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  4. Human Alpha-1-acid glycoprotein 1(ORM1) ELISA kit

Human Alpha-1-acid glycoprotein 1(ORM1) ELISA kit

  • 中文名称：
    人α-1-酸性糖蛋白1(ORM1)酶联免疫试剂盒
  • 货号：
    CSB-EL017237HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ORM1 ELISA Kit was designed for the quantitative measurement of Human ORM1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 ng/mL-2000 ng/mL and the sensitivity is 7.8 ng/mL.

  • 别名：
    A1AG1_HUMAN ELISA Kit; AGP 1 ELISA Kit; AGP A ELISA Kit; AGP ELISA Kit; AGP1 ELISA Kit; Alpha 1 acid glycoprotein ELISA Kit; Alpha-1-acid glycoprotein 1 ELISA Kit; alpha-1-AGP ELISA Kit; Epididymis secretory sperm binding protein Li 153w ELISA Kit; glycoprotein; alpha-1-acid; of serum ELISA Kit; HEL S 153w ELISA Kit; OMD 1 ELISA Kit; ORM ELISA Kit; ORM1 ELISA Kit; Orosomucoid 1 ELISA Kit; Orosomucoid-1 ELISA Kit
  • 缩写：
    ORM1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 ng/mL-2000 ng/mL
  • 灵敏度：
    7.8 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ORM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 100  
    Range % 92-104  
    1:200 Average % 87  
    Range % 82-91  
    1:400 Average % 94  
    Range % 91-97  
    1:800 Average % 96  
    Range % 92-100  
  • 回收率：
    The recovery of human ORM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 87-97  
    EDTA plasma (n=4) 96 90-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2000 0.140 0.134 0.137    
    1000 0.415 0.434 0.425    
    500 0.806 0.770 0.788    
    250 1.068 1.011 1.040    
    125 1.467 1.445 1.456    
    62.5 1.686 1.634 1.660    
    31.25 1.832 1.788 1.810    
    0 2.027 1.994 2.011    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
  • 基因功能参考文献：
    1. Studies suggest plasma alpha-1-acid glycoprotein (AAG) as a potential predictive biomarker of docetaxel non-haematological AEs namely oral mucositis and rash. PMID: 28554261
    2. Data provide evidence that Orm1 is induced in response to hepatic injury and executes liver regeneration by activating cell cycle progression in hepatocytes. PMID: 28927749
    3. Data suggest that serum haptoglobin, fetuin-A, platelet factor-4, hs-CRP (high sensitive-C-reactive protein), SAP (serum amyloid P), AGP (alpha1-acid glycoprotein) levels of adolescents with metabolic syndrome are significantly higher than those of controls subjects. PMID: 27754964
    4. Results indicate that advanced extranodal NK/T cell lymphoma (ENKL) patients with unfavourable treatment outcomes displayed higher levels of S100A9 and ORM1. PMID: 27021626
    5. Glycosylated Alpha-1-acid glycoprotein 1 is ostensibly expressed in lung cancer patients and is a potential lung cancer serum biomarker. PMID: 26563422
    6. Altogether, these results indicate that alpha1-3 fucosylated glycoforms of AGP are increased in PDAC and could be potentially regarded as a PDAC biomarker. PMID: 26563517
    7. The results of this study suggest that the expression of VEGF-A and ORM-1 may be associated with two mechanisms (angiogenesis and tumor structural viscosity) that may influence tumor growth in odontogenic myxoma. PMID: 26193215
    8. Data suggest that human serum albumin (HSA) might serve as a carrier in delivering chitooligomers to target tissues than alpha-1-glycoprotein (AGP) which has pharmacological importance PMID: 24359035
    9. ORM1 stimulates quiescent monocytes to polarize to M2b monocytes. PMID: 25689617
    10. Identify ORM genetic variations/haplotype structure associated with serum alpha-1-acid glycoprotein level and the pharmacokinetics of paclitaxel in Japanese cancer patients. PMID: 21638284
    11. Data indicate that the band intensity of sialic acid content in alpha-1 Acid glycoprotein (AGP) of alcoholic liver cirrhosis was found to be lower than that in pooled control group. PMID: 25408356
    12. These findings suggest that the ORM distal promoter region differentially regulates expression of ORM genes at basal level and in acute phase responses. PMID: 24389491
    13. Identified is the ORM1 gene, coding for orosomucoid, as a novel locus associated with lag time variability, reflecting the initiation process of thrombin generation. PMID: 24357727
    14. analysing the glycosylation of AGP in patients receiving methadone may aid in determining whether the therapy is likely to be effective. PMID: 23936770
    15. The ORM1 A113G polymorphism was associated with the PKs variability after taking telmsiartan, as well as ABCC2 C3972T. The heterozygotes of ORM1 113AG showed a larger AUC and a notable BP change(%) from the baseline compared with the wild-type. PMID: 23940561
    16. The ORM1 may be considered as a signaling molecule involved in the maintenance of tissue homeostasis and remodeling. PMID: 23973664
    17. A cross-sectional study was conducted to evaluate the relationship between periodontitis and the common systemic inflammatory markers in 32 morbidly obese patients. The severity of periodontitis was associated with the plasma level of orosomucoid. PMID: 23526947
    18. Drug-binding energetics of human alpha-1-acid glycoprotein. PMID: 23192962
