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  4. Human Afamin(AFM) ELISA kit

Human Afamin(AFM) ELISA kit

  • 中文名称：
    人Afamin(AFM)酶联免疫试剂盒
  • 货号：
    CSB-EL001419HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The product CSB-EL001419HU is a sandwich ELISA kit developed to measure levels of human Afamin (AFM) in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of AFM bound in the initial step.

    AFM is a human plasma vitamin E-binding glycoprotein mainly produced in the liver. It promotes vitamin E transport across the blood-brain barrier. AFM is also a tumor marker for ovarian cancer. AFM has been found strongly associated with all components of metabolic syndrome including visceral obesity and has been a potential early marker of metabolic syndrome. It also plays a role in anti-apoptotic cellular processes associated with oxidative stress and is linked to insulin resistance and other features of metabolic syndrome.

  • 别名：
    AFAM_HUMAN ELISA Kit; Afamin ELISA Kit; Afm ELISA Kit; ALB 2 ELISA Kit; ALB2 ELISA Kit; ALBA ELISA Kit; ALF ELISA Kit; Alpha albumin ELISA Kit; Alpha-Alb ELISA Kit; Alpha-albumin ELISA Kit
  • 缩写：
    AFM
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.9 ng/mL-250 ng/mL
  • 灵敏度：
    0.97 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AFM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %96
    Range %90-102
    1:2000Average %89
    Range %84-95
    1:4000Average %100
    Range %95-106
    1:8000Average %97
    Range %92-104
  • 回收率：
    The recovery of human AFM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8993-96
    EDTA plasma (n=4)10298-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2502.024 2.098 2.061 1.929
    1251.715 1.761 1.738 1.606
    62.51.334 1.364 1.349 1.217
    31.250.893 0.884 0.889 0.757
    15.60.567 0.546 0.557 0.425
    7.80.321 0.349 0.335 0.203
    3.90.261 0.246 0.254 0.122
    00.131 0.133 0.132 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Functions as carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.
  • 基因功能参考文献：
    1. The results showed significantly higher serum afamin levels in women with pre-eclampsia and gestational diabetes mellitus compared to healthy pregnant women. PMID: 29405972
    2. LDL apheresis moderately decreases the circulating levels of afamin parallel to lowering HDL-C and ApoA1 levels. PMID: 30247793
    3. The concentration of afamin decreases with the severity of alcoholic liver cirrhosis. PMID: 30260179
    4. Afamin binds palmitoylated serine 209 of Wnt3a, providing a structural basis how afamin solubilizes hydrophobic and poorly soluble Wnt proteins. PMID: 29153507
    5. Results provide evidence that afamin could promote glycometabolism by up-regulating the glucose metabolism key enzymes in papillary thyroid carcinoma. PMID: 27329154
    6. Data suggest that up-regulation of AFM levels in serum in women with polycystic ovary syndrome can be used as a biological markers of those women with increased risk of developing metabolic syndrome, especially those with insulin resistance. PMID: 25208973
    7. A linear increase in afamin during pregnancy may serve as basic reference for subsequent investigations of afamin in pregnancy-related disorders. PMID: 24768783
    8. Afamin levels were altered significantly in the peritoneal fluid of women with endometriosis compared with disease-free controls. PMID: 20858448
    9. Binding of bilirubin with human serum albumin was studied by absorption, fluorescence and CD spectroscopy PMID: 12063119
    10. The protein structure of afamin has been determined by homology modeling and its in vitro binding properties confirmed by docking calculations on the proposed structure. PMID: 12463752
    11. vitamin E-binding properties were confirmed; found abundant presence of this protein in plasma, extravascular fluids such as follicular, and cerebrospinal fluids PMID: 15952736
    12. afamin might be a new family member of binding/transport proteins contributing to alpha-tocopherol homeostasis at the blood-brain barrier PMID: 19046407
    13. Reduced Afamin expression is associated with ovarian cancer. PMID: 19336561

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 组织特异性：
    High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level).
  • 数据库链接：

    HGNC: 316

    OMIM: 104145

    KEGG: hsa:173

    STRING: 9606.ENSP00000226355

    UniGene: Hs.168718