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  4. Human ADP-ribosylation factor-like protein 8B(ARL8B) ELISA kit

Human ADP-ribosylation factor-like protein 8B(ARL8B) ELISA kit

  • 中文名称：
    人ADP核糖基化因子样蛋白8B(ARL8B)酶联免疫试剂盒
  • 货号：
    CSB-EL002100HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ARL8B ELISA Kit was designed for the quantitative measurement of Human ARL8B protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

  • 别名：
    ADP ribosylation factor like 10C ELISA Kit; ADP ribosylation factor like 8B ELISA Kit; ADP-ribosylation factor-like protein 10C ELISA Kit; ADP-ribosylation factor-like protein 8B ELISA Kit; ARL10C ELISA Kit; ARL8B ELISA Kit; ARL8B_HUMAN ELISA Kit; FLJ10702 ELISA Kit; Gie1 ELISA Kit; Novel small G protein indispensable for equal chromosome segregation 1 ELISA Kit
  • 缩写：
    ARL8B
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ARL8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5 Average % 96  
    Range % 90-100  
    1:10 Average % 93  
    Range % 89-97  
    1:20 Average % 104  
    Range % 99-109  
    1:40 Average % 84  
    Range % 80-88  
  • 回收率：
    The recovery of human ARL8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 95-104  
    EDTA plasma (n=4) 90 85-94  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.680 2.752 2.716 2.538  
    500 1.979 2.076 2.028 1.850  
    250 1.230 1.214 1.222 1.044  
    125 0.773 0.739 0.756 0.578  
    62.5 0.455 0.476 0.466 0.288  
    31.2 0.384 0.369 0.377 0.199  
    15.6 0.270 0.286 0.278 0.100  
    0 0.175 0.181 0.178    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of lysosomal positioning which is important for nutrient sensing, natural killer cell-mediated cytotoxicity and antigen presentation. Along with its effectors, orchestrates lysosomal transport and fusion. Localizes specifically to lysosomal membranes and mediates anterograde lysosomal motility by recruiting PLEKHM2, which in turn recruits the motor protein kinesin-1 on lysosomes. Required for lysosomal and cytolytic granule exocytosis. Critical factor involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells. In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function. Also acts as a regulator of endosome to lysosome trafficking pathways of special significance for host defense. Regulates cargo trafficking to lysosomes by binding to PLEKHM1 and recruiting the HOPS subunit VPS41, resulting in functional assembly of the HOPS complex on lysosomal membranes. Plays an important role in cargo delivery to lysosomes for antigen presentation and microbial killing. Directs the intersection of CD1d with lipid antigens in lysosomes, and plays a role in intersecting phagosomes with lysosomes to generate phagolysosomes that kill microbes. Involved in the process of MHC II presentation. Regulates the delivery of antigens to lysosomes and the formation of MHC II-peptide complexes through the recruitment of the HOPS complex to lysosomes allowing the fusion of late endosomes to lysosomes. May play a role in chromosome segregation.; (Microbial infection) During Mycobacterium tuberculosis (Mtb) infection, is required for plasma membrane repair by controlling the exocytosis of lysosomes in macrophages. ARL8B secretion pathway is crucial to control the type of cell death of the M. tuberculosis-infected macrophages, distinguishing avirulent from virulent Mtb induced necrotic cell death.; (Microbial infection) During infection, coronaviruses such as SARS-CoV-2 and the chaperone HSPA5/GRP78 are probably co-released through ARL8B-dependent lysosomal exocytic pathway for unconventional egress.
  • 基因功能参考文献：
    1. s present evidence that Arl8b facilitates lipid hydrolysis to maintain efficient metabolism for a proliferative capacity in low nutrient environments, suggesting a likely explanation for the complete inability of Arl8b-depleted tumor cells to grow in vivo. PMID: 27105540
    2. Data indicate that E3 ubiquitin-protein ligase RNF167 ubiquitinates Arl8B at the lysine residue K141 and reduces the level of the ADP-ribosylation factor-like 8B Arl8B protein. PMID: 27808481
    3. Arl8b regulates the association of the human HOPS complex with lysosomal membranes. PMID: 25908847
    4. a small GTP-binding protein, ADP-ribosylation factor-like 8b (Arl8b), is identified as a critical factor required for NK cell-mediated cytotoxicity. PMID: 24088571
    5. The s find that Arl8B is required for kinesin-1 recruitment to Salmonella-containing vacuoles, migration of the vacuoles to the cell periphery 24 h after infection and for cell-to-cell transfer of bacteria to neighbouring cells. PMID: 21824248
    6. a critical regulator of cargo delivery to lysosomes PMID: 21802320
    7. Results suggest that the novel GTPases Gie1 and Gie2 associate with microtubules, and might be involved in chromosome segregation. PMID: 15331635
    8. Arl8b is involved in trafficking processes for lysosomes. PMID: 16650381
    9. Data show that with MAK3 knockdown, p53 is stabilized and phosphorylated and there is a significant transcriptional activation of proapoptotic genes downstream of p53, and that localization of Arl8b is altered, suggesting that Arl8b is a Mak3 substrate. PMID: 19398576

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  • 亚细胞定位：
    Late endosome membrane. Lysosome membrane. Cytoplasm, cytoskeleton, spindle. Cell projection, axon. Cell junction, synapse. Cytolytic granule membrane.
  • 蛋白家族：
    Small GTPase superfamily, Arf family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 25564

    OMIM: 616596

    KEGG: hsa:55207

    STRING: 9606.ENSP00000256496

    UniGene: Hs.250009