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  4. Human Acyloxyacyl hydrolase(AOAH) ELISA kit

Human Acyloxyacyl hydrolase(AOAH) ELISA kit

  • 中文名称：
    人酰基羧酸水解酶AOAH）酶联免疫试剂盒
  • 货号：
    CSB-EL001853HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human AOAH ELISA Kit was designed for the quantitative measurement of Human AOAH protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    Acyloxyacyl hydrolase (neutrophil) ELISA Kit; Acyloxyacyl hydrolase large subunit ELISA Kit; AOAH ELISA Kit; AOAH_HUMAN ELISA Kit
  • 缩写：
    AOAH
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AOAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 88
    Range % 81-96
    1:2 Average % 97
    Range % 91-103
    1:4 Average % 99
    Range % 82-106
    1:8 Average % 87
    Range % 81-92
  • 回收率：
    The recovery of human AOAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 93-102
    EDTA plasma (n=4) 101 94-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.680 2.769 2.725 2.620
    5 2.246 2.124 2.185 2.080
    2.5 1.495 1.387 1.441 1.336
    1.25 0.820 0.837 0.829 0.724
    0.625 0.475 0.486 0.481 0.376
    0.312 0.281 0.272 0.277 0.172
    0.156 0.204 0.210 0.207 0.102
    0 0.104 0.105 0.105 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria.
  • 基因功能参考文献：
    1. analysis of AOAH function through its crystal structure and complex with lipopolysaccharide PMID: 29343645
    2. AOAH - Gene involved in innate immunity that have been associated with Chronic Rhinosinusitis. PMID: 27888910
    3. AOAH rs60023210 variants may be associated with carotid intima-media thickness at bifurcation. PMID: 26319989
    4. Polymorphisms in RYBP and AOAH genes are associated with chronic rhinosinusitis in a Chinese population. PMID: 22723975
  • 亚细胞定位：
    Secreted. Cytoplasmic vesicle.
  • 数据库链接：

    HGNC: 548

    OMIM: 102593

    KEGG: hsa:313

    STRING: 9606.ENSP00000258749

    UniGene: Hs.488007