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  4. Human C4b-binding protein beta chain(C4BPB) ELISA kit

Human C4b-binding protein beta chain(C4BPB) ELISA kit

  • 中文名称：
    人C4b结合蛋白β链(C4BPB)酶联免疫试剂盒
  • 货号：
    CSB-EL003954HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human C4BPB ELISA Kit was designed for the quantitative measurement of Human C4BPB protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

  • 别名：
    C4b binding protein beta chain ELISA Kit; C4b-binding protein beta chain ELISA Kit; C4BP ELISA Kit; C4BPB ELISA Kit; C4BPB_HUMAN ELISA Kit; Complement component 4 binding protein beta ELISA Kit; Complement component 4 binding protein beta chain ELISA Kit; Complement component 4-binding protein beta ELISA Kit; OTTHUMP00000034288 ELISA Kit; OTTHUMP00000034289 ELISA Kit; OTTHUMP00000034375 ELISA Kit; OTTHUMP0000003474 ELISA Kit; Proline rich protein ELISA Kit; PRP ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C4BPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1000Average %96
    Range %91-108
    1:2000Average %92
    Range %87-99
    1:4000Average %94
    Range %89-98
    1:8000Average %97
    Range %91-112
  • 回收率：
    The recovery of human C4BPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-105
    EDTA plasma (n=4)9589-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.905 2.875 2.890 2.755
    1002.489 2.473 2.481 2.346
    501.734 1.750 1.742 1.607
    250.948 0.957 0.953 0.818
    12.50.512 0.506 0.509 0.374
    6.250.464 0.471 0.468 0.333
    3.1250.230 0.234 0.232 0.097
    00.133 0.136 0.135
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.
  • 基因功能参考文献：
    1. SAA, PROZ, and C4BPB may serve as new potential biomarkers for TB PMID: 28278182
    2. Binding of complement inhibitor C4b-binding protein to a highly virulent Streptococcus pyogenes M1 strain is mediated by protein H and enhances adhesion to and invasion of endothelial cells. PMID: 24064215
    3. crystallographic data on the structure of CD11c/CD18 and prediction of the secondary structure of the C4BP oligomerization domain, we show that epitopes bound by KIM185 in these proteins are unlikely to share any major structural similarity. PMID: 21856004
    4. C4BP alpha7beta0 subunit [C4BP alpha7beta0 isoform lacking the beta-chain] induces a semimature, tolerogenic state on human monocyte-derived dendritic cells activated by a proinflammatory stimulus. PMID: 23390292
    5. Since FI requires cofactors for its activity we also investigated the binding of purified cofactors C4BP and FH and found acquisition of both proteins, which retained their activity in FI mediated degradation of C3b and C4b. PMID: 22514678
    6. Results demonstrate that successful secretion of complement regulatory protein C4b-binding protein beta-chains depends on intracellular complex formation with vitamin K-dependent protein S, but not on the alpha-chains. PMID: 20693287
    7. Localized via protein S to the surface of apoptotic cells, C4BP is able to interact with complement protein C4b, thus retaining its physiological role in regulation of complement on the apoptotic cell surface. PMID: 12193728
    8. C4BP is composed of seven alpha-chains and a unique beta-chain, each chain comprising repeating complement control protein (CCP) modules, the results suggest that the role of CCP2 in protein S binding is to orient and stabilize CCP1 PMID: 12492479
    9. C4b-binding protein binds to necrotic cells and DNA, limiting DNA release and inhibiting complement activation. PMID: 15967823
    10. analysis of structural basis for C4b-binding protein interaction with streptococcal M protein PMID: 16330538
    11. review of multiple functional roles of the complex of protein S and cd4-binding protein beta chain, such as binding to apoptotoic cells, phagocytosis PMID: 17597997
    12. C4BP levels are increased in patients suffering from primary Sjogren's syndrome PMID: 19284503

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  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    HGNC: 1328

    OMIM: 120831

    KEGG: hsa:725

    STRING: 9606.ENSP00000243611

    UniGene: Hs.99886