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  4. Human Aspartate aminotransferase,AST ELISA Kit

Human Aspartate aminotransferase,AST ELISA Kit

  • 中文名称：
    人天门冬氨酸氨基转移酶(AST)酶联免疫试剂盒
  • 货号：
    CSB-E09603h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        天冬氨酸氨基转移酶1（Glutamic-Oxaloacetic Transaminase 1），属于转移酶家族，参与氨基酸代谢和糖异生过程。GOT1广泛分布于肝、心脏、肌肉和肾等组织，是评价肝脏和心脏等组织损伤的生物标志物。对于肝脏疾病的检测，GOT1的检测灵敏度比AST更高，但AST更具特异性。
        j9九游会登录入口首页生物所提供的Human Aspartate aminotransferase,AST ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的GOT1，其灵敏度为0.078 mU/ml，检测范围为0.312 mU/ml - 20 mU/ml。
     
  • 别名：
    AATC_HUMAN ELISA Kit; Aspartate aminotransferase 1 ELISA Kit; Aspartate aminotransferase ELISA Kit; Aspartate aminotransferase cytoplasmic ELISA Kit; Aspartate aminotransferase cytosolic ELISA Kit; ASTQTL1 ELISA Kit; cAspAT ELISA Kit; cCAT ELISA Kit; cysteine aminotransferase; cytoplasmic ELISA Kit; cysteine transaminase; cytoplasmic ELISA Kit; cytoplasmic ELISA Kit; ec 2.6.1.1 ELISA Kit; GIG 18 ELISA Kit; GIG18 ELISA Kit; Glutamate oxaloacetate transaminase 1 ELISA Kit; Glutamate oxaloacetate transaminase soluble ELISA Kit; Glutamic oxaloacetic transaminase 1 soluble ELISA Kit; GOT 1 ELISA Kit; GOT1 ELISA Kit; Growth inhibiting protein 18 ELISA Kit; SGOT ELISA Kit; Transaminase A ELISA Kit
  • 缩写：
    GOT1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 mU/ml - 20 mU/ml
  • 灵敏度：
    0.078 mU/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-88  
    1:2 Average % 93  
    Range % 88-94  
    1:4 Average % 95  
    Range % 90-99  
    1:8 Average % 103  
    Range % 97-110  
  • 回收率：
    The recovery of human AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-94  
                 
                 
  • 标准曲线：

      

  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I would like to know if the kit is free of human or animal byproducts, HIV/AIDS/HCV/HBV/infectious material.

A:
Thanks for your inquiry.The kit is developed for the human matrix to quantitatively detect the content of the corresponding protein.
Some components of the kit contain BSA,there is no HIV / AIDS / HCV / HBV / infective material.
All kits are for scientific research use, there is no infectious component and can be used in accordance with conventional biosecurity rules of operation.