    19. AGP has a direct effect in brain microvasculature and may play an important role in altering blood brain barrier integrity in inflammatory-related diseases. PMID: 22633841
    20. alpha(1)-Acid glycoprotein up-regulates CD163 via TLR4/CD14 protein pathway: possible protection against hemolysis-induced oxidative stress. PMID: 22807450
    21. change of the maintenance of three acute phase proteins: ceruloplasmin, alpha1-antitripsin and orosomucoid in an oral fluid and blood plasma at paradontitis and myocardial infarction PMID: 22708402
    22. Analyses of Nicaraguan population surveillance data suggest that preschool children with elevated AGP1 levels have higher prevalence of anemia than children with normal AGP1 levels. PMID: 22908695
    23. The binding properties of the polymyxin class of antibiotics for human alpha-1-acid glycoprotein (AGP) have been characterized. PMID: 22587817
    24. Urinary MCP1 and AGP are biomarkers of lupus nephritis in patients with juvenile-onset systemic lupus erythematosus, providing insight into its pathophysiology PMID: 22147846
    25. Characterization of 6-mercaptopurine binding site on human alpha1-acid glycoprotein (orosomucoid) using molecular docking. PMID: 22574522
    26. Leukocytospermia was associated with the alterations of terminal monosaccharide expression in human seminal fibronectin and alpha{1}-acid glycoprotein PMID: 22048274
    27. In this study, the potential of CZE-UV and CZE-ESI-MS analysis of intact AGP isoforms to study the correlation of this protein with bladder cancer is shown. PMID: 22216449
    28. Importance of pH and disulfide bridges on the structural and binding properties of human alpha-acid glycoprotein. PMID: 21621584
    29. Data suggest that GDC-0449 pharmacokinetics are mediated by AAG binding. PMID: 21300760
    30. The distribution of the AGP phenotypes did not differ significantly among the disease groups studied PMID: 21726491
    31. analysis of differences in drug-binding selectivity between two forms of human alpha1-acid glycoprotein genetic variants, the A and F1*S forms PMID: 21349832
    32. Characterized are more than 150 human Alpha-1-acid glycoprotein isoforms, differing both in the amino acid sequence and in the glycosylation. PMID: 20617306
    33. Almost all of the acute-phase proteins were closely related to rheumatoid arthritis activity (based on DAS28) and their places in the downgrade scale were as follows: CRP, Tf, AGP, Hp and AAT. PMID: 20371432
    34. ORM integrates inflammatory and metabolic signals to modulate immune responses to protect adipose tissue from excessive inflammation and thereby from metabolic dysfunction. PMID: 20442402
    35. ORM1*S/*S genotype predicted failure to complete a 6-week trial of antidepressants, whereas elevated plasma concentration of orosomucoid predicted failure to respond to antidepressant therapy at 6 weeks. PMID: 19395425
    36. The present study investigated enhanced fucosylation of AGP in the sera of chronic hepatitis B (HBV-CH) and hepatitis B cirrhosis (HBV-LC) patients. PMID: 19459043
    37. A site-directed mutagenesis study of drug-binding selectivity in genetic variants of AGP1 is reported. PMID: 19198000
    38. Different ORM1 phenotypes may affect the disposition of quinidine PMID: 11814462
    39. demonstrate that orosomucoid and/or its glycoforms affects thyroid cell function in vitro and that it does so by influencing the second messenger cAMP probably by interacting directly with the TSH receptor PMID: 11911961
    40. The effect of alpha2,6-linked sialic acid on anti-IgM antibody-induced apoptosis in Ramos cells. PMID: 11925509
    41. folds as a highly symmetrical all-beta protein dominated by a single eight-stranded antiparallel beta-sheet PMID: 12480518
    42. exerts significant effects on the pharmacokinetics, plasma concentrations, and intracellular distribution of imatinib in chronic myeloid leukemia patients PMID: 12576428
    43. tertiary structure analysis PMID: 15013397
    44. Urinary levels are increaed in normoalbuminuric type 2 diabetic patients. PMID: 15111541
    45. the N-linked glycosylation pattern of AGP was explored PMID: 16261636
    46. The binding of coumarin enantiomers to ORM1 is studied. PMID: 16290938
    47. The drug binding site of AGP was gained from circular dichroism and electronic absorption spectra. PMID: 17321687
    48. Alpha-1-acid glycoprotein 1 may be an endogenous ligand for Siglec-5 as a signaling molecule that participates directly in the regulation of neutrophil responses. PMID: 17675532
    49. Different ORM1 genotypes affect the protein binding percentage and the concentration of serum free nortriptyline. PMID: 17944232
    50. A different distribution of the area percentage of AGP forms is observed when comparing samples from diseased and healthy individuals, the most acidic AGP forms being present in a higher proportion in the samples from cancer patients. PMID: 17987628

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：

    HGNC: 8498

    OMIM: 138600

    KEGG: hsa:5004

    STRING: 9606.ENSP00000259396

    UniGene: Hs.522356