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  4. Human C1q/TNF-related protein-3,Cartonectin/CTRP3/CORS-26 ELISA Kit

Human C1q/TNF-related protein-3,Cartonectin/CTRP3/CORS-26 ELISA Kit

  • 中文名称：
    人C1q/TNF相关蛋白3(Cartonectin/CTRP3/CORS-26)酶联免疫试剂盒
  • 货号：
    CSB-E13426h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human CTRP3 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human CTRP3 in multiple samples, including serum, plasma, or cell culture supernates. Antibody specific for CTRP3 has been pre-coated onto the microplate. Standards and samples are pipetted into the wells and any CTRP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated CTRP3 antibody is added to the wells. After washing, avidin conjugated HRP is added to the wells, forming an antibody-antigen-enzyme-labeled antibody complex. Following a wash to remove any unbound HRP-avidin, the TMB substrate solution is added to the wells, and the color develops into blue. The color changes from blue to yellow after the addition of stop solution into the wells. The color intensity is in proportion to the amount of CTRP3 bound in the initial step.

    CTRP3 (C1QTNF3) is one of the anti-inflammatory adipocytokines and inversely correlates with pro-inflammatory cytokines such as IL-6, TNF, and CRP. It is involved in various physiological and pathological processes, including glucose/lipid metabolism, inflammation, proliferation, apoptosis, vascular calcification, fibrosis, ischemic injury, and cardiovascular protection. CTRP3 also influences some specific inflammatory disorders, such as immunoglobulin A nephropathy and inflammatory bowel disease. Apart from its role in inflammation, CTRP3 affects the differentiation and adipogenesis of adipocytes.

