Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1(SCYE1) ELISA kit

Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1(SCYE1) ELISA kit

  • 中文名称：
    人氨酰tRNA合成酶复合多功能相互作用蛋白1(SCYE1)酶联免疫试剂盒
  • 货号：
    CSB-EL020871HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human SCYE1 ELISA Kit was designed for the quantitative measurement of Human SCYE1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

  • 别名：
    AIMP 1 ELISA Kit; Aimp1 ELISA Kit; AIMP1/p43 ELISA Kit; AIMP1_HUMAN ELISA Kit; Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 ELISA Kit; ARS interacting multifunctional protein 1 ELISA Kit; EMAP 2 ELISA Kit; EMAP-2 ELISA Kit; EMAP-II ELISA Kit; EMAP2 ELISA Kit; EMAPII ELISA Kit; Endothelial monocyte activating polypeptide 2 ELISA Kit; Endothelial monocyte activating polypeptide ELISA Kit; Endothelial monocyte-activating polypeptide 2 ELISA Kit; Endothelial monocyte-activating polypeptide II ELISA Kit; HLD3 ELISA Kit; Multisynthase complex auxiliary component p43 ELISA Kit; Multisynthetase complex auxiliary component p43 ELISA Kit; OTTHUMP00000219673 ELISA Kit; p43 ELISA Kit; Scye 1 ELISA Kit; Scye1 ELISA Kit; Scye1, formerly ELISA Kit; Small inducible cytokine subfamily E member 1 ELISA Kit; Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) ELISA Kit; Small inducible cytokine subfamily E, member 1, formerly ELISA Kit
  • 缩写：
    SCYE1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SCYE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 102
    Range % 95-106
    1:2 Average % 101
    Range % 96-105
    1:4 Average % 89
    Range % 86-94
    1:8 Average % 94
    Range % 89-98
  • 回收率：
    The recovery of human SCYE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 98-108
    EDTA plasma (n=4) 93 86-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 1.976 1.933 1.955 1.800
    750 1.365 1.284 1.325 1.170
    375 0.872 0.828 0.850 0.695
    187.5 0.578 0.545 0.562 0.407
    94 0.388 0.361 0.375 0.220
    47 0.251 0.246 0.249 0.094
    23.5 0.219 0.215 0.217 0.062
    0 0.156 0.154 0.155 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
  • 基因功能参考文献：
    1. The increase of EMAP-II serum level reflects an endothelial dysfunction in type 1 diabetes, alteration of carbohydrate and lipid metabolism could influence of this pathway. PMID: 29537208
    2. This study showed the potential of the anti-AIMP1 antibody as a therapeutic option for AD treatment. Also, the finding of an association of blood AIMP1 levels with the MMSE score. PMID: 27906773
    3. systemic mRNA expression of AIMP1 strongly differentiated children who failed to achieve asthma control with triamcinolone administration PMID: 27665382
    4. p43 induces both the mRNA and protein expression of IP-10. PMID: 27574027
    5. Data indicate that AIMP1 downregulation using siRNA is a novel tool to restore TGF-beta signaling and thereby increases the chondrogenic potential of dedifferentiated/degenerated chondrocytes. PMID: 26890138
    6. two homozygous missense variants, p.(Gly299Arg) and p.(Val176Gly), in the gene AIMP1 that co-segregated with the phenotype, are reported. PMID: 26173967
    7. findings suggest EMAP II is elevated in type 1 diabetic patients, particularly those with micro-vascular complications; EMAP II levels are related to inflammation, glycemic control, albuminuria level of patients and the risk of micro-vascular complications PMID: 26142824
    8. AIMp1 promotes the proliferation and migration of fibroblasts/endothelial cells and importantly, pro-inflammatory gene expression in monocytes/macrophages and dendritic cells. PMID: 26325028
    9. Increased serum level of EMAP-II may be one of the pathways of endothelial dysfunction in type 1 diabetes. PMID: 27089716
    10. AIMP1 appears to function as a novel inhibitor of PPARgamma that regulates adipocyte differentiation by preventing the transcriptional activation of PPARgamma. PMID: 25146391
    11. Data indicate that HCV E2 glycoprotein interacts with cellular AIMP1/p43 protein and promotes cell surface expression of membrane glycoproteins gp96 and transforming growth factor beta TGF-beta signaling. PMID: 24816397
    12. Data indicate that the N terminus of Pro-EMAP II binds to its C terminus, arginyl-tRNA synthetase, and the neurofilament light subunit. PMID: 25724651
    13. EMAP2 is a multifunctional polypeptide with proinflammatory and antiangiogenic activity. (Review) PMID: 26040042
    14. Kisspeptin effect on endothelial monocyte activating polypeptide II (EMAP-II)-associated lymphocyte cell death and metastases in colorectal cancer patients PMID: 24395571
    15. interactions between the N-terminal domains of ArgRS and AIMP1 are important for the catalytic and noncatalytic activities of ArgRS and for the assembly of the higher-order MSC protein complex with ArgRS-GlnRS-AIMP1 PMID: 25288775
    16. vascular contribution to lung development and the implications that vascular mediators such as EMAP II have in distal lung formation during the vulnerable stage of alveolar genesis. PMID: 24619875
    17. AIMP1 peptide promotes the proliferation of BMMSCs by activating the beta-catenin/TCF complex via FGFR2-mediated activation of Akt, which leads to an increase in mesenchymal stem cells in peripheral blood. PMID: 23672191
    18. Downregulation of CD23 attenuated AIMP1-induced TNF-alpha secretion and AIMP1 binding. PMID: 22767513
