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  4. Human Apolipoprotein M(APOM) ELISA kit

Human Apolipoprotein M(APOM) ELISA kit

  • 中文名称：
    人载脂蛋白M(APOM)酶联免疫试剂盒
  • 货号：
    CSB-EL001947HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    人载脂蛋白M(APOM)ELISA kit可用于血清、血浆、细胞培养上清液中人APOM蛋白的定量检测。该ELISA检测试剂盒采用双抗夹心法，其检测范围为1.56 ng/mL-100 ng/mL，灵敏度为0.39 ng/mL。

  • 别名：
    Apo M ELISA Kit; Apo-M ELISA Kit; Apolipoprotein M ELISA Kit; ApoM ELISA Kit; APOM_HUMAN ELISA Kit; G3A ELISA Kit; HSPC336 ELISA Kit; MGC22400 ELISA Kit; NG20 ELISA Kit; NG20 like protein ELISA Kit; Protein G3a ELISA Kit
  • 缩写：
    APOM
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 84  
    Range % 80-96  
    1:2000 Average % 95  
    Range % 92-102  
    1:4000 Average % 98  
    Range % 92-110  
    1:8000 Average % 95  
    Range % 86-100  
  • 回收率：
    The recovery of human APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-98  
    EDTA plasma (n=4) 96 92-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    100 2.566 2.547 2.557 2.464  
    50 2.011 1.995 2.003 1.910  
    25 1.409 1.394 1.402 1.309  
    12.5 0.867 0.896 0.882 0.789  
    6.25 0.524 0.502 0.513 0.420  
    3.12 0.345 0.364 0.355 0.262  
    1.56 0.213 0.217 0.215 0.122  
    0 0.094 0.091 0.093 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ApoM antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled ApoM antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
  • 基因功能参考文献：
    1. Obese patients showed significantly lower plasmatic ApoM levels than people with normal body weight, and ApoM level showed a strong correlation with CRP, TNF-alpha, and IL-6 levels, which indicated that ApoM might be regulated by these inflammatory factors. PMID: 30110274
    2. These results demonstrated that ApoM protein mass were clearly higher in the NSCLC tissues than in non-small cell lung cancer (NSCLC) tissues. Overexpression of ApoM could promote NSCLC cell proliferation and invasion in vitro and tumor growth in vivo, which might be via upregulating S1PR1 and activating the ERK1/2 and PI3K/AKT signaling pathways. PMID: 29750961
    3. results do not suggest a diagnostic role for ApoM plasma levels in patients with primary VTE. Moreover, the current study suggests that role of ApoM as a risk factor may differ for primary VTE and recurrent VTE in male patients. PMID: 28914078
    4. These S1P-induced enhancements in growth factors and chemotactic cytokines in retinal pigment epithelium cells were significantly inhibited by ApoM treatment. Additionally, in vivo experiments using a laser-induced choroidal neovascularization (CNV) murine model demonstrated that intravitreal ApoM injection significantly reduced the progression of CNV formation. PMID: 29301231
    5. The potential of mean force for sphingosine-1-phosphate unbinding from apoM reflected a large binding strength of more than 60 kJ/mol. This high unbinding free energy for sphingosine-1-phosphate underlines the observed specificity of the physiological effects of sphingosine-1-phosphate as it suggests that the spontaneous release of sphingosine-1-phosphate from apoM is unlikely. PMID: 27476912
    6. results demonstrated that lower APOM levels in SLE patients and correlated with disease activity. PMID: 28476116
    7. Sequenced the ApoM gene in recurrent venous thromboembolism (VTE) and identified six polymorphisms. ApoM rs805297 was significantly associated with higher risk of VTE recurrence in male but not in female patients. PMID: 27277397
    8. Single nucleotide polymorphism in ApoM gene is associated with chronic obstructive pulmonary disease. PMID: 28927745
    9. ApoM T-855C and T-778C polymorphisms were found to be associated with obesity by regulating HDL metabolism, and the T alleles of apoM T-778C were shown to be more strongly correlated. PMID: 28245483
    10. liver mRNA levels of apoM and apoA1 decreased strongly upon sepsis induction. PMID: 26990127
    11. 17beta-estradiol induced up-regulation of apoM in HepG2 cells is through an ER-alpha-dependent pathway involving ER-alpha binding element in the promoter of the apoM gene. PMID: 28359281
    12. A shift in ApoM/sphingosine 1-phosphate between HDL-particles in women with type 1 diabetes mellitus Is associated with impaired anti-inflammatory effects of the ApoM/S1P complex. PMID: 28385702
    13. ApoM-bound sphingosine-1-phosphate regulates adhesion molecule abundance, leukocyte-endothelial adhesion, and endothelial barrier function via sphingosine-1-phosphate receptor1. PMID: 27879252
    14. HDL-associated ApoM is anti-apoptotic by delivering sphingosine 1-phosphate to S1P1 and S1P3 receptors on vascular endothelium. PMID: 28179022
    15. Plasma apoM concentrations are higher in patients with hyperlipidaemia than in healthy controls. Low plasma apoM levels in patients with T2DM are likely caused by diabetes but are not induced by hyperlipidaemia. PMID: 27633510
    16. The polymorphism C-724del in the promoter region of the apoM gene could confer the risk of T2DM among eastern Han Chinese. Unfortunately, the lowing of plasma apoM levels of C-724del mutant allele carriers compared with the wide-type homozygotes carriers in T2DM patients was not statistically different in present study, so further researchs were needed by enlarging the sample PMID: 27576735
    17. ApoM rs805296 polymorphism may be a risk factor for developing coronary artery disease [meta-analysis] PMID: 26723879
    18. ApoM/HDL-C and apoM/apoA1 ratios could be used as indicators for identification of DN from healthy people and from T2DM patients. PMID: 28073663