靶点详情

  • 最新研究进展：
    GOT1，即天冬氨酸转氨酶1，是一种酶，参与氨基酸代谢。最新的研究表明，GOT1在某些癌症的发生和发展中发挥着重要作用。例如，研究表明，GOT1在肝癌和结肠癌中的表达水平升高，并且与肿瘤的分化程度和恶性程度密切相关。此外，一些研究表明，抑制GOT1的活性可能是一种治疗肝癌和结肠癌的新策略
  • 功能：
    Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
  • 基因功能参考文献：
    1. high transaminase elevations do not predict severe complications or reflect remnant ischemic area. PMID: 29553104
    2. Missense variant (p.Gln208Glu, rs374966349) in glutamate oxaloacetate transaminase 1 (GOT1) was found, as a putative causal variant predisposing to Familial Macro-aspartate aminotransferase . PMID: 28716744
    3. HIF-2a regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway and GOT1 expression in human pancreatic ductal adenocarcinoma. PMID: 28544376
    4. ALT is a more suitable index than AST for developing a regression model. PMID: 28628604
    5. High AST1 expression is associated with high mortality in hemodialysis patients. PMID: 26333544
    6. GOT1 plays an important role in energy metabolism and ROS balance in chronic acidosis stress in pancreatic tumor cells. PMID: 28455244
    7. Early abnormal liver enzyme levels of aspartate aminotransferase and alanine aminotransferase may increase the prevalence of human cytomegalovirus antigenemia after hematopoietic stem cell transplantation. PMID: 28415934
    8. Elevated preoperative aspartate aminotransferase-to-platelet ratio index may be independently associated with poor overall survival and disease-free survival in hepatocellular carcinoma patients following curative resection PMID: 28351330
    9. Elevated levels of ALT are Associated with Cardiovascular disease. PMID: 27872510
    10. Elevated aspartate aminotransferase level was associated with hepatocellular carcinoma. PMID: 26881519
    11. The serum hepcidin, ferritin, alanine transaminase, aspartate transaminase, gamma-glutamyltransferase and bilirubin were examined in two independent Chinese cohorts consisted of 3455 individuals. PMID: 26290281
    12. Data suggest that, in context of abnormal hepatic lipid accumulation in nonalcoholic fatty liver disease, circulating GOT1 rises due to up-regulation of hepatic expression of GOT1 associated with greater gluconeogenesis and insulin resistance. PMID: 26791191
    13. Human GOT1 was expressed in E coli, purified by affinity chromatography and preliminary crystallographic studies were done. PMID: 26003525
    14. We propose the GOT1 expression status as a simple and reliable prognostic biomarker in pancreatic ductal adenocarcinoma PMID: 25595905
    15. Significantly higher GOT levels were found in the 1-20 year age group of type 1 PMID: 24849491
    16. The aim of this study was to establish the reference intervals (RIs) of total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate transaminase (AST), and creatinine (CREA) for apparently healthy elderly (Han ethnicity) in Shuyang, China. PMID: 25272068
    17. APRI and AST/ALT are independent predictors for significant fibrosis in chronic hepatitis C patients. PMID: 22352690
    18. This study highlights the necessity of intensive follow-up for those with elevated AST and ALT levels and comorbid alcohol use disorder for preventing excessive natural deaths. PMID: 22242166
    19. we discovered a rare in-frame deletion in GOT1 gene, which inactivates cAST enzyme in the Old Order Amish. PMID: 21900944
    20. Data indicate that the percentages of CD4(+)CXCR5(+) TFH in IA patients were positively correlated with AST. PMID: 21750724
    21. liver enzymes, including AST, were significantly associated with IR according to direct clamp assessment, which were independent of the traditional risk factors in diabetic patients PMID: 20972737
    22. Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. PMID: 20499337
    23. mildly elevated in severe acute respiratory syndrome patients during second week after onset of fever. PMID: 15306699
    24. saliva AST and ALP may have roles in development of periodontal diseases PMID: 16681433
    25. PTLP activity is positively associated with serum ALT1, GOT1 and GOT2 in type 2 diabetes mellitus and metabolic syndrome. PMID: 17877759
    26. Abnormal serum aminotransferase values are uncommon in severely obese children in France. PMID: 17907318
    27. Data revealed higher aspartate aminotransferase for women with a family history of diabetes, when adjusted for age and BMI. PMID: 18242760
    28. Serum bilirubin, alkaline phosphatase, and aspartate aminotransferase are an efficient set of biochemistries to identify UDCA-treated patients with primary biliary cirrhosis at risk of death or liver transplantation (LT). PMID: 18752324
    29. Neither ALT nor GGT concentrations were correlated with ALP concentration, but AST concentration was moderately correlated with ALP concentration. PMID: 19012630
    30. Results suggest that alanine and aspartate aminotransferase levels and AST/ALT ratio do not seem to be reliable predictors for non-alcoholic steatohepatitis. PMID: 19370784
    31. the management of patients with pyelonephritis should take into account that moderate and self-limited abnormalities in aminotransferase levels are frequent during the acute phase of the disease PMID: 19393479

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family
  • 数据库链接：

    HGNC: 4432

    OMIM: 138180

    KEGG: hsa:2805

    STRING: 9606.ENSP00000359539

    UniGene: Hs.500756