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  4. Human Acid sphingomyelinase-like phosphodiesterase 3b(SMPDL3B) ELISA kit

Human Acid sphingomyelinase-like phosphodiesterase 3b(SMPDL3B) ELISA kit

  • 中文名称：
    人酸性鞘磷脂样磷酸二酯酶3b(SMPDL3B)酶联免疫试剂盒
  • 货号：
    CSB-EL021850HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human SMPDL3B ELISA Kit was designed for the quantitative measurement of Human SMPDL3B protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    SMPDL3B ELISA kit; ASML3B ELISA kit; ASMLPDAcid sphingomyelinase-like phosphodiesterase 3b ELISA kit; ASM-like phosphodiesterase 3b ELISA kit; EC 3.1.4.- ELISA kit
  • 缩写：
    SMPDL3B
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SMPDL3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 86
    Range % 83-89
    1:2 Average % 92
    Range % 89-97
    1:4 Average % 100
    Range % 95-105
    1:8 Average % 90
    Range % 87-93
  • 回收率：
    The recovery of human SMPDL3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 100-108
    EDTA plasma (n=4) 99 95-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.243 2.102 2.173 2.031
    20 1.545 1.632 1.589 1.447
    10 1.149 1.201 1.175 1.033
    5 0.825 0.835 0.830 0.688
    2.5 0.505 0.531 0.518 0.376
    1.25 0.345 0.362 0.354 0.212
    0.625 0.224 0.215 0.220 0.078
    0 0.144 0.140 0.142 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling. Has in vitro phosphodiesterase activity, but the physiological substrate is unknown. Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro).
  • 基因功能参考文献：
    1. Glomerular immunoreactivity and urinary excretion of SMPDL3b in proteinuric nephrotic syndrome patients decreased compared with controls. PMID: 29081073
    2. These results demonstrate that radiation injury induces early cytoskeletal remodeling, down-regulation of SMPDL3b, and elevation of cellular ceramide levels. Overexpression of SMPDL3b and pretreatment with rituximab confer a radioprotective effect in cultured podocytes. PMID: 27836988
  • 亚细胞定位：
    Secreted. Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Acid sphingomyelinase family
  • 数据库链接：

    HGNC: 21416

    OMIM: 617737

    KEGG: hsa:27293

    STRING: 9606.ENSP00000363001

    UniGene: Hs.123659