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Guinea pig interleukin 13,IL-13 ELISA Kit

  • 中文名称:
    豚鼠白介素13(IL-13)酶联免疫试剂盒
  • 货号:
    CSB-E12916Gp
  • 规格:
    96T/48T
  • 价格:
    ¥3800/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Guinea pig IL13 ELISA Kit was designed for the quantitative measurement of Guinea pig IL13 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.5 pg/mL-300 pg/mL and the sensitivity is 3.75 pg/mL.

  • 缩写:
    IL-13
  • Uniprot No.:
  • 种属:
    Guinea Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    7.5 pg/mL-300 pg/mL
  • 灵敏度:
    3.75 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of Guinea pig IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 88
    Range % 83-92
    1:2 Average % 95
    Range % 85-105
    1:4 Average % 93
    Range % 86-102
    1:8 Average % 97
    Range % 92-110
  • 回收率:
    The recovery of Guinea pig IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 91-103
    EDTA plasma (n=4) 93 86-99
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average
    300 2.244 2.267 2.256
    150 1.173 1.161 1.167
    75 0.628 0.650 0.639
    37.5 0.359 0.343 0.351
    15 0.152 0.151 0.152
    7.5 0.083 0.079 0.081
  • 数据处理:
  • 货期:
    3-5 working days

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