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  4. Human Anterior gradient protein 3 homolog(AGR3) ELISA kit

Human Anterior gradient protein 3 homolog(AGR3) ELISA kit

  • 中文名称：
    人前梯度蛋白3同源物(AGR3)酶联免疫试剂盒
  • 货号：
    CSB-EL001459HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human AGR3 ELISA Kit was designed for the quantitative measurement of Human AGR3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

  • 别名：
    AG-3 ELISA Kit; AG3 ELISA Kit; Agr3 ELISA Kit; AGR3_HUMAN ELISA Kit; Anterior gradient 3; xenopus; homolog of ELISA Kit; Anterior gradient homolog 3 ELISA Kit; Anterior gradient homolog 3 (Xenopus laevis) ELISA Kit; anterior gradient protein 3 ELISA Kit; Anterior gradient protein 3 homolog ELISA Kit; BCMP11 ELISA Kit; Breast cancer membrane protein 11 ELISA Kit; Gm888 ELISA Kit; hAG 3 ELISA Kit; hAG-3 ELISA Kit; HAG3 ELISA Kit; OTTHUMP00000158512 ELISA Kit; PDIA18 ELISA Kit; Protein disulfide isomerase family A member 18 ELISA Kit
  • 缩写：
    AGR3
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AGR3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %80-89
    1:2Average %91
    Range %86-95
    1:4Average %94
    Range %90-98
    1:8Average %97
    Range %92-101
  • 回收率：
    The recovery of human AGR3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-95
    EDTA plasma (n=4)9489-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.713 2.662 2.688 2.515
    52.376 2.431 2.404 2.231
    2.51.934 2.001 1.968 1.795
    1.251.509 1.582 1.546 1.373
    0.6250.974 1.020 0.997 0.824
    0.3120.564 0.559 0.562 0.389
    0.1560.296 0.279 0.288 0.115
    00.178 0.167 0.173 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Required for calcium-mediated regulation of ciliary beat frequency and mucociliary clearance in the airway. Might be involved in the regulation of intracellular calcium in tracheal epithelial cells.
  • 基因功能参考文献：
    1. Results show that AGR3 was highly upregulated in low-grade serous carcinoma (LGSC) compared to high-grade serous carcinoma (HGSC). It can be use along with p53 for differential diagnosis of HGSC and LGSC. PMID: 29620196
    2. transcriptionally activated in hepatitis C virus-infected cells by HCV NS5A PMID: 25868611
    3. Data show the potential usability of anterior gradient homolog 3 (AGR3) and anterior gradient homolog 2 (AGR2) as biomarkers for blood-based early detection of breast cancer. PMID: 25875093
    4. AGR3 is a specialized member of the PDI family that plays an unexpected role in the regulation of CBF and mucociliary clearance in the airway. PMID: 25751668
    5. Relying on available data and in silico analyses, study shows that AGR2 and AGR3 proteins are co-expressed or uncoupled in context-dependent manners in diverse carcinomas and healthy tissues. PMID: 25666661
    6. AGR3 was is predominantly expressed in liver cholangiocytes but not present in liver hepatocytes, making this protain a good marker for differential diagnosis of intrahepatic cholangiocarcinoma PMID: 24747240
    7. AGR3 expression in ovarian cancer is independent of oestrogen-receptor expression. PMID: 22361111
    8. AGR3 could serve as a prognostic marker for survival in patients with low grade and high grade serous ovarian carcinomas. PMID: 21451362
    9. hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan [hAG-3 protein] PMID: 12592373
    10. specific induction of Hag2 and Hag3 during hormone-induced breast carcinomas further support developmental specificity for the PDI/ERp family members PMID: 15935701

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  • 亚细胞定位：
    Endoplasmic reticulum.
  • 蛋白家族：
    AGR family
  • 组织特异性：
    Expressed in the lung, in the ciliated cells of the airway epithelium. Expression increased with differentiation of airway epithelial cells. Not detected in the mucous cells. Expressed in ciliated cells in the oviduct. Also detected in stomach, colon, pro
  • 数据库链接：

    HGNC: 24167

    OMIM: 609482

    KEGG: hsa:155465

    STRING: 9606.ENSP00000308606

    UniGene: Hs.100686