Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Goat Tri-iodothyronine(T3) ELISA KIT

Goat Tri-iodothyronine(T3) ELISA KIT

  • 中文名称：
    山羊三碘甲状腺原氨酸(T3)酶联免疫试剂盒
  • 货号：
    CSB-E13385G
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        T3是一种甲状腺激素，对于机体的代谢和发育有重要影响。它通过结合甲状腺激素受体，影响基因转录和蛋白质合成。T3水平的调节对于机体内平衡的维持至关重要，过低或过高的T3水平都会对身体产生负面影响。因此，T3在甲状腺功能异常、代谢异常等疾病的诊断和治疗中具有重要作用。
        j9九游会登录入口首页生物所提供的Goat Tri-iodothyronine(T3) ELISA KIT属于ELISA检测试剂盒，采用竞争法定量检测山羊血清、血浆、组织匀浆样本中的T3，其灵敏度为0.5 ng/mL，检测范围为0.5 ng/mL-8 ng/mL。
     
  • 缩写：
    T3
  • 种属：
    Capra hircus (Goat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.5 ng/mL-8 ng/mL
  • 灵敏度：
    0.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat T3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 95    
    Range % 90-103    
    1:2 Average % 95    
    Range % 85-102    
    1:4 Average % 93    
    Range % 90-97    
    1:8 Average % 94    
    Range % 88-99    
  • 回收率：
    The recovery of goat T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 86-102    
    EDTA plasma (n=4) 96 92-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average 　    
    8 0.166 0.168 0.167 　    
    4 0.281 0.288 0.285 　    
    2 0.558 0.553 0.556 　    
    1 1.262 1.247 1.255 　    
    0.5 2.105 2.138 2.122 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I'm studying metabolic stress in dairy goats in the peripartum.
In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

A:
Thanks for your inquiry. We have the mentioned kit available.For example:
#CSB-E13385G,Goat Tri-iodothyronine(T3) ELISA KIT *96T (If duplicate done,22 kits is advised;If not,only 11 kits is ok)
the standard wells are S1-S5 and duplicate is advised.With a blank well,the number of standard wells is 5×2+1=11.The rest is for samples wells,85=96-11(If duplicate is done for sample,then the number will be the half of that.)
If you want to detect diary goat samples,we suggest you do pre-testing first.

靶点详情

  • 最新研究进展：
    近期研究表明，T3在许多重要的生理过程中扮演着关键角色，如代谢调节、心脏功能、神经发育和免疫系统等。此外，一些研究还发现，T3可能在肿瘤生长和转移中发挥一定作用，值得深入探究。