    19. EMAP-II transcripts are upregulated in tumour cells in hypoxic conditions. PMID: 23082492
    20. EMAP II has specific intracellular effects, and that this intracellular function appears to antagonize its extracellular anti-angiogenic effects during fetal development and pulmonary disease progression. PMID: 22412987
    21. we propose that AIMP1 effect on EC adhesion is mediated by the assembly of a cytoskeletal protein complex on the cytosolic face of the cell membrane PMID: 21416500
    22. Decreased expression of AIMP1 in gastric and colorectal cancer tissues suggests that down-regulation of this protein may be related to inactivation of the tumor suppressor functions of AIMP proteins and might play a role in the development of GC and CRC. PMID: 21789020
    23. EMAPII is elevated in the BALF of active smokers and patients with COPD. Expression was increased in COPD lungs and emphysema. PMID: 21576822
    24. The known pathological functions together with abundant cellular accumulation in cerebral ischemia lesions suggest that EMAPII might contribute to pathophysiological consequences of cerebral ischemia. PMID: 21047177
    25. Through genome-wide homozygosity mapping and mutation analysis, we demonstrated in all affected individuals a homozygous frameshift mutation that fully abrogates the main active domain of AIMP1 PMID: 21092922
    26. autoantibodies of aminoacyl-tRNA synthetase can be used in the diagnosis of type I diabetes PMID: 20429837
    27. LPS induces the dissociation of gp96 from AIMP1 by phosphorylation through TLR4/MyD88-mediated JNK activation, resulting in translocation of gp96 on the cell surface. PMID: 20510162
    28. p43 is cleaved during apoptosis by calpains and released as a truncated protein that is harmless for the structure of the atherosclerotic plaque. PMID: 20413730
    29. AIMP1 may exert its antitumor activity by inducing tumor-suppressing cytokines. After a single intravenous injection, AMP1 exhibited a low clearance showing a one-compartmental disposition. PMID: 19573982
    30. These results suggest the potential interaction of p43/EMAP II with alpha-ATP synthase and its role in the proliferation of endothelial cells. PMID: 11741979
    31. temporal variation in EMAP II expression in the human endometrium across the menstrual cycle and localize expression to glandular epithelial, endothelial, and stromal cells. Moreover, EMAP II expression is negatively regulated by PGE(2). PMID: 12161535
    32. p43-induced TNF production was mediated by the activation of MAPK family members, ERK and p38 MAPK, and by IkappaB degradation leading to the activation of NFkappaB. PMID: 12543078
    33. Expression of endothelial monocyte activating polypeptide II is increased after spinal cord injury (SCI), suggesting a role for EMAP II in the pathophysiology of secondary injury following SCI. PMID: 14588117
    34. Membrane-bound and soluble EMAP-II appear to play multiple roles in the tumor microenvironment, one of which is to assist in immune evasion. PMID: 14688335
    35. EMAP-II causes a dose-dependent inhibition of proliferation and apoptosis in Jurkat T cells and mitogen-activated peripheral blood mononuclear cells, suggesting that EMAP-II constitutes a component of a novel, immunosuppressive pathway in solid tumors PMID: 14982944
    36. DOC1 might play a role in mediating some of the effects of EMAP-II on endothelial cells PMID: 15935955
    37. EMAP II is cleaved by MMP-9, elastase, and cathepsin L. PMID: 16674941
    38. Three-dimensional structure is projected from amino acid sequence. PMID: 17018011
    39. suggest that the cleavage of p43 and its cellular delocalization PMID: 17303557
    40. Arginyl t-RNA synthetase over-expression associates with a reduced AIMP1 secretion. Multicatalytic protease is involved in the generation of the mature cytokine, EMAP II. PMID: 17443684
    41. serum EMAP-II levels are significantly higher in patients with NSCLC than in healthy subjects and suggest it is of potential prognostic value PMID: 18621627
    42. Results suggest novel roles for EMAP II in modulating the migration of dendritic cells and suggest that these effects may modify Meth A tumor/host interactions. PMID: 18951814
    43. Inhibition of VEGF signaling is one possible antiangiogenic mechanism of EMAP II, which may explain its in vivo antitumor activity and delineate therapeutic strategies to enhance anti-VEGF therapy to inhibit tumor growth. PMID: 19002109
    44. one of the major anti-angiogenic functions of EMAP-II is exerted through its inhibition of the HIF-1alpha activities PMID: 19362550
    45. EMAP-II plays an important role in endothelial cell dysfunction related to graft-versus-host disease after allogeneic stem cell transplantation. PMID: 19565673
    46. this is the first report showing evidence for the chemoattractant properties of EMAPII on leech and human microglial cells PMID: 19917687
    47. Expression of the cleaved EMAP-II form in tumour-biopsies correlates with a good response of melanoma patients to TNF-based ILP. PMID: 16615114
    48. EMAP-II facilitates apoptotic signaling of TNF-R1 via mobilization of TRADD. PMID: 17051333

    显示更多

    收起更多

  • 相关疾病：
    Leukodystrophy, hypomyelinating, 3 (HLD3)
  • 亚细胞定位：
    Nucleus. Cytoplasm, cytosol. Secreted. Endoplasmic reticulum. Golgi apparatus.
  • 数据库链接：

    HGNC: 10648

    OMIM: 260600

    KEGG: hsa:9255

    STRING: 9606.ENSP00000378191

    UniGene: Hs.591680