  • 别名：
    C1ATNF3 ELISA Kit; C1q and tumor necrosis factor related protein 3 ELISA Kit; C1QT3_HUMAN ELISA Kit; C1qtnf3 ELISA Kit; Cartonectin ELISA Kit; Collagenous repeat containing sequence of 26 kDa ELISA Kit; collagenous repeat-containing sequence 26 kDa protein ELISA Kit; Complement C1q tumor necrosis factor related protein 3 ELISA Kit; Complement C1q tumor necrosis factor-related protein 3 ELISA Kit; Corcs ELISA Kit; CORS 26 ELISA Kit; Cors ELISA Kit; CORS26 ELISA Kit; CTRP3 ELISA Kit; FLJ37576 ELISA Kit; OTTHUMP00000115918 ELISA Kit; OTTHUMP00000219981 ELISA Kit; PRO1484 ELISA Kit; Secretory protein CORS26 ELISA Kit; UNQ753 ELISA Kit
  • 缩写：
    C1QTNF3
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CTRP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %81-95
    1:2Average %92
    Range %90-102
    1:4Average %96
    Range %91-100
    1:8Average %95
    Range %89-102
  • 回收率：
    The recovery of human CTRP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9385-100
    EDTA plasma (n=4)10190-110
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    101.839 1.952 1.896 1.741
    51.616 1.586 1.601 1.446
    2.51.354 1.271 1.313 1.158
    1.250.912 0.932 0.922 0.767
    0.6250.617 0.609 0.613 0.458
    0.3120.412 0.388 0.400 0.245
    0.1560.263 0.251 0.257 0.102
    00.159 0.151 0.155 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 基因功能参考文献：
    1. we have developed a diagnostic and prognostic prediction model for Prostate cancer. C1QTNF3 was revealed as a promising biomarker for Prostate cancer PMID: 29861410
    2. CTRP3 may be a promising therapeutic target for the treatment of liver fibrosis. PMID: 28320106
    3. Elevated serum CTRP 3 levels were closely related to the prevalence and severity of coronary artery disease, suggesting that it might be regarded as a novel biomarker for CAD. PMID: 28754090
    4. defensive roles in suppressing inflammation and fibrosis in polymeric IgA complex-stimulated mesangial cells; may target the NF-kappaB and TGF-beta/Src-Smad3 signaling pathways to play multipotent roles in relieving the pathological progression of IgA nephropathy PMID: 27309491
    5. CTRP3 promotes mitochondrial biogenesis in cardiomyocytes via AMPK/PGC-1alpha pathway. PMID: 27793739
    6. Low CTRP3 expression is associated with diabetic retinopathy. PMID: 28632765
    7. decreased levels of CTRP3 and especially CTRP13 were associated with increased risk of T2DM and CAD PMID: 28033351
    8. both plasma CTRP-3 and HMGB-1 levels were significantly associated with pre-diabetes mellitus and newly diagnosed type 2 diabetes after adjusting for several confounders PMID: 27738641
    9. This research suggested that CTRP3 might protect chondrocytes against IL-1beta-induced osteoarthritis in a cell model by suppressing the FGFR1- Ras/PI3K/Akt signaling-mediated growth inhibitory pathway. PMID: 27930985
    10. CTRP3 could improve insulin sensitivity of insulin resistant 3T3-L1 adipocytes by decreasing inflammation and ameliorating insulin signalling transduction, indicating that CTRP3 may be a new target for the prevention and cure of insulin resistance and type 2 diabetes. PMID: 25185846
    11. CTRP3 is present in cord blood and might be involved in fetal growth PMID: 26656444
    12. CTRP3 overexpression altered chemokine levels and attenuated systemic inflammation in the setting of obesity. PMID: 26997632
    13. Lower Circulating C1q/TNF-Related Protein-3 (CTRP3) Levels Are Associated with Obesity PMID: 26222183
    14. plasma CTRP-3 is strongly associated with glucose and lipid metabolism, chronic inflammation, and insulin resistance. PMID: 26073386
    15. Results show that cartonectin may serve as a novel biomarker for the prediction and early diagnosis of type 2 diabetes mellitus patients. PMID: 25409499
    16. Patients with acute coronary syndrome or stable angina pectoris had significantly lower circulating CTRP-3 concentrations compared to controls. PMID: 24417980
    17. CTRP3 promotes vascular calcification by enhancing phosphate-induced osteogenic transition of VSMC through reactive oxygen species-extracellular signal-regulated kinase 1/2-Runx2 pathway. PMID: 24578384
    18. Data suggest that serum/omental adipose tissue (AT) cartonectin levels are lower in women with polycystic ovary syndrome; treatment with a hypoglycemic agent (metformin) increases serum cartonectin levels in these women and in omental AT explants. PMID: 24152681
    19. Data suggest that CTRP3 is expressed in subcutaneous and visceral adipocytes; CTRP3 appears to play important roles in adipocyte (epinephrine-induced) lipolysis, inflammation/infection, and adipokine/resistin secretion. PMID: 23174996
    20. Data suggest that patients exhibit significantly elevated circulating CTRP-3 in type 2 diabetes or prediabetes compared with subjects with normal glucose tolerance. Plasma CTRP-3 might be useful biomarker for atherosclerosis. PMID: 22837306
    21. This study provides the first functional evidence linking CTRP3 to hepatic glucose metabolism and establishes CTRP3 as a novel adipokine. PMID: 20952387
    22. CTRP-3 inhibits three basic and common proinflammatory pathways involved in obesity and type 2 diabetes mellitus (adipo-inflammation) by acting as an endogenous LPS antagonist of the adipose tissue. PMID: 20739398
    23. Genotyping of the shared variants in a Puerto Rican sample of 118 cases and 136 controls did not reveal either allelic or genotype association with schizophrenia. PMID: 20483475
    24. Blood level is determined by a glucose tolerance test in normal adults PMID: 17311679
    25. CTRP3/cartducin may be involved as a novel angiogenic factor in the formation of neointima following angioplasty. PMID: 17534697
    26. CORS26/cartonectin is a new adipokine that differentially regulates the secretion of classical adipokines, with marked differences between the human and the murine systems. PMID: 18421280

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Expressed in colon and small intestine.
  • 数据库链接：

    HGNC: 14326

    OMIM: 612045

    KEGG: hsa:114899

    STRING: 9606.ENSP00000371497

    UniGene: Hs.171929