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  4. Human Adenylyl cyclase-associated protein 1(CAP1) ELISA kit

Human Adenylyl cyclase-associated protein 1(CAP1) ELISA kit

  • 中文名称：
    人腺苷酸环化酶关联蛋白1(CAP1)酶联免疫试剂盒
  • 货号：
    CSB-EL004486HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CAP1 ELISA Kit was designed for the quantitative measurement of Human CAP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

  • 别名：
    Adenylyl cyclase associated protein 1 ELISA Kit; Adenylyl cyclase-associated protein 1 ELISA Kit; CAP 1 ELISA Kit; CAP ELISA Kit; CAP; adenylate cyclase associated protein 1 (yeast) ELISA Kit; CAP1 ELISA Kit; CAP1 PEN ELISA Kit; CAP1-PEN ELISA Kit; CAP1_HUMAN ELISA Kit
  • 缩写：
    CAP1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    25 pg/mL-1600 pg/mL
  • 灵敏度：
    6.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CAP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %87-97
    1:2Average %94
    Range %90-98
    1:4Average %104
    Range %100-107
    1:8Average %95
    Range %92-99
  • 回收率：
    The recovery of human CAP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8884-93
    EDTA plasma (n=4)9288-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    16002.104 2.268 2.186 2.083
    8001.578 1.590 1.584 1.481
    4001.163 1.152 1.158 1.055
    2000.707 0.727 0.717 0.614
    1000.495 0.474 0.485 0.382
    500.292 0.278 0.285 0.182
    250.196 0.189 0.193 0.090
    00.102 0.104 0.103
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
  • 基因功能参考文献：
    1. RETN and CAP1 polymorphisms and gene expression may be potential biomarkers for breast cancer risk PMID: 29641286
    2. Significant up-regulation of PBMCs CAP1, CD36 mRNA and plasma resistin found in significant coronary artery disease, as well as in nonsignificant coronary artery disease compared to control group, indicates that resistin could be able to exert its effects stronger on cells with up-regulated CAP1 mRNA thus contributing atherosclerosis development. PMID: 28707728
    3. REVIEW: summarizes information available to date about the structural organization, regulation of functional activity of adenylyl cyclase-associated protein 1 (CAP1), and its participation in cellular processes PMID: 29534668
    4. Results identify a role for CAP1 in regulating proliferative transformation of cancer cells, with ERK signaling playing pivotal roles in mediating both cell functions. PMID: 27173014
    5. CAP1 expression is markedly increased in human glioma and that downregulation of CAP1 in tumors may serve as a treatment for glioma patients. PMID: 27432289
    6. our results indicated that CAP1 participated in the molecular pathological process of glioma PMID: 26810579
    7. a high expression of CAP1 is involved in the pathogenesis of EOC, and that the downregulation of CAP1 in tumor cells may be a therapeutic target for the treatment of patients with EOC. PMID: 25652936
    8. This study demonstrated an inverse relationship between CAP1 and MMP-9 expression, high expression of MMP-9 and low expression of CAP1 in those with chronic obstructive pulmonary disease compared with the non-chronic obstructive pulmonary disease group. PMID: 25332229
    9. CAP1 is involved in the brain metastasis in non-small cell lung cancer.CAP1 overexpression is associated with poor prognosis in non-small cell lung cancer patients. PMID: 25371324
    10. Overexpression of CAP1 in monocytes enhanced the resistin-induced increased activity of the cAMP-dependent signaling. PMID: 24606903
    11. results indicate that upregulated expression of CAP1 might contribute heavily to the metastasis of hepatocellular carcinoma. PMID: 24359721
    12. Overexpression of CAP1 is associated with breast carcinoma. PMID: 24522810
    13. CAP1 might be a potential molecular targeted therapy for surgery and immune treatment. PMID: 24509166
    14. overexpression of CAP1 in lung cancer cells, particularly at the metastatic stage, may have significant clinical implications as a diagnostic/prognostic factor for lung cancer. PMID: 23842884
    15. CAP1 depletion also led to changes in cofilin phosphorylation and localization as well as activation of focal adhesion kinase (FAK) and enhanced cell spreading. PMID: 23737525
    16. Findings suggest that CAP1 (cyclase-associated protein 1) is involved in the metastasis of esophageal squamous cell carcinoma, and increased expression may indicate a poor prognosis. PMID: 23904342
    17. plays a key role in speeding up the turnover of actin filaments by effectively recycling cofilin and actin and through its effect on both ends of actin filament PMID: 11950878
    18. Based on an analysis of 1075 beta-thalassemia alleles the CAP+1 (A-->C) mutation constituted 3.2% of north Indians. PMID: 17900295
    19. The high turnover rate of CAP1 by MMP-9, comparable to that of gelatin as the natural extracellular substrate of this enzyme, may be critical to prevent pathological conditions associated with considerable cytolysis. PMID: 18671965
    20. These studies suggest that CAP1 provides a direct link from the actin cytoskeleton to the mitochondria by functioning as an actin shuttle. PMID: 18716285
    21. The overexpression of CAP1 in pancreatic cancers is demonstrated and the involvement of CAP1 in the aggressive behavior of pancreatic cancer cells is suggested. PMID: 19188911

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    CAP family
  • 数据库链接：

    HGNC: 20040

    OMIM: 617801

    KEGG: hsa:10487

    STRING: 9606.ENSP00000361878

    UniGene: Hs.370581