Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Bone morphogenetic protein 3(BMP3) ELISA kit

Human Bone morphogenetic protein 3(BMP3) ELISA kit

  • 中文名称：
    人骨形态发生蛋白3(BMP3)酶联免疫试剂盒
  • 货号：
    CSB-EL002739HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human BMP3 ELISA Kit was designed for the quantitative measurement of Human BMP3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    BMP-3 ELISA Kit; BMP-3A ELISA Kit; bmp3 ELISA Kit; BMP3_HUMAN ELISA Kit; BMP3A ELISA Kit; Bone morphogenetic protein 3 (osteogenic) ELISA Kit; Bone morphogenetic protein 3 ELISA Kit; Bone morphogenetic protein 3A ELISA Kit; Osteogenin ELISA Kit
  • 缩写：
    BMP3
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human BMP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 88
    Range % 82-91
    1:2 Average % 102
    Range % 97-106
    1:4 Average % 94
    Range % 87-98
    1:8 Average % 93
    Range % 86-97
  • 回收率：
    The recovery of human BMP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 99-108
    EDTA plasma (n=4) 92 87-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.512 2.633 2.573 2.399
    500 1.834 1.948 1.891 1.717
    250 1.176 1.218 1.197 1.023
    125 0.756 0.774 0.765 0.591
    62.5 0.463 0.473 0.468 0.294
    31.2 0.289 0.296 0.293 0.119
    15.6 0.225 0.235 0.230 0.056
    0 0.173 0.175 0.174 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Growth factor of the TGF-beta superfamily that plays an essential role in developmental process by inducing and patterning early skeletal formation and by negatively regulating bone density. Antagonizes the ability of certain osteogenic BMPs to induce osteoprogenitor differentitation and ossification. Initiates signaling cascades by associating with type II receptor ACVR2B to activate SMAD2-dependent and SMAD-independent signaling cascades including TAK1 and JNK pathways.
  • 基因功能参考文献：
    1. This study demonstrated that there was a significantly higher frequency of BMP3 methylated DNA in plasma in patients with polyps versus healthy controls with a sensitivity and specificity of 40 and 94%, respectively. PMID: 29892846
    2. BMP3 - the biomarker of a currently in-use multi-target stool DNA test was commonly expressed in tumor tissue specimens, independent of Fecal Immunochemical Test result. PMID: 28044229
    3. Methylation level in stool decreases dramatically following colorectal cancer resection PMID: 24993691
    4. Bone morphogenic protein 3 signaling in the regulation of osteogenesis. PMID: 23127436
    5. As an addition to PRKG2 and RASGEFIB genes, we propose to include BMP3 gene as the principal determinant of the observed common phenotype. PMID: 22303795
    6. a critical link between HNF1A-MODY-induced alterations in Bmp-3 expression and insulin gene levels PMID: 21628466
    7. Features supportive of positive selection in the BMP3 gene were found including the presence of an excess of nonsynonymous mutations in modern humans, and a significantly lower genetic diversity that deviates from neutrality PMID: 20532035
    8. Data suggest that promotor region methylation of the BMP3 gene may cause gastric carcinoma in Chinese people. PMID: 20238409
    9. These results demonstrate that BMP-3 stimulates mesenchymal stem cell proliferation via the TGF-beta/activin signaling pathway. PMID: 20143330
    10. The expression signals of BMP3 mRNA in malignant schwannoma were relatively lower than in benign lesions PMID: 11642720
    11. BMP-3 receptor specificity is controlled by the interaction of Lys-30 of BMP-3 with Glu-76 of ActRIIb PMID: 17924656
    12. BMP3 silencing is an early and frequent event in colorectal tumors progressing via the serrated and traditional pathways. PMID: 18311777

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 组织特异性：
    Expressed in adult and fetal cartilage.
  • 数据库链接：

    HGNC: 1070

    OMIM: 112263

    KEGG: hsa:651

    STRING: 9606.ENSP00000282701

    UniGene: Hs.387411