    19. ApoM may be a biomarker of coronary artery disease. ApoM-855 T-->C substitution provides binding sites for AP-2alpha and reduces ApoM transcription activity PMID: 27841911
    20. Data indicate significant association between the single nucleotide polymorphism (SNP rs805296) of apolipoprotein M (ApoM) and the susceptibility to ankylosing spondylitis (AS) among Chinese Han population in Lanzhou. PMID: 27412944
    21. Serum apolipoprotein M was elevated in chronic obstructive pulmonary disease patients and increased gradually with chronic obstructive pulmonary disease severity. PMID: 27001252
    22. ApoM is excreted in the urine of children after cardiac surgery in children with acute kidney injury PMID: 26642098
    23. Hyperglycemia-induced downregulation of apolipoprotein M expression is not via the hexosamine pathway. PMID: 26377577
    24. ApoM is highly expressed in renal proximal tubule cells and is usually reabsorbed by giantin-associated proteins in a process, which is also affected in kidney disease. PMID: 25901639
    25. our findings present supportive evidence that ApoM is a regulator of human LRH-1 transcription, and further reveal the importance of ApoM as a critical regulator of bile acids metabolism PMID: 25987835
    26. -724 I/D polymorphism decreases the apoM promoter activity, down-regulates the apoM protein expression level, and increases the risk of myocardial infarction PMID: 25637426
    27. these findings demonstrated that apoM suppressed TNF-alpha-induced expression of ICAM-1 and VCAM-1 through inhibiting the activity of NF-kappaB. PMID: 26057873
    28. role of apoM in lipid metabolism and cardiometabolic diseases.(48-55) PMID: 25551802
    29. ApoM levels differ according to country of birth and are associated with IR and T2DM only in native-born Swedes, not Iraqis living in Sweden. PMID: 24984825
    30. Binary logistic regression analysis suggested that both apoM and apoAI mRNA may considered as independent risk factors for fetal macrosomia PMID: 25194312
    31. ApoM overexpression may have a potential role in improving insulin resistance in vivo and modulating apoM expression might be a future therapeutic strategy against insulin resistance in type 2 diabetes. PMID: 25144649
    32. Single nucleotide polymorphism in APOM is associated with lung cancer. PMID: 25187487
    33. the apolipoprotein M/HDL pathway may have a role in the pathogenesis of COPD and emphysema [meta-analysis] PMID: 23900982
    34. Decreased activities of apolipoprotein m promoter are associated with the susceptibility to coronary artery diseases. PMID: 24578614
    35. the functional single-nucleotide polymorphism APOM rs805297 G/T variant allele was associated with rheumatoid arthritis risk PMID: 23660425
    36. The apolipoprotein M rs805297 SNP is not associated with an increased risk for developing coronary artery disease, although it does independently correlate with dyslipidemia in Han Chinese individuals. PMID: 24341666
    37. Apolipoprotein M T-778C polymorphism is associated with serum lipid levels and the risk of coronary artery disease in the Chinese population. (Meta-analysis) PMID: 24040766
    38. Data indicate that palmitic acid induced suppression of apolipoprotein M (APOM) expression is mediated via the peroxisome proliferator-activated receptor beta/delta (PPARbeta/delta) pathway. PMID: 24508264
    39. The unique apoM/S1P-enriched plasma HDL may serve to deliver S1P to extrahepatic tissues for atheroprotection and may have other as yet unidentified functions. PMID: 24318881
    40. Apolipoprotein M as a chaperone for sphingosine-1-phosphate. [Review] PMID: 23652568
    41. Livers are involved in S1P dynamism, and it was suggested that apoM, produced from livers, increases circulating plasma S1P by augmenting the S1P output from livers and modifies extracellular S1P metabolism. PMID: 23664237
    42. Vascular endothelial growth factor downregulates apolipoprotein M expression PMID: 22877565
    43. Data support that the APOM C-1065A polymorphism is associated with increased risk for developing rheumatoid arthritis and dyslipidaemia in RA patients. PMID: 23190940
    44. The APOM gene rs707921 and rs707922 single nucleotide polymorphisms are associated with some serum lipid parameters. PMID: 23086303
    45. These data suggest that the plasma clearance of apoM, despite apoM primarily being associated with HDL, is influenced by LDL receptor-mediated clearance of apoB-containing particles. PMID: 22826357
    46. The present study demonstrated that apoM expression could be elevated by ABCA1 via the RXR/LXR pathway and LRH1 does not involve in the regulation of apoM by the activation of ABCA1, although the direct regulative pathway(s) between ABCA1 and apoM gene is still unknown yet. PMID: 22516753
    47. ApoM can bind oxidized phospholipids, increasing the antioxidant effect of HDL. PMID: 22204862
    48. Report plasma levels of sphingosine-1-phosphate and apolipoprotein M in patients with monogenic disorders of HDL metabolism. PMID: 21944699
    49. Single Nucleotide Polymorphism at the promoter region of the APOM gene is associated with rheumatoid arthritis. PMID: 21844665
    50. Raised ApoM levels in hepatitis B virus (HBV) infection may in turn suppress HBV replication PMID: 21875437

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  • 亚细胞定位：
    Secreted. Note=Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins (LDL).
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 组织特异性：
    Plasma protein. Expressed in liver and kidney.
  • 数据库链接：

    HGNC: 13916

    OMIM: 606907

    KEGG: hsa:55937

    STRING: 9606.ENSP00000365081

    UniGene: Hs.534468