calponin-1 expression can inhibit uterine smooth muscle cell migration and cause the morphological change and rearrangement of F-actin without affecting its proliferation and apoptosis in vitro. PMID: 20584679
    10. A single intronic CArG element is necessary but insufficient for proper CNN1 expression in vivo. CNN1 overexpression antagonizes arterial injury-induced neointimal formation. PMID: 21817093
    11. D2-40 immunohistochemistry reliably identifies the myoepithelial cells of breast in a variety of lesions in a pattern similar to that of calponin and p63. PMID: 21326813
    12. The point mutation S175T exhibited more resistance to actin rearrangement by cytochalasin D and PDBu than intact CNh1, suppressing cell motility induced by PDBu. PMID: 20530411
    13. expression in tumor vessels is a new and useful means to predict prognosis of hepatocellular carcinoma after hepatic resection PMID: 11920541
    14. results suggest that regulation of h1-calponin is involved in the regulation of differentiation of AR42J cells into insulin-producing cells PMID: 12606518
    15. the calponin homology domain of Vav1 associates with calmodulin and is prerequisite to T cell antigen receptor-induced calcium release in Jurkat T lymphocytes PMID: 17550897
    16. analysis of distinct sites of interaction that form the calponin: gelsolin complex and two calcium switches that control its activity PMID: 17556051
    17. expression of vascular CNN1 in the peripheral region of colon cancer tissues was significantly reduced in association with tumor progression, lymphatic invasion, vascular invasion and recurrence. PMID: 17707120
    18. Calponin and alpha-inhibin are the most useful positive markers for serous microcystic adenoma, and are negative in most entities in the differential diagnosis. PMID: 19391217

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  • 蛋白家族：
    Calponin family
  • 组织特异性：
    Smooth muscle, and tissues containing significant amounts of smooth muscle.
  • 数据库链接：

    HGNC: 2155

    OMIM: 600806

    KEGG: hsa:1264

    STRING: 9606.ENSP00000252456

    UniGene: Hs.465929