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  4. Grouper Vitellogenin,VTG ELISA Kit

Grouper Vitellogenin,VTG ELISA Kit

  • 中文名称：
    石斑鱼卵黄蛋白原(VTG)酶联免疫试剂盒
  • 货号：
    CSB-E14116Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Grouper VTG ELISA Kit was designed for the quantitative measurement of Grouper VTG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 50 ng/mL-2000 ng/mL and the sensitivity is 50 ng/mL.

  • 别名：
    vtg1 ELISA Kit; Vitellogenin ELISA Kit; VTG) [Cleaved into: Lipovitellin I ELISA Kit; LVI); Phosvitin ELISA Kit; PV); Lipovitellin II ELISA Kit; LVII)] ELISA Kit
  • 缩写：
    VTG
  • Uniprot No.：
  • 种属：
    Grouper
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    50 ng/mL-2000 ng/mL
  • 灵敏度：
    50 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of grouper VTG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 84-97  
    1:2 Average % 97  
    Range % 91-102  
    1:4 Average % 95  
    Range % 88-103  
    1:8 Average % 96  
    Range % 91-101  
  • 回收率：
    The recovery of grouper VTG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-100  
    EDTA plasma (n=4) 95 91-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2000 0.174 0.175 0.175    
    1000 0.359 0.378 0.369    
    460 0.567 0.552 0.560    
    150 0.872 0.891 0.882    
    50 1.513 1.476 1.495    
    0 2.389 2.411 2.400    
  • 数据处理：
  • 货期：
    3-5 working days

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