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  4. Human Bromodomain testis-specific protein(BRDT) ELISA kit

Human Bromodomain testis-specific protein(BRDT) ELISA kit

  • 中文名称：
    人溴结构域睾丸特异蛋白(BRDT)酶联免疫试剂盒
  • 货号：
    CSB-EL002807HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human BRDT ELISA Kit was designed for the quantitative measurement of Human BRDT protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    BRDT ELISA Kit; Bromodomain testis-specific protein ELISA Kit; Cancer/testis antigen 9 ELISA Kit; CT9 ELISA Kit; RING3-like protein ELISA Kit
  • 缩写：
    BRDT
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human BRDT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-96
    1:2 Average % 87
    Range % 82-91
    1:4 Average % 105
    Range % 101-113
    1:8 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of human BRDT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 84 80-88
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 2.751 2.593 2.672 2.541
    2000 1.875 1.758 1.817 1.686
    1000 1.040 1.070 1.055 0.924
    500 0.592 0.611 0.602 0.471
    250 0.364 0.349 0.357 0.226
    125 0.239 0.227 0.233 0.102
    62.5 0.158 0.165 0.162 0.031
    0 0.127 0.134 0.131 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
  • 基因功能参考文献：
    1. FBP1 modulates the sensitivity of pancreatic cancer cells to BET inhibitors by decreasing the expression of c-Myc. These findings highlight FBP1 could be used as a therapeutic niche for patient-tailored therapies PMID: 30201002
    2. The bromodomain and extraterminal domain (BET) family consists of BRDT, BRD2, BRD3, and BRD4, each containing 2 bromodomains located N-terminal to extraterminal domain, which is located near C-terminus. Data suggest that 10 distinct acylations participate in binding of BET family proteins to histone 4 (oligopeptide fragments used here); C-terminal bromodomains do not cooperatively bind multiple acylation sites. PMID: 28945351
    3. The affected patient carries a homozygous bromodomain, testis-specific protein (BRDT) mutation, while the patient's father, mother and elder brother all carry heterozygous BRDT mutations. PMID: 28199965
    4. considered potential associations of 14 single nucleotide polymorphisms (SNPs) in RNF8 and BRDT genes in Chinese patients with non-obstructive azoospermia PMID: 25374327
    5. assessment of rs3088232 frequency in large group of non-obstructive azoospermia men and fertile controls demonstrated no significant difference between them; conclude that testicular impairments observed were not a consequence of BRDT gene mutation PMID: 24865796
    6. In human, BRDT is the only BET gene expressed exclusively in testicular germ cells. PMID: 22035730
    7. Suggest that reduced histone methylation in the promoter of BRDT may be associated with increased transcript levels in subfertile patients. PMID: 20538714
    8. BRDT-NY gene is an alternatively spliced variant that may have an important role in the process of spermatogenesis and may be correlated with male infertility PMID: 15647849

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  • 相关疾病：
    Spermatogenic failure 21 (SPGF21)
  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Testis-specific. A 3-fold higher expression is seen in adult testis than in embryo testis. Expression seems to be correlated with histone H4 hyperacetylation during the haploid phase of spermatogenesis (spermiogenesis). No expression, or very low expressi
  • 数据库链接：

    HGNC: 1105

    OMIM: 602144

    KEGG: hsa:676

    STRING: 9606.ENSP00000354568

    UniGene: Hs.482520